Your Good Partner in Biology Research

中文简体

CSF2｜ CTLA4｜ DLL3｜ EGFR｜ IL4｜ IL4R｜ LAG3｜ MSTN｜ NECTIN4｜ NT5E｜ SEZ6｜ TM4SF1｜ TNFSF13｜ TPO

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Pongo abelii DBIRD complex subunit KIAA1967 homolog, partial

Recombinant Pongo abelii DBIRD complex subunit KIAA1967 homolog, partial

  • 中文名称：
    Recombinant Pongo abelii DBIRD complex subunit KIAA1967 homolog ,partial
  • 货号：
    CSB-YP728974PYX
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Pongo abelii DBIRD complex subunit KIAA1967 homolog ,partial
  • 货号：
    CSB-EP728974PYX
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Pongo abelii DBIRD complex subunit KIAA1967 homolog ,partial
  • 货号：
    CSB-EP728974PYX-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Pongo abelii DBIRD complex subunit KIAA1967 homolog ,partial
  • 货号：
    CSB-BP728974PYX
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Pongo abelii DBIRD complex subunit KIAA1967 homolog ,partial
  • 货号：
    CSB-MP728974PYX
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    CCAR2
  • Uniprot No.：
    Q5R8S0
  • 别名：
    CCAR2Cell cycle and apoptosis regulator protein 2; Cell division cycle and apoptosis regulator protein 2
  • 种属：
    Pongo abelii (Sumatran orangutan)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：
    苏门达腊猩猩DBIRD复合物亚基KIAA1967同源物，也称作ZC3H13，是DBIRD复合物的一个亚基，参与基因转录的调控。如果要重组该蛋白的部分，可以按照以下步骤进行：
    克隆KIAA1967基因片段：可以使用PCR技术从苏门达腊猩猩的基因组DNA中扩增KIAA1967基因片段的序列，包括所需的编码部分以及一些启动子和标签序列。
    构建表达载体：将KIAA1967基因片段插入到表达载体中，可以选择一些经过验证的表达载体，如pET系列载体，这些载体中包含了一些促进蛋白表达的元件，如启动子、增强子、终止子等。
    选择表达宿主细胞：可以选择一些常用的表达宿主细胞，如哺乳动物细胞，例如CHO细胞、HEK293细胞等，并在细胞中转染含有KIAA1967基因片段的表达载体。这些宿主细胞中还需要添加一些辅助蛋白质，如转染脂质、抗生素等，以帮助蛋白质的正确折叠和纯化。
    蛋白表达和纯化：在表达宿主细胞中诱导KIAA1967蛋白的表达，并通过细胞破碎和离心等方法提取蛋白质片段。然后使用亲和层析、离子交换层析、凝胶过滤层析等方法对蛋白进行纯化。
    蛋白质的鉴定和活性检测：使用SDS-PAGE、Western blot等方法对纯化后的KIAA1967蛋白进行鉴定和定量。此外，还可以使用细胞实验等方法对KIAA1967蛋白的生物活性进行检测，如检测其在DBIRD复合物中的作用，以及其对基因转录的调控等。
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Core component of the DBIRD complex, a multiprotein complex that acts at the interface between core mRNP particles and RNA polymerase II (RNAPII) and integrates transcript elongation with the regulation of alternative splicing: the DBIRD complex affects local transcript elongation rates and alternative splicing of a large set of exons embedded in (A + T)-rich DNA regions. Inhibits SIRT1 deacetylase activity leading to increasing levels of p53/TP53 acetylation and p53-mediated apoptosis. Inhibits SUV39H1 methyltransferase activity. Mediates ligand-dependent transcriptional activation by nuclear hormone receptors. Plays a critical role in maintaining genomic stability and cellular integrity following UV-induced genotoxic stress. Regulates the circadian expression of the core clock components NR1D1 and ARNTL/BMAL1. Enhances the transcriptional repressor activity of NR1D1 through stabilization of NR1D1 protein levels by preventing its ubiquitination and subsequent degradation. Represses the ligand-dependent transcriptional activation function of ESR2. Acts as a regulator of PCK1 expression and gluconeogenesis by a mechanism that involves, at least in part, both NR1D1 and SIRT1. Negatively regulates the deacetylase activity of HDAC3 and can alter its subcellular localization. Positively regulates the beta-catenin pathway (canonical Wnt signaling pathway) and is required for MCC-mediated repression of the beta-catenin pathway. Represses ligand-dependent transcriptional activation function of NR1H2 and NR1H3 and inhibits the interaction of SIRT1 with NR1H3. Plays an important role in tumor suppression through p53/TP53 regulation; stabilizes p53/TP53 by affecting its interaction with ubiquitin ligase MDM2. Represses the transcriptional activator activity of BRCA1. Inhibits SIRT1 in a CHEK2 and PSEM3-dependent manner and inhibits the activity of CHEK2 in vitro.
  • 亚细胞定位：
    Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle.
  • 数据库链接：

    STRING: 9601.ENSPPYP00000020648

    UniGene: Pab.2457

Most popular with customers

产品中心
特别关注


X
九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |