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  4. Recombinant Rat Histone H1t (Hist1h1t)

Recombinant Rat Histone H1t (Hist1h1t)

  • 中文名称：
    大鼠Hist1h1t重组蛋白
  • 货号：
    CSB-YP010383RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大鼠Hist1h1t重组蛋白
  • 货号：
    CSB-EP010383RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大鼠Hist1h1t重组蛋白
  • 货号：
    CSB-EP010383RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大鼠Hist1h1t重组蛋白
  • 货号：
    CSB-BP010383RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大鼠Hist1h1t重组蛋白
  • 货号：
    CSB-MP010383RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Hist1h1t
  • Uniprot No.：
    P06349
  • 别名：
    Hist1h1t; H1ft; H1t; Histone H1t
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-208
  • 氨基酸序列
    SETAPAASS TLVPAPVEKP ATKRRGKKPG MATARKPRGF SVSKLIPEAL SMSQERAGMS LAALKKALAA AGYDVEKNNS RIKLALKRLV NKGVLVQTKG TGASGSFKLS KKAASGNDKG KGKKSASAKA KKLGLSRASR SPKSSKTKVV KKPKATPTKG SGSRRKTKGA KGLQQRKSPA KARATNSNSG KSKMVMQKTD LRKAAGRK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Testis-specific histone H1 that forms less compacted chromatin compared to other H1 histone subtypes. Formation of more relaxed chromatin may be required to promote chromatin architecture required for proper chromosome regulation during meiosis, such as homologous recombination. Histones H1 act as linkers that bind to nucleosomes and compact polynucleosomes into a higher-order chromatin configuration.
  • 基因功能参考文献：
    1. studies suggest that Sp transcription factors may be involved in transcription of the histone H1t gene and the GC-box and the TE1 subelement are required for activation of the H1t promoter PMID: 12297531
    2. These data support the hypothesis that RFX2 expression is upregulated in spermatocytes where it participates in activating transcription of the H1t gene and other testis genes. PMID: 16676351
    3. The glutamine residue in histone H1t forms a hydrogen bond with main chain carbonyl of methionine-52 (in H1t) and is spatially oriented away from the nucleosome dyad axis. PMID: 17052712
    4. RFX2 and NF-Y binding to the testis-specific histone H1t promoter may be required for transcriptional activation in primary spermatocytes PMID: 18247329
  • 亚细胞定位：
    Nucleus. Chromosome.
  • 蛋白家族：
    Histone H1/H5 family
  • 组织特异性：
    Testis-specific. Expressed in pachytene spermatocytes during meiotic prophase I.
  • 数据库链接：

    KEGG: rno:24438

    UniGene: Rn.138104

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