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  4. Recombinant Rat Glutamate receptor ionotropic, NMDA 1 (Grin1)(F484A,T518A,R523A,W731A), partial-VLPs, Fluorescent

Recombinant Rat Glutamate receptor ionotropic, NMDA 1 (Grin1)(F484A,T518A,R523A,W731A), partial-VLPs, Fluorescent

  • 中文名称：
    Recombinant Rat Glutamate receptor ionotropic, NMDA 1 (Grin1)(F484A,T518A,R523A,W731A), partial-VLPs, Fluorescent
  • 货号：
    CSB-MP009911RA2(M)
  • 规格：
    ￥5250
  • 图片：
    • CSB-MP009911RA2(M) is detected by Mouse anti-GFP monoclonal antibody.
    • The purity of VLPs was greater than 95% as determined by SEC-HPLC
  • 其他：

产品详情

  • 纯度：
    Greater than 95% as determined by SEC-HPLC.
  • 生物活性：
    Not Test
  • 基因名：
    Grin1
  • Uniprot No.：
    P35439
  • 别名：
    GluN1;Glutamate [NMDA] receptor subunit zeta-1;N-methyl-D-aspartate receptor subunit NR1;NMD-R1
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Partial
  • 来源：
    Mammalian cell
  • 分子量：
    73.0 kDa
  • 表达区域：
    396-800aa(F484A,T518A,R523A,W731A)
  • 氨基酸序列
    TRLKIVTIHQEPFVYVKPTMSDGTCKEEFTVNGDPVKKVICTGPNDTSPGSPRHTVPQCCYGFCIDLLIKLARTMNFTYEVHLVADGKAGTQERVNNSNKKEWNGMMGELLSGQADMIVAPLAINNEAAQYIEFSKPFKYQGLTILVKKEIPRSTLDSFMQPFQSTLWLLVGLSVHVVAVMLYLLDRFSPFGRFKVNSEEEEEDALTLSSAMWFSWGVLLNSGIGEGAPRSFSARILGMVWAGFAMIIVASYTANLAAFLVLDRPEERITGINDPRLRNPSDKFIYATVKQSSVDIYFRRQVELSTMYRHMEKHNYESAAEAIQAVRDNKLHAFIADSAVLEFEASQKCDLVTTGELFFRSGFGIGMRKDSPWKQNVSLSILKSHENGFMEDLDKTWVRYQECDS
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    C-terminal EGFP-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein indeionized sterile water to a concentration of 0.1-1.0 mg/mL.Aliquot for long-term storage at -80℃. Solubilize for 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 注意事项：
    The VLPs are expressed from human 293 cells (HEK293).Mix the sample gently by repeatedly pipetting it up and down. Do not vortex.Repeated freezing and thawing is not recommended.Store the protein at -20℃/-80℃ upon receiving it, and ensure to avoid repeated freezing and thawing, otherwise, it will affect the protein activity. The immunization strategy should be optimized (antigen dose, regimen and adjuvant).
  • Datasheet & COA：
    Please contact us to get it.

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Grin1 Proteins

靶点详情

  • 功能：
    Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+). Sensitivity to glutamate and channel kinetics depend on the subunit composition.
  • 基因功能参考文献：
    1. Social isolation rearing of rats throughout adolescence results in decreased density of GluN1 in dopamine-receptive dendrites of the nucleus accumbens core. PMID: 29779156
    2. The relative gene expression of rostral ventral lateral medulla (RVLM) NMDA and AMPA glutamate-gated receptor subunits and protein concentration of select receptor subunits did not differ between young and aged rats, and there were no age-related differences in the expression of RVLM ionotropic GABAA and Gly receptors, or of protein concentration of select GABAA subunits. PMID: 28263869
    3. NMDAR1 is significantly activated in hypothalamic paraventricular nucleus in rats with heart failure. PMID: 28958939
    4. Study demonstrates that in the rat model, endometriosis can be associated with a decrease in mu opioid receptors (MOR)immunoreactivity within neuronal compartments in addition to a shift in MOR and N-methyl-d-aspartate subunit receptor (NR1) receptor expression within the ventral periaqueductal gray. PMID: 27089914
    5. restoring chloride homeostasis normalizes both presynaptic and postsynaptic NMDAR activity increased by nerve injury in the spinal dorsal horn. PMID: 27160909
    6. tPA is a ligand of the N-terminal domain of the obligatory GluN1 subunit of NMDAR acting as a modulator of their dynamic distribution at the neuronal surface and subsequent signaling. PMID: 27831563
    7. Allosteric interactions occur between the glutamate-binding GluN2 subunits in triheteromeric GluN1/2A/2B NMDARs. This allosterism is dominated by the GluN2A subunit and results in functional properties not predicted by those of diheteromeric GluN1/2A and GluN1/2B NMDARs. PMID: 28384476
    8. Single unilateral infusion of NMDA at mild dose into the hippocampal formation induced chronic, focal neurodegeneration accompanied by severe and progressive neuroinflammation and affected preferentially principal neurons; GluN1 and GluN2B subunits of NMDA receptor were significantly upregulated up to 3 days after the NMDA infusion, while GABAA alpha5 subunit was downregulated at 30 days after the lesion PMID: 26930443
    9. In neuronal cell line (NG108-15) in vitro, hypoxia caused a significant increase in mRNA and protein for NMDA-NR1 mRNA and protein levels. PMID: 27810863
    10. Interferon gamma (IFNgamma) enhance the amplitude of NMDA-induced inward currents in substantia gelatinosa neurons via microglial IFNgamma receptors and CCL2/CCR2 signaling. PMID: 27094552
    11. shRNA-mediated knockdown of Shank3 results in up-regulation of the NMDA receptor subunit GluN1 in axon terminals. PMID: 26725465
    12. young adult runners demonstrated a significant increase in total GluN (1 and 2A) subunit expression in the dorsal hippocampus, and an opposing effect in the ventral hippocampus. PMID: 26220171
    13. NMDA receptor hyperfunction in lipopolysaccharide-treated kidneys was demonstrated by NR1 upregulation, particularly in renal tubules. IL-1beta-mediated NMDA receptor hyperfunction participates in lipopolysaccharide-induced tubular damage. PMID: 26133372
    14. This study demonstrated that the Male prenatal chronic mild stress rats expressed significantly lower NR1 levels in the prefrontal cortex. and lower in femal rat hippocampus. PMID: 26071959
    15. Hepatic encephalopathy affects the gene expression of Grin1 in brain with a site-specific pattern. PMID: 25896221
    16. Remifentanil induces an increase in GluN1 mRNA and protein levels in primary rat dorsal horn neurons, which may partially account for remifentanil-induced hyperalgesia. PMID: 25867330
    17. Contacts specific to GluN1 and GluN2A NMDA receptors, which brace the heterodimer interface within the ligand binding domain, stabilize actively gating receptor conformations and result in longer bursts and slower deactivations. PMID: 26370091
    18. MARK/Par1 is activated downstream of NMDA receptors in primary hippocampal neurons. PMID: 25932647
    19. Dorsal hippocampal receptor complex levels containing NR1/GluA1/GluA2 were modulated by long term potentiation induction. PMID: 24442866
    20. Long-term potentiation (LTP) and long-term depression (LTD) signaling converge towards phosphatidylinositol-(3,4,5)-trisphosphate (PIP3) upregulation, but PTEN acts as an LTD-selective switch that determines the outcome of PIP3 accumulation. PMID: 25335889
    21. we found that NMDARs are released from the ER only when two asparagine residues in the GluN1 subunit (Asn-203 and Asn-368) are N-glycosylated PMID: 26045554
    22. Long-term blockade of the NMDA receptor in developing rat hippocampal neurons significantly increased NR1 subunit expression, and that this was associated with an alteration in neuronal activity. PMID: 25937004
    23. KN-62, a selective inhibitor of rat brain Ca(2+)/calmodulin-dependent protein kinase II (CaMKII), diminished the increase in serine phosphorylation of GluR6. PMID: 16126302
    24. GluN1 amino-terminal domain adopts a more open conformation when coassembled with GluN2A than with GluN2B. PMID: 25829490
    25. Data show that the receptor tyrosine kinase-like orphan receptor 2 (RoR2) functions as a Wnt5a receptor required to maintain basal NMDA receptors (NMDARs)-mediated synaptic transmission. PMID: 25825749
    26. In summary, we identified genes that are downregulated in the PFC of SHR, and might be related to the behavioral abnormalities exhibited by this strain. PMID: 25041985
    27. NMDAR1 were increased in the prefrontal cortex (PC), hippocampus and amygdala of CHF, and decreased in the hippocampus, amygdala and NAc of CHF stressed. PMID: 25772108
    28. The ligand binding domains (LBD) of the GluN1 and GluN2A subunits of the NMDAR heterodimerize only when both coagonists, Glu and Gly/d-Ser, bind to their respective sites on GluN2 and GluN1. PMID: 25544544
    29. acute 5-HT7 receptor activation increases NR1 NMDA receptor subunit phosphorylation and differentially alters the phosphorylation state of the NR2B and NR2A subunits. PMID: 23672716
    30. The hippocampus of fetuses gestated under light:dark photoperiod (12:12 LD) displayed daily oscillatory expression of the clock genes Bmal1 and Per2, clock-controlled genes Mtnr1b, Slc2a4, Nr3c1 and NMDA receptor subunits 1B-3A-3B PMID: 24663672
    31. Redox regulation of NMDAR synaptic responses are reglated by antoxidant enzymes,SOD1. PMID: 24388786
    32. Antibody-mediated downregulation of surface NMDARs engages homeostatic synaptic plasticity mechanisms, which may inadvertently contribute to immune-mediated encephalitis progression. PMID: 24916964
    33. NR1 protein expression in the rat retina by immunofluorescence double labeling: the NR1 subunit is expressed more widely than was previously appreciated PMID: 22512920
    34. data support the argument that PS to pregnant dams could induce depression-like behavior, which may be involved with abnormal expression of NR1 and NR2A in specific brain regions, and MK-801 may have antidepressant-like effects on the juvenile offspring PMID: 24278457
    35. The results indicate that NMDAR1 and CREB are involved in the ocular dominance forming process and play a role in vision plasticity PMID: 23381906
    36. alpha7nAchR/NMDAR coupling may selectively affect some aspects of learning and memory. PMID: 24360204
    37. A single amino acid in GluN1 that is critical for priming of NMDA receptors by glycine, was identified. PMID: 23941530
    38. The altered NR1 could have potential impact on anxiety-like behavior in the adult offspring exposed to Prenatal stress. PMID: 24029697
    39. this study shows the crystal structure of the intact heterotetrameric GluN1-GluN2B NMDA receptor ion channel at 4 angstroms. PMID: 24876489
    40. Crystallization and structural analysis of the GluN1 and GluN3A ligand binding domains of the rat NMDA receptor subunits Grin1 and Grin3a. PMID: 23972471
    41. We developed a method to provide selective cell-surface expression of recombinant GluN1/GluN2A/GluN2B triheteromers and compared properties of these receptors with those of GluN1/GluN2A and GluN1/GluN2B diheteromers. PMID: 24607230
    42. Virtually all bulbospinal neurons in the rostral ventrolateral medulla express NR1. PMID: 23562375
    43. Exercise training decreases persistent postsurgical pain via decreased expression of spinal cord NR1, TNF-alpha, and IL-6. PMID: 23640243
    44. Crystal structure and pharmacological characterization of a novel N-methyl-D-aspartate (NMDA) receptor antagonist at the GluN1 glycine binding site. PMID: 24072709
    45. Data from molecular modeling suggest that GluN1-containing NMDA receptors can enter a conformation or mode that allows the pore to gate with high probability of burst of activity. PMID: 23578394
    46. NR1 mRNA expression increased in the hippocampus in a model of chronic temporal lobe epilepsy. PMID: 23392471
    47. Data indicate that AMPA receptors have an alternating subunit arrangement of GluA1/GluA2, whereas NMDA receptors adopt an adjacent arrangement of GluN1/GluN2A, whereas NMDA receptors adopt an adjacent arrangement. PMID: 23760273
    48. Feeding-specific circuits between the nucleus accumbens shell and the lateral hypothalamus are regulated by NMDA receptors (and GABA(A) receptors). PMID: 23164617
    49. Findings suggest a mechanism in which NMDAR-dependent phosphorylation of ERK1/2 modulates satiation signals via synapsin I phosphorylation in vagal afferent endings. PMID: 23715865
    50. GluN1, an NMDA receptor subunit in the adult rat hippocampus undergoes similar changes after 5 minutes in an open field and after long term potentiation-induction. PMID: 23383317

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane. Cell junction, synapse, postsynaptic density.
  • 蛋白家族：
    Glutamate-gated ion channel (TC 1.A.10.1) family, NR1/GRIN1 subfamily
  • 组织特异性：
    Detected throughout the brain, in brain cortex, cerebellum, thalamus and olfactory bulb.
  • 数据库链接：

    KEGG: rno:24408

    STRING: 10116.ENSRNOP00000029227

    UniGene: Rn.9840

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