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  4. Recombinant Rat [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial (Pdk4), partial, mitochondrial (Pdk4)

Recombinant Rat [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial (Pdk4), partial, mitochondrial (Pdk4)

  • 中文名称：
    大鼠Pdk4重组蛋白
  • 货号：
    CSB-YP017730RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大鼠Pdk4重组蛋白
  • 货号：
    CSB-EP017730RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大鼠Pdk4重组蛋白
  • 货号：
    CSB-EP017730RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大鼠Pdk4重组蛋白
  • 货号：
    CSB-BP017730RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大鼠Pdk4重组蛋白
  • 货号：
    CSB-MP017730RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Pdk4
  • Uniprot No.：
    O54937
  • 别名：
    Pdk4; [Pyruvate dehydrogenase; acetyl-transferring)] kinase isozyme 4; mitochondrial; EC 2.7.11.2; Pyruvate dehydrogenase kinase isoform 4
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    partial
  • 表达区域：
    -
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism in response to prolonged fasting and starvation. Plays an important role in maintaining normal blood glucose levels under starvation, and is involved in the insulin signaling cascade. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. In the fed state, mediates cellular responses to glucose levels and to a high-fat diet. Regulates both fatty acid oxidation and de novo fatty acid biosynthesis. Plays a role in the generation of reactive oxygen species. Protects detached epithelial cells against anoikis. Plays a role in cell proliferation via its role in regulating carbohydrate and fatty acid metabolism.
  • 基因功能参考文献：
    1. adipocyte PDK4 gene is a new target of the vitamin A derived retinoic acid and might participate to the reduced fatty acid efflux from the adipocyte PMID: 27981737
    2. C/EBPbeta stimulates Pdk4 expression and participates in the T(3) induction of the Cpt1a and Pdk4 genes. PMID: 21586575
    3. Report effect of phase delay lighting rotation schedule on daily expression of pdk4 in the heart and liver of Wistar rats. PMID: 21076970
    4. Data demonstrate an epinephrine-mediated regulation of PDK4 mRNA levels in white adipose tissue and suggest that p38 MAPK and PPARgamma could be involved in this pathway. PMID: 20739620
    5. Data demonstrate that glucocorticoids and insulin each modulate pyruvate dehydrogenase kinase 4 gene expression through complex hormone response units that contain multiple factors. PMID: 19703515
    6. we have identified two binding sites for thyroid hormone receptor beta in the promoter of the rat PDK4 (rPDK4) gene PMID: 19948729
    7. Evaluation of the role of peroxisome-proliferator-activated receptor alpha in the regulation of cardiac expression in response to starvation, high-fat feeding and hyperthyroidism PMID: 12049632
    8. upregulation of PDK4 in skeletal muscle PMID: 12099888
    9. In conclusion, the present data indicate that insulin had a profound effect to suppress PDK-4 expression in skeletal muscle and that, contrary to previous suggestions, circulating FFA had little impact on PDK-4 mRNA expression, at least within 5 h. PMID: 15026305
    10. impaired insulin's effect to suppress pyruvate dehydrogenase kinase (PDK) 4(PDK4) expression may explain the association between PDK4 overexpression and insulin resistance in skeletal muscle PMID: 16873695
    11. calcium signalling is involved in regulating the peroxisome proliferative activated receptor gamma coactivator 1 alpha and hexokinase II genes, but not pyruvate dehydrogenase kinase 4 PMID: 17516843
    12. Pyruvate dehydrogenase kinase-4 (PDK4) protein levels were higher in obese rats, and consequently pyruvate dehydrogenase complex activity was reduced PMID: 17957038
    13. cardiac-specific overexpression of PDK4 is sufficient to cause a loss of metabolic flexibility that exacerbates cardiomyopathy caused by the calcineurin stress-activated pathway PMID: 18083902
    14. PDK4 upregulation in adipocytes participates in the hypolipidemic effect of thiazolidinediones through modulation of glyceroneogenesis PMID: 18519799

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  • 亚细胞定位：
    Mitochondrion matrix.
  • 蛋白家族：
    PDK/BCKDK protein kinase family
  • 组织特异性：
    Ubiquitous; highest levels of expression in heart and skeletal muscle.
  • 数据库链接：

    KEGG: rno:89813

    STRING: 10116.ENSRNOP00000012759

    UniGene: Rn.11766

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