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  4. Recombinant Pseudomonas syringae pv. tomato Effector protein HopAB2 (hopAB2), partial

Recombinant Pseudomonas syringae pv. tomato Effector protein HopAB2 (hopAB2), partial

  • 中文名称：
    紫丁香假单胞菌番茄hopAB2重组蛋白
  • 货号：
    CSB-YP854628FGP
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    紫丁香假单胞菌番茄hopAB2重组蛋白
  • 货号：
    CSB-EP854628FGP
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    紫丁香假单胞菌番茄hopAB2重组蛋白
  • 货号：
    CSB-EP854628FGP-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    紫丁香假单胞菌番茄hopAB2重组蛋白
  • 货号：
    CSB-BP854628FGP
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    紫丁香假单胞菌番茄hopAB2重组蛋白
  • 货号：
    CSB-MP854628FGP
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    hopAB2
  • Uniprot No.：
    Q8RSY1
  • 别名：
    hopAB2; avrPtoB; PSPTO_3087; Effector protein HopAB2; Avirulence protein AvrPtoB) [Includes: E3 ubiquitin-protein ligase; EC 2.3.2.-; E3 ubiquitin-protein transferase)]
  • 种属：
    Pseudomonas syringae pv. tomato (strain DC3000)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Effector protein involved in gene-for-gene resistance in tomato plants. It is recognized by the host Pto resistance protein and elicits Pto and Prf-dependent hypersensitive response (HR) and programmed cell death (PCD), resulting in host immunity. In susceptible plants, acts as a virulence factor by suppressing PCD and HR-based plant immunity. This function requires its E3 ubiquitin ligase activity probably by recruiting E2 enzymes and transferring ubiquitin molecules to cellular proteins involved in regulation of PCD and targeting them for degradation. Also, induces expression of host genes involved in ethylene biosynthesis and signaling, in particular ACO1 and ACO2, encoding the ethylene-forming enzyme ACC oxidase.
  • 基因功能参考文献：
    1. This work uncovers a strategy in which Pseudomonas syringae AvrPtoB targets Arabidopsis NPR1 and represses NPR1-dependent salicylic acid signaling, thereby subverting plant innate immunity. PMID: 29174403
    2. AvrPto and AvrPtoB have phenotypically redundant avirulence activity on Pto-expressing tomato and additive virulence activities on susceptible tomato plants. PMID: 15672817
    3. we propose that both domains of Pseudomonas syringae pv tomato DC3000 AvrPtoB act together to support the virulence of PtoDC3000 in Arabidopsis through their ability to eliminate FLS2 from the cell periphery. PMID: 19062288
  • 亚细胞定位：
    Secreted. Note=Secreted via type III secretion system (TTSS). Localized to the plant cell cytoplasm.
  • 蛋白家族：
    HopAB family
  • 数据库链接：

    KEGG: pst:PSPTO_3087

    STRING: 223283.PSPTO_3087

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