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  4. Recombinant Probable glucosyl transferase GtfB (gtfB)

Recombinant Probable glucosyl transferase GtfB (gtfB)

  • 中文名称：
    Recombinant Probable glucosyl transferase GtfB(gtfB)
  • 货号：
    CSB-YP741780SML
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Probable glucosyl transferase GtfB(gtfB)
  • 货号：
    CSB-EP741780SML
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Probable glucosyl transferase GtfB(gtfB)
  • 货号：
    CSB-EP741780SML-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Probable glucosyl transferase GtfB(gtfB)
  • 货号：
    CSB-BP741780SML
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Probable glucosyl transferase GtfB(gtfB)
  • 货号：
    CSB-MP741780SML
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    gtf2
  • Uniprot No.：
    Q79T00
  • 别名：
    gtfB; UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase stabilizing protein GtfB; Glycosyltransferase stabilizing protein GtfB; orf4
  • 种属：
    Streptococcus gordonii
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-450
  • 氨基酸序列
    MIQLFDYYNQ ETQDLHDSLL AAGYACPTIV IEANGFLPDD MISPYTYFLG DEEGVDHPLF FNQVPVPPFW EITGDHQVAR VSDMGEERAR IHYASQARGR LVKQVDWLDK KGQLRLSERY NKQGRCFAKT AYKSGQEAFN TTYYSTDGQE RIVENHVTGD IILTLDQEPL RIFKSRVDFI RFFLERLDLD LDHILFNSLA YSFLVSHSLT GRAGQDILFW QEPLYDELPG NMQLILDNSQ LRTQTIVIPD LATYEKAMSL AAADQQQKFL HLGYHYDFKR DNYLRKDALI LTHSDQIEGL DTLVQSLPQL VFRIAALTEM SPKLLSMLSY KNVVLYQNAS LKQIEQLYLE SDIYLDINHG GQVLQAVRKA FENNLLILGF EQTLHDRHYI AQQHIFDSSQ PAQLASILEE ALCGVEQMRS ALQAQGRHAN DVPVSLYQET LQSLLGGQHG
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Required for polymorphic O-glycosylation of GspB, a serine-rich repeat cell wall protein encoded upstream in the same operon. A substrate-binding protein that is part of the accessory SecA2/SecY2 system specifically required to export GspB. The GtfA-GtfB complex adds GlcNAc from UDP-GlcNAc to GspB, attaching the first sugar residue. Upon coexpression in E.coli with GtfA glycosylates GspB constructs. Binds the GspB protein substrate; alone this subunit only recognizes partially glycosylated GspB, but is constrained by GtfA to also recognize unglycosylated protein. The enzyme probably modifies its tertiary conformation by opening and closing its intersubunit interfaces to accomodate the increasingly glycosylated substrate (Probable).
  • 亚细胞定位：
    Cell membrane; Peripheral membrane protein.
  • 蛋白家族：
    Gtf2 family

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