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  4. Recombinant Pilin (SPy_0128, M5005_Spy0109)

Recombinant Pilin (SPy_0128, M5005_Spy0109)

  • 中文名称：
    Recombinant Pilin(SPy_0128, M5005_Spy0109)
  • 货号：
    CSB-YP858247SMT
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Pilin(SPy_0128, M5005_Spy0109)
  • 货号：
    CSB-EP858247SMT
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Pilin(SPy_0128, M5005_Spy0109)
  • 货号：
    CSB-EP858247SMT-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Pilin(SPy_0128, M5005_Spy0109)
  • 货号：
    CSB-BP858247SMT
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Pilin(SPy_0128, M5005_Spy0109)
  • 货号：
    CSB-MP858247SMT
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    SPy_0128
  • Uniprot No.：
    Q9A1S2
  • 别名：
    SPy_0128; M5005_Spy0109; Pilin; Lancefield T antigen; Pilus backbone structural protein
  • 种属：
    Streptococcus pyogenes serotype M1
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    24-311
  • 氨基酸序列
    ETVVNGA KLTVTKNLDL VNSNALIPNT DFTFKIEPDT TVNEDGNKFK GVALNTPMTK VTYTNSDKGG SNTKTAEFDF SEVTFEKPGV YYYKVTEEKI DKVPGVSYDT TSYTVQVHVL WNEEQQKPVA TYIVGYKEGS KVPIQFKNSL DSTTLTVKKK VSGTGGDRSK DFNFGLTLKA NQYYKASEKV MIEKTTKGGQ APVQTEASID QLYHFTLKDG ESIKVTNLPV GVDYVVTEDD YKSEKYTTNV EVSPQDGAVK NIAGNSTEQE TSTDKDMTIT FTNKKDFEVP T
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Major component of the pilus. A stack of the pilin subunits, joined by intermolecular isopeptide bonds, forms the pilus. The pilus is required for bacterial adhesion to host cells, for bacterial aggregation, and for biofilm formation.
  • 基因功能参考文献：
    1. an intact Spy0128 is inextensible at any force, peptide-modified Spy0128 pilins readily unfold at very low forces, marking the abrogation of the intramolecular isopeptide bond as well as the absence of a stable pilin fold. PMID: 30150415
    2. crystal structure of the major pilin subunit at 2.2 Angstrom resolution was determined; each subunit contains two lysine-asparagine isopeptide bonds generated by an intramolecular reaction PMID: 18063798
    3. The crystal structure of the major pilin of S. pyogenes, Spy0128, revealed that each domain of the two domain protein contained an intramolecular isopeptide bond cross-link joining a Lys side chain to an Asn side chain. PMID: 19497855
  • 亚细胞定位：
    Secreted, cell wall; Peptidoglycan-anchor. Fimbrium. Note=Attached to the cell wall by a peptidoglycan anchor.
  • 蛋白家族：
    Streptococcus pilin family
  • 数据库链接：

    KEGG: spy:SPy_0128

    STRING: 160490.SPy_0128

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