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  4. Recombinant Rat [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2, mitochondrial (Pdk2), partial

Recombinant Rat [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2, mitochondrial (Pdk2), partial

  • 中文名称：
    大鼠Pdk2重组蛋白
  • 货号：
    CSB-YP727335RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大鼠Pdk2重组蛋白
  • 货号：
    CSB-EP727335RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大鼠Pdk2重组蛋白
  • 货号：
    CSB-EP727335RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大鼠Pdk2重组蛋白
  • 货号：
    CSB-BP727335RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大鼠Pdk2重组蛋白
  • 货号：
    CSB-MP727335RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Pdk2
  • Uniprot No.：
    Q64536
  • 别名：
    Pdk2; [Pyruvate dehydrogenase; acetyl-transferring)] kinase isozyme 2; mitochondrial; EC 2.7.11.2; PDK P45; Pyruvate dehydrogenase kinase isoform 2; PDH kinase 2
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Partial
  • 表达区域：
    -
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Kinase that plays a key role in the regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism. Mediates cellular responses to insulin. Plays an important role in maintaining normal blood glucose levels and in metabolic adaptation to nutrient availability. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. Plays a role in the regulation of cell proliferation and in resistance to apoptosis under oxidative stress. Plays a role in p53/TP53-mediated apoptosis.
  • 基因功能参考文献：
    1. PDK2/4 induction and the subsequent lactate surge induce the metabolic shift in the diabetic dorsal root ganglion thereby contributing to the pathogenesis of painful diabetic neuropathy. PMID: 26769971
    2. The Pdk2 and Pdk1 interaction site was identified with nuclear magnetic resonance and mass spectrometry. PMID: 25436986
    3. Inactivation of pyruvate dehydrogenase kinase 2 by mitochondrial reactive oxygen species. PMID: 22910903
    4. The results show that hepatic PDK4 up-regulation can be achieved by heterodimerization of either PPARalpha or TR with the RXR receptor and that effects of PPARalpha activation on hepatic PDK2 and PDK4 expression favour a switch towards expression of PDK4. PMID: 12435272
    5. mRNA levels not changed in skeletal muscle during fasting PMID: 14966024
    6. Carboxy-terminal truncation variants of PDK2 display a greatly diminished capacity for phosphorylation of holo-pyruvate dehydrogenase complex(PDC), due to inability of transacetylase component of PDC to promote phosphorylation catalyzed by truncated PDK2. PMID: 16216081
    7. persistently elevated levels of circulating free fatty acid in normal and diabetic OLETF rats play an important role in stimulating PDK2 and PDK4 expression in liver PMID: 16483874
    8. Y80 and D117 are required for the communication between the dichloroacetate-binding site and active site of PDK2. PMID: 17544412
    9. Mobility of adenosine triphosphate (ATP) lid is central to allosteric regulation of pyruvate dehydrogenase kinase-2 activity serving as a conformational switch required for communication between active site and allosteric sites in the kinase molecule. PMID: 18627174

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  • 亚细胞定位：
    Mitochondrion matrix.
  • 蛋白家族：
    PDK/BCKDK protein kinase family
  • 组织特异性：
    Detected in heart (at protein level). Highest level of expression in heart and skeletal muscle and the lowest in spleen and lung. Liver, kidney, brain and testis levels are intermediate.
  • 数据库链接：

    KEGG: rno:81530

    UniGene: Rn.88597

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