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  4. Recombinant Rat Cytoplasmic aconitate hydratase (Aco1), partial

Recombinant Rat Cytoplasmic aconitate hydratase (Aco1), partial

  • 中文名称：
    Recombinant Rat Cytoplasmic aconitate hydratase(Aco1) ,partial
  • 货号：
    CSB-YP737180RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Rat Cytoplasmic aconitate hydratase(Aco1) ,partial
  • 货号：
    CSB-EP737180RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Rat Cytoplasmic aconitate hydratase(Aco1) ,partial
  • 货号：
    CSB-EP737180RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Rat Cytoplasmic aconitate hydratase(Aco1) ,partial
  • 货号：
    CSB-BP737180RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Rat Cytoplasmic aconitate hydratase(Aco1) ,partial
  • 货号：
    CSB-MP737180RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Aco1
  • Uniprot No.：
    Q63270
  • 别名：
    Aco1; Ireb1; Irebp; Cytoplasmic aconitate hydratase; Aconitase; EC 4.2.1.3; Citrate hydro-lyase; Iron regulatory protein 1; IRP1; Iron-responsive element-binding protein 1; IRE-BP 1
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Bifunctional iron sensor that switches between 2 activities depending on iron availability. Iron deprivation, promotes its mRNA binding activity through which it regulates the expression of genes involved in iron uptake, sequestration and utilization. Binds to iron-responsive elements (IRES) in the untranslated region of target mRNAs preventing for instance the translation of ferritin and aminolevulinic acid synthase and stabilizing the transferrin receptor mRNA.; Conversely, when cellular iron levels are high, binds a 4Fe-4S cluster which precludes RNA binding activity and promotes the aconitase activity, the isomerization of citrate to isocitrate via cis-aconitate.
  • 基因功能参考文献：
    1. Induction of aconitate hydratase in hepatocytes of starving rats. PMID: 12139479
    2. Proteomic analysis revealed that the expression levels of two mitochondrial proteins, cytochrome bc1 complex2 (UQCRC2, the major component of electron transport chain complex III) and aconitase (an enzyme involved in the tricarboxylic acid cycle), were decreased by homocysteine in neural stem cells PMID: 27914013
    3. Iron increases turnover of IRP-1 in kidney cells, while increasing aconitase activity, suggesting that the apoprotein (aconitase-inactive) form is not exclusively responsible for turnover. PMID: 25652229
    4. These results indicate that aconitase can exert control over respiration (with tissue specificity) and support the hypothesis that inactivation of aconitase may provide a control mechanism to prevent O2(-) and H2O2 formation by the respiratory chain. PMID: 24601712
    5. Polymer-dependent translocation of IRP-1 in Cd(2+)-exposed cells may underlie effects of Cd(2+) on iron homeostasis. PMID: 25106854
    6. Human ACO1 is a moonlighting protein that has both aconitase enzyme activity as well as an mRNA binding function. PMID: 8041788
    7. Results suggest that IL-1beta and TNF-alpha released by microglia might contribute to iron accumulation in ventral mesencephalic neurons by upregulating IRP1 and hepcidin levels through reactive oxygen/nitrogen species production. PMID: 23376588
    8. The RNA-binding and aconitase forms of IRP-1 can undergo interconversion dependent on the density of cells growing in culture. PMID: 22544036
    9. c-aconitase is a critical target involved in the assimilation of Fe and excess dietary Zn can result in negative interactions PMID: 22900282
    10. Both posttranslational and posttranscriptional regulation of IRE-BP may take part in iron metabolism balance. PMID: 15625891
    11. Results indicate that iron-regulatory-protein 1-binding activity in the membrane fraction can be altered by cell stress or iron status and that phosphorylation plays a role in the translocation. PMID: 16144863
    12. Zinc and iron interact negatively with cytosolic aconitase. PMID: 16198227
    13. decreased activity of aconitase contributes to the endotoxin-stimulated reduction in mitochondrial respiration PMID: 18005658

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  • 亚细胞定位：
    Cytoplasm, cytosol.
  • 蛋白家族：
    Aconitase/IPM isomerase family
  • 数据库链接：

    KEGG: rno:50655

    STRING: 10116.ENSRNOP00000008337

    UniGene: Rn.35934

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