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  4. Recombinant Oryza sativa subsp. japonica DEAD-box ATP-dependent RNA helicase 2 (Os01g0639100, LOC_Os01g45190)

Recombinant Oryza sativa subsp. japonica DEAD-box ATP-dependent RNA helicase 2 (Os01g0639100, LOC_Os01g45190)

  • 中文名称：
    扁桃体亚属日本产植物EIF4A3A重组蛋白
  • 货号：
    CSB-YP736191OFG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    扁桃体亚属日本产植物EIF4A3A重组蛋白
  • 货号：
    CSB-EP736191OFG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    扁桃体亚属日本产植物EIF4A3A重组蛋白
  • 货号：
    CSB-EP736191OFG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    扁桃体亚属日本产植物EIF4A3A重组蛋白
  • 货号：
    CSB-BP736191OFG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    扁桃体亚属日本产植物EIF4A3A重组蛋白
  • 货号：
    CSB-MP736191OFG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    EIF4A3A
  • Uniprot No.：
    Q5VNM3
  • 别名：
    EIF4A3A; EIF4A3; RH2; Os01g0639100; LOC_Os01g45190; OsJ_02769; P0004A09.21; P0696E01.42Eukaryotic initiation factor 4A-III homolog A; OseIF4AIIIa; eIF-4A-III; eIF4A-III; EC 3.6.4.13; DEAD-box ATP-dependent RNA helicase 2; OsRH2
  • 种属：
    Oryza sativa subsp. japonica (Rice)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-404
  • 氨基酸序列
    MAAATTSRRG PGAMDDENLT FETSPGVEVI SSFDQMGIRD DLLRGIYAYG FEKPSAIQQR AVLPIISGRD VIAQAQSGTG KTSMISLSVC QIVDTAVREV QALILSPTRE LAAQTERVML AIGDFINIQV HACIGGKSIG EDIRKLEHGV HVVSGTPGRV CDMIKRRTLR TRAIKLLILD EADEMLGRGF KDQIYDVYRY LPPELQVCLI SATLPHEILE MTSKFMTDPV RILVKRDELT LEGIKQFFVA VEKEEWKFDT LCDLYDTLTI TQAVIFCNTK RKVDWLTERM RSNNFTVSAM HGDMPQKERD AIMGEFRSGA TRVLITTDVW ARGLDVQQVS LVINYDLPNN RELYIHRIGR SGRFGRKGVA INFVKKEDIR ILRDIEQYYS TQIDEMPMNV ADLI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    ATP-dependent RNA helicase. Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Its RNA-dependent ATPase and RNA-helicase activities are induced by MLN51/CASC3, but abolished in presence of the MAGO-Y14 heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The inhibition of ATPase activity by the MAGO-Y14 heterodimer increases the RNA-binding affinity of the EJC. EJC core proteins play essential roles in rice development, growth and reproduction. Regulates the splicing of UDT1 (UNDEVELOPED TAPETUM 1) pre-mRNA transcript. UDT1 is a key regulator in stamen development.
  • 基因功能参考文献：
    1. Data show that the gene expression patterns OF DEAD box RNA helicases OsRH2 and OsRH34 were similar in various tissues, but the level of OsRH2 mRNA was about 58-fold higher than that of OsRH34 mRNA in seedlings. PMID: 27071313
  • 亚细胞定位：
    Nucleus. Cytoplasm.
  • 蛋白家族：
    DEAD box helicase family, DDX48/FAL1 subfamily
  • 组织特异性：
    Expressed in roots, leaves, flowers and seeds.
  • 数据库链接：

    KEGG: osa:4326580

    STRING: 39947.LOC_Os01g45190.1

    UniGene: Os.39050

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