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  4. Recombinant Rat Solute carrier family 12 member 3 (Slc12a3), partial

Recombinant Rat Solute carrier family 12 member 3 (Slc12a3), partial

  • 中文名称：
    大鼠Slc12a3重组蛋白
  • 货号：
    CSB-YP021384RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大鼠Slc12a3重组蛋白
  • 货号：
    CSB-EP021384RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大鼠Slc12a3重组蛋白
  • 货号：
    CSB-EP021384RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大鼠Slc12a3重组蛋白
  • 货号：
    CSB-BP021384RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大鼠Slc12a3重组蛋白
  • 货号：
    CSB-MP021384RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Slc12a3
  • Uniprot No.：
    P55018
  • 别名：
    Slc12a3; Tsc; Solute carrier family 12 member 3; Na-Cl symporter; Thiazide-sensitive sodium-chloride cotransporter
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Electroneutral sodium and chloride ion cotransporter. In kidney distal convoluted tubules, key mediator of sodium and chloride reabsorption. Receptor for the proinflammatory cytokine IL18. Contributes to IL18-induced cytokine production, including IFNG, IL6, IL18 and CCL2, either independently of IL18R1, or as a complex with IL18R1.
  • 基因功能参考文献：
    1. Kidneys respond acutely to hyperkalemic challenges by decreasing the activity of NCC while increasing that of ENaC. PMID: 28356292
    2. Direct epithelial action of calcineurin inhibitor cyclosporine A is sufficient for the activation of renal NCC. PMID: 28003191
    3. Data demonstrate that increased levels of NE prevent dietary sodium-evoked suppression of the NCC, via an ANG II-independent mechanism, to stimulate the development of salt-sensitive hypertension. PMID: 26608659
    4. Despite high circulating aldosterone levels in pregnancy, the aldosterone-responsive transporter NCC is not increased in total or activated (phosphorylated) abundance or in apical localization in midpregnancy. PMID: 25925254
    5. Estradiol, progesterone, and prolactin increase renal NaCl cotransporter phosphorylation and activity. PMID: 25587121
    6. a model where NCC is constitutively cycled to the plasma membrane, and upon stimulation, it can be phosphorylated to both increase NCC activity and decrease NCC endocytosis, together increasing NaCl transport in the DCT. PMID: 24668812
    7. findings indicate that hyperinsulinaemia is an important driving force of NCC (Na(+)-Cl(-) co-transporter) activity in the metabolic syndrome with possible consequences for blood pressure regulation. PMID: 22651238
    8. observation supports that even a small, but chronic reduction of NCC or NKCC2 function results in a lower blood pressure and decreased risk of hypertension in otherwise healthy individuals in the general population PMID: 21157372
    9. We have confirmed the expression of NKCC1 in the rat lens and report for the first time the expression of NCC in lens fiber cells. PMID: 20458365
    10. Results suggest that the vasopressin-V(2) receptor-NCC signaling cascade is a novel effector system to adjust transepithelial NaCl reabsorption in the distal convoluted tubule. PMID: 20007345
    11. N-glycosylated at two sites, glycosylation essential for efficient function and surface expression, and elimination of glycosylation allows greater access of thiazide diuretics to binding site PMID: 12538726
    12. NO synthase inhibition did not block the decrease in NCC abundance normally seen with aldosterone escape. PMID: 12837683
    13. ion translocation and diuretic binding specificity are determined by the central hydrophobic domain for the Na(+)-Cl(-) cotransporter. PMID: 15149970
    14. NCC is expressed in olfactory epithelium and participates in generation of the excitatory receptor current. PMID: 15356206
    15. Both newly detected TSC C1784T and ADRB3 T727C are gene polymorphisms susceptible to the antihypertensive effect of thiazides in patients with essential hypertension PMID: 15824464
    16. Enhanced abundance of NCC was observed in the initial stage after bile duct ligation, followed by a marked decrease. ENaC transcription, translation or cell surface abundance was not increased after bile duct ligation. PMID: 15956070
    17. A high-salt (HS) diet provokes a rapid and persistent redistribution of NCC from apical to subapical membranes, a mechanism that would facilitate a homeostatic decrease in NaCl reabsorption in the DCT to compensate for increased dietary salt. PMID: 16554416
    18. binding sites for chloride and thiazide in NCC are different PMID: 16624820
    19. NCC is activated by a mechanism that involves amino-terminal domain phosphorylation PMID: 16887815
    20. ANG II provokes rapid trafficking of Na+Cl- cotransporter from stores in subapical vesicles to the plasma membrane PMID: 17507603
    21. This is the first report confirming the expression of thiazide-sensitive Na(+), Cl(-) cotransporter in the endolymphatic sac. PMID: 18440307
    22. In salt-sensitive hypertension, there is a strong upregulation, both of NCC and ClC-K along the distal convoluted tubules, which explains the persistence of hypertension. PMID: 18480177
    23. the amino terminus of the WNKs contains the sequences that are required for their activating or inhibiting properties on NCC PMID: 18701621
    24. Distal tubule NCC may participate in pressure natriuresis-diuresis by retraction out of apical plasma membranes and that the retraction is, at least in part, driven by the fall in ANG II that accompanies acute hypertension. PMID: 19144688
    25. Altered renal expression of NCC in protein-deprived rats. PMID: 19202345

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein. Apical cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    SLC12A transporter family
  • 组织特异性：
    Expressed in kidney, predominantly in the outer cortex in short tubule segments.
  • 数据库链接：

    KEGG: rno:54300

    STRING: 10116.ENSRNOP00000025377

    UniGene: Rn.10467

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