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  4. Recombinant Rat Cytochrome P450 1A2 (Cyp1a2)

Recombinant Rat Cytochrome P450 1A2 (Cyp1a2)

  • 中文名称：
    Recombinant Rat Cytochrome P450 1A2(Cyp1a2)
  • 货号：
    CSB-YP006396RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Rat Cytochrome P450 1A2(Cyp1a2)
  • 货号：
    CSB-EP006396RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Rat Cytochrome P450 1A2(Cyp1a2)
  • 货号：
    CSB-EP006396RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Rat Cytochrome P450 1A2(Cyp1a2)
  • 货号：
    CSB-BP006396RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Rat Cytochrome P450 1A2(Cyp1a2)
  • 货号：
    CSB-MP006396RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Cyp1a2
  • Uniprot No.：
    P04799
  • 别名：
    Cyp1a2; Cyp1a-2Cytochrome P450 1A2; EC 1.14.14.1; CYPIA2; Cholesterol 25-hydroxylase; Cytochrome P-448; Cytochrome P-450d; Cytochrome P450-D; Hydroperoxy icosatetraenoate dehydratase; EC 4.2.1.152
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-513
  • 氨基酸序列
    AFSQYISLA PELLLATAIF CLVFWVLRGT RTQVPKGLKS PPGPWGLPFI GHMLTLGKNP HLSLTKLSQQ YGDVLQIRIG STPVVVLSGL NTIKQALVKQ GDDFKGRPDL YSFTLITNGK SMTFNPDSGP VWAARRRLAQ DALKSFSIAS DPTSVSSCYL EEHVSKEANH LISKFQKLMA EVGHFEPVNQ VVESVANVIG AMCFGKNFPR KSEEMLNLVK SSKDFVENVT SGNAVDFFPV LRYLPNPALK RFKNFNDNFV LFLQKTVQEH YQDFNKNSIQ DITGALFKHS ENYKDNGGLI PQEKIVNIVN DIFGAGFETV TTAIFWSILL LVTEPKVQRK IHEELDTVIG RDRQPRLSDR PQLPYLEAFI LEIYRYTSFV PFTIPHSTTR DTSLNGFHIP KECCIFINQW QVNHDEKQWK DPFVFRPERF LTNDNTAIDK TLSEKVMLFG LGKRRCIGEI PAKWEVFLFL AILLHQLEFT VPPGVKVDLT PSYGLTMKPR TCEHVQAWPR FSK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2-hydroxy E1 and E2. Metabolizes cholesterol toward 25-hydroxycholesterol, a physiological regulator of cellular cholesterol homeostasis. May act as a major enzyme for all-trans retinoic acid biosynthesis in the liver. Catalyzes two successive oxidative transformation of all-trans retinol to all-trans retinal and then to the active form all-trans retinoic acid. Primarily catalyzes stereoselective epoxidation of the last double bond of polyunsaturated fatty acids (PUFA), displaying a strong preference for the (R,S) stereoisomer. Catalyzes bisallylic hydroxylation and omega-1 hydroxylation of PUFA. May also participate in eicosanoids metabolism by converting hydroperoxide species into oxo metabolites (lipoxygenase-like reaction, NADPH-independent). Plays a role in the oxidative metabolism of xenobiotics. Catalyzes the N-hydroxylation of heterocyclic amines and the O-deethylation of phenacetin. Metabolizes caffeine via N3-demethylation.
  • 基因功能参考文献：
    1. acute Formaldehyde (FA) poisoning suppressed the activities of CYP2C11, CYP2E1, and CYP3A2 and induced the activity of CYP1A2 in rats. And the change of CYP450 activity caused by acute FA poisoning may be associated with FA potential carcinogenic effects on the airways. PMID: 28555194
    2. Blast-induced traumatic brain injury has a significant impact on the expression of CYP1A2 in liver. PMID: 26913515
    3. CYP1A2 activity was reduced in rats after acute exposure to high altitude of 4300m and metronidazole. PMID: 27899252
    4. Intragastric administration of suberoylanilide hydroxamic acid inhibited CYP1A2 activity. PMID: 26191268
    5. As for CYP1A2 and CYP3A4, the negligible inhibitions were observed. PMID: 25764964
    6. Schizonepetin induced the expression of CYP3A1, CYP1A2 and CYP2E1 at dosages of 24 and 48 mg/kg. PMID: 23235327
    7. In vivo evaluation of the metabolic ratio of CYP2C9 and CYP1A2 drug markers after administration of afobazole in comparison to standard inducers and inhibitors of cytochromes PMID: 24555232
    8. no changes in the activity of CYP2C9 under the special environment of acute high altitude hypoxia PMID: 22512029
    9. Panax notoginseng saponins had no significant effects on the activities of CYP2C9, CYP2D6, and CYP3A4, but it could induce the activity of CYP1A2 in rats in vivo. PMID: 22162298
    10. Ephedra water decoction and maxing cough tablets (MXCT) pretreatment induced CYP1A2 activity. PMID: 21796703
    11. Tanshinone I up-regulates CYP1A2 mRNA expression in rat hepatocytes. PMID: 21899995
    12. data demonstrate that pregnancy decreases CYP1A2 activity and expression with a modest effect on CYP1A2 mRNA PMID: 20921303
    13. HO-1 expression was associated with lower CYP1A2 activity and lower NADPH-dependent reactive oxygen species production by rat liver microsomes. PMID: 20942796
    14. DNA damage measured as SB and DNA adducts was detectable in V79 r1A2-NH cells expressing rat CYP1A2 when treated with IQ (2.5-50 microM) and this was inhibited by quercetin. PMID: 11752233
    15. cigarette smoke increases CYP1A2 expression during rat hepatocarcinogenesis in rat PMID: 11802804
    16. Influence of acetaminophen and trichloroethylene on liver cytochrome P450-dependent monooxygenase system PMID: 11996102
    17. Hepatic CYP1A2 increased sharply just before weaning. There was a 4-fold decrease in enzyme expression down to adult levels by day 60. PMID: 12162855
    18. Cyp1a2 is upregulated after treatment with Aroclor in rat liver PMID: 12396271
    19. These results indicate that Kupffer cell-derived proinflammatory cytokines may play an important role in downregulating CYP1A2 in sepsis. PMID: 16820944
    20. Hepatic CYP1A2, CYP2C11, and PPAR-gamma were down-regulated after the initial stress (hemorrhage) PMID: 17101152
    21. CYP2E1 mRNA expression was low at birth, then increased rapidly to peak prior to weaning. CYP1A2 transcript levels remained very low postnatally and then increased dramatically to reach peak expression during weaning. PMID: 17366540
    22. Examine age-related changes in liver CYP1A2 and discuss role in modeling to predict human risk assessment. PMID: 17881659
    23. Tenofovir and its prodrug tenofovir disoproxil fumarate decreased liver microsmal CYP1A2 content. PMID: 17949244
    24. Stress is a significant factor in the regulation of the CYP1A2 inducibility by benzo(alpha)pyrene. PMID: 17973897
    25. alterations at AhR binding sites may also contribute to the downregulated CYP1A2 expression in sepsis PMID: 18360687
    26. Pharmacologic profiling of Cyp1a2 induction and competition is reported. PMID: 18493746
    27. Report effect of hemodialysis on hepatic cytochrome CYP1a2 functional expression. PMID: 18845914
    28. Faster clearance of omeprazole in mutant Nagase analbuminemic rats: possible roles of increased protein expression of hepatic CYP1A2 and lower plasma protein binding. PMID: 19288444

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  • 亚细胞定位：
    Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
  • 蛋白家族：
    Cytochrome P450 family
  • 数据库链接：

    KEGG: rno:24297

    STRING: 10116.ENSRNOP00000021653

    UniGene: Rn.5563

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