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  4. Recombinant Rat Focal adhesion kinase 1 (Ptk2), partial

Recombinant Rat Focal adhesion kinase 1 (Ptk2), partial

  • 中文名称：
    大鼠Ptk2重组蛋白
  • 货号：
    CSB-YP018993RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大鼠Ptk2重组蛋白
  • 货号：
    CSB-EP018993RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大鼠Ptk2重组蛋白
  • 货号：
    CSB-EP018993RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大鼠Ptk2重组蛋白
  • 货号：
    CSB-BP018993RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大鼠Ptk2重组蛋白
  • 货号：
    CSB-MP018993RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Ptk2
  • Uniprot No.：
    O35346
  • 别名：
    Ptk2; Fak; Fak1Focal adhesion kinase 1; FADK 1; EC 2.7.10.2; Focal adhesion kinase-related nonkinase; FRNK; Protein-tyrosine kinase 2; p125FAK; pp125FAK
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Non-receptor protein-tyrosine kinase that plays an essential role in regulating cell migration, adhesion, spreading, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, cell cycle progression, cell proliferation and apoptosis. Required for early embryonic development and placenta development. Required for embryonic angiogenesis, normal cardiomyocyte migration and proliferation, and normal heart development. Regulates axon growth and neuronal cell migration, axon branching and synapse formation; required for normal development of the nervous system. Plays a role in osteogenesis and differentiation of osteoblasts. Functions in integrin signal transduction, but also in signaling downstream of numerous growth factor receptors, G-protein coupled receptors (GPCR), EPHA2, netrin receptors and LDL receptors. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascade. Promotes activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling cascade. Promotes localized and transient activation of guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs), and thereby modulates the activity of Rho family GTPases. Signaling via CAS family members mediates activation of RAC1. Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Phosphorylates SRC; this increases SRC kinase activity. Phosphorylates ACTN1, ARHGEF7, GRB7, RET and WASL. Promotes phosphorylation of PXN and STAT1; most likely PXN and STAT1 are phosphorylated by a SRC family kinase that is recruited to autophosphorylated PTK2/FAK1, rather than by PTK2/FAK1 itself. Promotes phosphorylation of BCAR1; GIT2 and SHC1; this requires both SRC and PTK2/FAK1. Promotes phosphorylation of BMX and PIK3R1.; Does not contain a kinase domain and inhibits PTK2/FAK1 phosphorylation and signaling. Its enhanced expression can attenuate the nuclear accumulation of LPXN and limit its ability to enhance serum response factor (SRF)-dependent gene transcription.
  • 基因功能参考文献：
    1. Study demonstrated that MIF overexpression promotes the proliferation and migration of ASMCs by upregulating the activity of the ERK1/2 and FAK signaling pathways in these cells. PMID: 28851074
    2. thrombin stimulation of retinal pigment epithelium cell transformation and migration are regulated by FAK tyrosine phosphorylation. PMID: 28445805
    3. Stimulation of glucose transport in cardiomyocytes requires FAK activity prior to stimulation. The chronic reduction of FAK activity in cardiomyocytes exposed to free fatty acids contributes to the loss of glucose transport responsiveness to insulin or metabolic inhibition. PMID: 27428469
    4. In cardiomyocytes exposed to biomechanical stimulation, FAK accumulates in the nucleus, binds to and upregulates the transcriptional activity of MEF2c through an interaction with the FAK focal adhesion targeting (FAT) domain. PMID: 27427476
    5. TGF-beta1-induced epithelial-myofibroblast plasticity is FAK- dependent, whereas TGF-beta1-induced apoptosis is favored when FAK signaling is inhibited. PMID: 28360109
    6. Pre-treatment of p38 inhibitor did not alter FAK activation. PMID: 27704688
    7. RACK1 promotes adhesion-mediated activation of ERK2 that in turn inhibits p190A-RhoGAP signaling by reducing the peripheral localization of p190A-RhoGAP. PMID: 27212270
    8. Hydrostatic mechanical force enhances the viability and decreases the apoptosis of condylar chondrocytes through integrin-FAK-ERK/PI3K pathway. PMID: 27827993
    9. The objectives of this study are to assess the localization of FAK phosphorylated at tyrosine residues in the sinus endothelial cells of rat spleen. PMID: 26846226
    10. Spinal cord injury results in diminished FAK signaling and is independent of ERK1/2 and Akt protein. PMID: 26481700
    11. MGF-C25E promotes tenocyte migration by increasing nuclear stiffness via the FAK-ERK1/2 signaling pathway. PMID: 26742689
    12. Synovial FAK expression was positive in collagen-induced arthritis rats, indicating that it played an important role in the pathogenesis of RA. DMARDs could reduce the FAK expression in synovial cells of CIA rats. PMID: 26884826
    13. Divergent modulation of Rho-kinase and Ca(2+) influx pathways by Src family kinases and focal adhesion kinase in airway smooth muscle PMID: 26294392
    14. Dats show that CXCL10 chemokine treatment promoted the activation of p38 MAPK pathway through CXCR3 receptor signaling, thereby up-regulating focal adhesion kinase (FAK) activation and promoted cardiac microvascular endothelial cell migration. PMID: 26835911
    15. Fak protein plays role in cellular proliferation in tendon fibroblasts PMID: 25898827
    16. Inhibition of the early step of Focal adhesion kinase and proline-rich tyrosine kinase activation may comprise an important portion of the response expressed by modulation of some coupled signal transduction pathways. PMID: 25708150
    17. these results demonstrate that FAK and Rac1 signalings coordinately regulate the dynamics of FAs during VEGF-induced migration of MSCs in varying neural differentiation states. PMID: 25820249
    18. The present study was designed to investigate whether FAK/Pyk2 and ERK1/2 MAPK signaling pathways participate in Perivascular adipocyte functions, which is activated by insulin-like growth factor 1(IGF-1) and inhibited by Gax. PMID: 25280940
    19. Correlation analysis revealed that integrin-alpha expression or FAK phosphorylation was positively associated with cytosolic CRT expression. PMID: 24930861
    20. LIPUS may affect the integrin-FAK-PI3K/Akt mechanochemical transduction pathway and alter ECM production by OA chondrocytes. PMID: 24742749
    21. both alphanu and beta1 integrins interfered with Abeta-induced neurotoxicity in hippocampal neurons and that this mechanism partially contributes to the activation of the Integrin-FAK signaling pathway. PMID: 23755149
    22. We established that phosphorylation of FAK Y397 with association with beta1 and beta3 integrins occurs with pressure-induced eutrophic remodeling. PMID: 25300309
    23. RACK1 scaffolds AGAP2 to FAK to regulate FAK activity and cell adhesion during the differentiation process. PMID: 24056044
    24. The key step for activating FAK's kinase-dependent functions--autophosphorylation of tyrosine-397--requires site-specific dimerization via association of its N-terminal FERM domain, stabilized by interaction between FERM and the C-terminal FAT domain. PMID: 24480479
    25. p-FAK-Tyr(407) has a role in affecting F-actin organization after perfluorooctanesulfonate-induced perturbation of male rat Sertoli cell blood-testis barrier function PMID: 24169556
    26. These findings illustrate a mechanistic pathway mediated by p-FAK-Tyr(397) that regulates spermatid adhesion at the apical ectoplasmic specialization in vivo. PMID: 23880313
    27. The results highlight that the expression of components for costameric attachment sites of myofibrils is under load- and fiber type-related control via FAK and its inhibitor FRNK. PMID: 23904105
    28. Cadmium affects focal adhesion kinase (FAK) in mesangial cells: involvement of CaMK-II and the actin cytoskeleton. PMID: 23463649
    29. neuregulin successfully improves axo-glial interaction by speeding Sc migration via the erbB2/3-FAK pathway PMID: 23301073
    30. Suggest that FAK acts as a central coordinator of integrin and growth factor-mediated S-phase entry by regulating Cdk2 in EGF-stimulated hepatocytes. PMID: 23168795
    31. critical role of sFRP1 in regulating spermiation via its effects on the FAK signaling and retention of nectin-3 adhesion complex at the apical ectoplasmic specialization PMID: 23073828
    32. The extracellular activation of matrix metalloproteinase 2 accelerated collagen-I synthesis in cardiac fibroblasts. Focal adhesion kinase phosphorylation (Tyr397) played a pivotal role in matrix metalloproteinase 2-stimulated collagen-I synthesis. PMID: 22914642
    33. Thioredoxin reductase linked to cytoskeleton by focal adhesion kinase reverses actin S-nitrosylation and restores neutrophil beta(2) integrin function. PMID: 22778269
    34. FAK-Tyr(407) phosphorylation promotes BTB integrity by strengthening the actin filament-based cytoskeleton. PMID: 22797892
    35. Angiotensin (Ang)II)-immunocomplexes in postnatal day-15 rat hindbrain contain mainly the 85 kDa fragment of FAK; besides, p125FAK associates to SHP-1. PMID: 22120166
    36. FAK silencing was protective against lung collagen deposition underscores the therapeutic potential of FAK targeting by small interfering RNA. PMID: 22293597
    37. FAK is activated in endotoxemia, playing a role in cardiac remodeling and in the impairment of cardiac function. PMID: 21921830
    38. Findings show that P-glycoprotein (Abcb1a and Abcb1b) structurally interacted with focal adhesion kinase (FAK), creating the occludin/ZO-1/FAK/P-glycoprotein regulatory complex. PMID: 22106313
    39. Salvia miltiorrhiza monomer IH764-3 can induce the expression of MMP-13 and inhibit the expression of TIMP-1 by down-regulating the expression of FAK. PMID: 21180139
    40. FAK undergoes S910 phosphorylation via PKCdelta and Src-dependent pathways that are important for cell spreading and sarcomere reorganization in cardiac hypertrophy. PMID: 21937583
    41. FRNK inhibits both FAK and proline-rich tyrosine kinase 2 (PYK2) in cultured vascular smooth muscle cells (VSMCs), and both kinases may be involved in VSMC invasion during vascular remodeling. PMID: 21852560
    42. Role of TINag in cell survival utilizing integrin (alpha)vbeta3/focal adhesion kinase (FAK)/phosphatidylinositol 3-kinase (PI3K)/protein kinase B-serine/threonine kinase (AKT) signaling pathway. PMID: 21795690
    43. Hypoxic preconditioning enhances the ability of mesenchymal stem cell migration; this effect is mediated through a regulatory role of Kv2.1 on FAK phosphorylation/activation. PMID: 21562308
    44. These results suggest that the integrin-associated tyrosine kinase FAK contributes to the regulation of the osteoblast differentiation in part through the regulation of Cbfa1 expression. PMID: 21412826
    45. These data show that FAK regulates HSC proliferation and induces the apoptosis of HSC via the caspase-3 and Bcl-2/Bax pathway. PMID: 21569089
    46. Rac1 through NADPH oxidase is part of the signaling pathway constituted by FAK, Rac1, and ERK that regulates focal adhesion disassembly during cell spreading. PMID: 21660950
    47. cell surface glycocalyx HSPGs, in the presence of integrin-mediated cell-matrix adhesions and cytoskeleton organization, sense interstitial flow and activate the FAK-ERK signaling axis, leading to upregulation of MMP expression and cell motility PMID: 21246051
    48. Phosphorylation of FAK and PYK2 is upregulated by elevated blood pressure but not by humoral factors in the rat aorta, demonstrating novel aspects of atherogenesis in hypertension. PMID: 21068519
    49. FRNK has independent signaling functions in vascular smooth muscle cells(decreased migratory capacity,decreased proliferation,etc.) PMID: 20705914
    50. FAK gene silencing suppresses adhesion and migration of hepatic stellate cells. PMID: 19912685

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  • 亚细胞定位：
    Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, perinuclear region. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus. Cytoplasm, cytoskeleton, cilium basal body.
  • 蛋白家族：
    Protein kinase superfamily, Tyr protein kinase family, FAK subfamily
  • 数据库链接：

    KEGG: rno:25614

    STRING: 10116.ENSRNOP00000011219

    UniGene: Rn.2809

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