Recombinant Pseudomonas aeruginosa Type IV major pilin protein PilA (pilA), partial

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中文名称：

铜绿假单胞菌pilA重组蛋白
货号：

CSB-EP361358EZX
规格：

￥2328
图片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

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纯度：

Greater than 85% as determined by SDS-PAGE.
基因名：

pilA
Uniprot No.：

P04739
种属：

Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
蛋白长度：

Partial
来源：

E.coli
分子量：

20.1 kDa
表达区域：

28-149aa
氨基酸序列

PQYQNYVARSEGASALATINPLKTTVEESLSRGIAGSKIKIGTTASTATETYVGVEPDANKLGVIAVAIEDSGAGDITFTFQTGTSSPKNATKVITLNRTADGVWACKSTQDPMFTPKGCDN
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
蛋白标签：

N-terminal 10xHis-tagged and C-terminal Myc-tagged
产品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
缓冲液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
复溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
储存条件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保质期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
货期：

3-7 business days
注意事项：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

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功能：

Major component of the type IV pilus (T4P) that plays a role in surface and host cell adhesion, colonization, biofilm maturation, virulence, and twitching, a form of surface-associated motility facilitated by cycles of extension, adhesion, and retraction of T4P fibers. In addition, plays a critical role in type II secretion of exoenzymes by interacting with specific components such as XcpT or XcpU. Modulates host calcium signaling through interaction with host calcium-modulating cyclophilin ligand.
基因功能参考文献：
1. Glycosylation of type 4 fimbrial protein (PilA) protect Pseudomonas aeruginosa from certain pilus-specific phages. Alterations to PilA sequence can block phage infection, but glycosylation is less likely to create disadvantageous phenotypes. Through construction of chimeric phages, specific phage tail proteins allow infection of P. aeruginosa strains with glycosylated pili. PMID: 29133883
2. PilS's phosphatase motif was required for the autoregulation of pilA transcription, suggesting that under conditions where PilA is abundant, the PilA-PilS interaction promotes PilR dephosphorylation and thus down-regulation of further pilA transcription. PMID: 27162347
3. Data show that the type IV pilus (Tfp)-deficient mutant DeltapilA is severely attenuated in an acute pneumonia model of infection in the lungs of wild-type mice, but is virulent in the lungs of surfactant protein-A SP-A(-/-) mice. PMID: 24080545
4. Found that the calcium-modulating cyclophilin ligand (CAMLG), which is involved in Ca(2+) signaling, was the major host cell PilA binding protein. PMID: 23266901
5. PilA, the major pilin subunit, interacts with PilNOPQ. The results allow us to propose a model whereby PilA makes extensive contacts with the transenvelope complex, possibly to increase local concentrations of PilA monomers for polymerization. PMID: 23457250
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亚细胞定位：

Fimbrium. Cell inner membrane; Single-pass membrane protein. Host endoplasmic reticulum membrane.
蛋白家族：

N-Me-Phe pilin family
数据库链接：

KEGG: pae:PA4525

STRING: 208964.PA4525

Recombinant Pseudomonas aeruginosa Type IV major pilin protein PilA (pilA), partial

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