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  4. Recombinant Oryza sativa subsp. japonica Jasmonoyl--L-amino acid synthetase GH3.5 (GH3.5)

Recombinant Oryza sativa subsp. japonica Jasmonoyl--L-amino acid synthetase GH3.5 (GH3.5)

  • 中文名称：
    Recombinant Oryza sativa subsp. japonica Jasmonoyl--L-amino acid synthetase GH3.5 (GH3.5)
  • 货号：
    CSB-EP743555OFG
  • 规格：
    ￥2328
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 生物活性：
    Not Test
  • 基因名：
    GH3.5
  • Uniprot No.：
    Q6I581
  • 别名：
    Auxin-responsive GH3-like protein 5;OsGH3-5;Indole-3-acetic acid-amido synthetase GH3.5;Jasmonate-amino acid synthetase JAR1;Jasmonic acid-amido synthetase JAR1;Protein JASMONATE RESISTANT 1;OsJAR1
  • 种属：
    Oryza sativa subsp. japonica (Rice)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    72.0 kDa
  • 表达区域：
    1-581aa
  • 氨基酸序列
    MTICSCEETINEFEMLTRDAARVQKDTLKKILEINASAEYLQNFGLGGRTDAESYKSCIPLCVHNDIEPYIQRIVDGDTSPVVTGEPITNLSLSSGTTHGKPKFIPFNDELLETTLQIYRTSYAFRNREYPIGQGKALQFVYGSKQVITKGGILATTATTNLYRRQRYKEGMKDIQSQCCSPDEVIFGPDFHQSLYCHLLCGLIYSEEVHSVFSTFAHSLVHAFQTFEEVWEDLCTDIRDGVLSKKVTAPSIREAVSKILKPNPELADSIYKKCIGLSNWYGVIPALWPNAKYVYGIMTGSMEPYLKKLRHYAGNLPLISADYGASEGWVGSNIDPTVPPEQVTYAVLPQVGYFEFIPLEKPIGEETENSASIHYIESDPVGLTEVEVGKIYEVVITNFAGLYRYRLGDVVKIARFHNSTPELQFICRRSLVLSINIDKNTEKDLQLAVEEASKFLEGEKLEVMDFTSFVERSSDPGRYVIFWELSGDASDEVLSSCANALDLAFIDAGYTGSRKIKTIGPLELRILRKGTFKEILDHFLSLGGAVSQFKTPRFVNPSNSKVLQILSRNVTQSYFSTAYGF
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    C-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

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GH3.5 Proteins

靶点详情

  • 功能：
    Catalyzes the synthesis of jasmonate-amino acid conjugates by adenylation. Catalyzes the conjugation of jasmonate (JA) to Ile when expressed in a heterologous system (E.Coli). Catalyzes in vitro the conjugation of jasmonate (JA) to Ile, Phe, Cys, Leu, Met, Ala, Val and Trp. Involved in the production of JA-Ile in response to infection by the rice blast fungus Magnaporthe oryzae. Required for the accumulation of the flavonoid phytoalexin sakuranetin in response to infection by the rice blast fungus. Involved in herbivory-induced JA-Ile accumulation. Involved in the production of JA-Ile in response to wounding. Required for modulation of light and JA signaling in photomorphogenesis. Required for normal seed development. Required for optimal flower opening and closing and anther dehiscence. May catalyze the synthesis of indole-3-acetic acid (IAA)-amino acid conjugates, providing a mechanism for the plant to cope with the presence of excess auxin.
  • 蛋白家族：
    IAA-amido conjugating enzyme family
  • 组织特异性：
    Expressed in green shoots, roots and flowers.
  • 数据库链接：

    KEGG: osa:4339756

    STRING: 39947.LOC_Os05g50890.1

    UniGene: Os.81757

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