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  4. Recombinant Mouse Unconventional myosin-Va (Myo5a), partial

Recombinant Mouse Unconventional myosin-Va (Myo5a), partial

  • 中文名称：
    小鼠Myo5a重组蛋白
  • 货号：
    CSB-YP857430MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Myo5a重组蛋白
  • 货号：
    CSB-EP857430MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Myo5a重组蛋白
  • 货号：
    CSB-EP857430MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Myo5a重组蛋白
  • 货号：
    CSB-BP857430MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Myo5a重组蛋白
  • 货号：
    CSB-MP857430MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Myo5a
  • Uniprot No.：
    Q99104
  • 别名：
    Myo5a; Dilute; Unconventional myosin-Va; Dilute myosin heavy chain; non-muscle
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Processive actin-based motor that can move in large steps approximating the 36-nm pseudo-repeat of the actin filament. Involved in melanosome transport. Also mediates the transport of vesicles to the plasma membrane. May also be required for some polarization process involved in dendrite formation.
  • 基因功能参考文献：
    1. When actin and microtubules were present simultaneously, melanophilin's phosphorylation state enforced track selection of the Rab27a/melanophilin/myosin Va transport complex. PMID: 28559319
    2. In the motor domain-IQ1/Ca(2+)-CaM structure, the N-lobe and the C-lobe of Ca(2+)-CaM adopt an open conformation and grip the C-terminal and the N-terminal portions of the IQ1, respectively. PMID: 27647889
    3. The authors determined crystal structures of MyoVa-globular tail domain bound either to the Spir-2 motif or to Rab11 and show that a Spir-2:MyoVa:Rab11 complex can form. PMID: 27623148
    4. function of two coupled myosin va motors on actin filaments and bundles PMID: 27851945
    5. MyoVa-Ca(2+) channel interactions are required for proper long-range axon growth in developing spinal cord in vivo. PMID: 27134178
    6. Myo5c-globular tail domain and Myo5a-globular tail domain are not interchangeable in terms of inhibiting the motor function. PMID: 26912658
    7. This study showed that spontaneous Myo5a mutations causing cerebellar pathology are impaired in motor functions during the neonatal period. PMID: 25907855
    8. These results visualize many of the critical unknown aspects of the stepping mechanism of myosin 5 including head-head coordination, the origin of lever-arm motion and the spatiotemporal dynamics of the translocating head during individual steps. PMID: 25748137
    9. deletion mutation results in Griscelli syndrome type 3-like phenotype PMID: 25283056
    10. Motor coupling through lipid membranes enhances transport velocities for ensembles of myosin Va. PMID: 25201964
    11. Microtubules retard a centrifugal transport process that is dependent on myosin-Va and a population of dynamic F-actin. Functional analysis of mutant proteins indicates that myosin-Va works as a transporter dispersing melanosomes along actin tracks. PMID: 25065759
    12. Normal physiological processes of relaxation of gastric and cavernosal smooth muscles that facilitate food accommodation and penile erection, respectively, may be disrupted under conditions of myosin Va deficiency. PMID: 24516539
    13. Data demonstrate that myosin-Va is a component of a heterogeneous population of ribonucleoprotein particles of different sizes and composition, both in brain and peripheral nervous system PMID: 24272908
    14. Myosin Va associates with FMRP and is required for its correct intracellular localisation. PMID: 24175909
    15. MYO5A plays a role in the surface trafficking of Kv1.5 and connexin-43 but not KCNH2. It participates in anterograde trafficking PMID: 24508725
    16. Data show at the single-molecule level that melanophilin (Mlph) increases the number of processively moving myoVa motors by 17-fold. PMID: 23979131
    17. Introduction of a myosin 2-specific repulsive interaction into myosin 5a via the I67K mutation perturbs the strong-binding interaction of myosin 5a with actin. PMID: 23650521
    18. This study demonistrated that Myosin Va mutant mouse with disruptions in glutamate synaptic development and mature plasticity in visual cortex in mice. PMID: 23658184
    19. myosin Va has no non-redundant role in the secretion of dense and alpha-granules or other functional responses in platelets. PMID: 23349704
    20. Data suggest that Rab27a and Rab27b regulate distinct steps in the bone marrow-derived mast cells (BMMC) degranulation pathway, with Rab27a/Mlph/MyoVa regulating cortical actin stability upstream of Rab27a/b/Munc13-4-dependent granule exocytosis. PMID: 23281710
    21. These data show a correlation between the subsynaptic accumulation of myosin Va and PKA type I. PMID: 22815846
    22. Multiple Rab proteins regulate the trafficking of GLUT4, with Rab10 and myosin-Va mediating the final steps of insulin-stimulated vesicle translocation to the plasma membrane. PMID: 22908308
    23. Calmodulin bound to the first IQ motif is responsible for calcium-dependent regulation of myosin 5a. PMID: 22437832
    24. Without cargo at near physiologic ionic strength (100 mM KCl), full-length myoV adopts a folded (approximately 15 S), enzymatically-inhibited state that unfolds to an extended (approximately 11 S), active conformation at higher salt PMID: 22228305
    25. The stepping dynamics of single quantum-dot-labeled myoV and myoVI motors linked to a common cargo, was studied. PMID: 21808051
    26. Myo Va interactions with intermediate filament proteins may serve similar roles in organizing organelle topography in different cell types. PMID: 21359212
    27. Myo5a and Rab3A are direct binding partners and interact on synaptic vesicles and the Myo5a/Rab3A complex is involved in transport of neuronal vesicles PMID: 21349835
    28. myosin Va function is critical for subsequent processes in localization of smooth endoplasmic reticulum and IP(3) receptors in Purkinje cell spines, long-term depression, and motor learning. PMID: 21508232
    29. Data show that myosin-Va acts as a point-to-point organelle transporter to pull ER as cargo into Purkinje neuron spines. PMID: 21151132
    30. the class-specific switch-2 structure including a tyrosine (Y439) in myosin-5 enables rapid processive translocation along actin filaments by facilitating Mg(2+)-dependent ADP release PMID: 20631329
    31. Using an optical trap-based assay of single myosin-Va motor heads study showed that by applying load to the head shortly after binding to actin, the head fluctuated between an actin-bound pre- and a post-power stroke conformation. PMID: 20418880
    32. Data show that myosin 5a walking along actin filaments by using electron microscopy. PMID: 20133809
    33. myosin Va mediates correct positioning of PKA RIalpha in a postsynaptic microdomain, presumably by tethering PKA RIalpha to the actin cytoskeleton PMID: 20133847
    34. These results show that the actin D-loop strongly modulates the force generation by myosin II and the processivity of myosin V. PMID: 19840951
    35. The leaden gene product is required with Rab27a to recruit myosin Va to melanosomes in melanocytes PMID: 11886590
    36. myosin Va binds to melanophilin and Rab27a PMID: 11887186
    37. myosin-V has a broader function in melanocytes by acting on vesicular targeting or intracellular protein trafficking. PMID: 11921164
    38. Melanophilin, the product of the leaden locus, is required for targeting of myosin-Va to melanosomes PMID: 11929602
    39. Identification of mRNA/protein (mRNP) and their association with rough endoplasmic reticulum equipped with a kinesin motor. PMID: 12147688
    40. These findings suggested that MyoVa is able to bind to more than one type of cargo, with the selectivities determined by alternative spliced sequences. PMID: 12211107
    41. Slac2c interacts with this protein, actin and myosin VIIa and rab27 PMID: 12221080
    42. myosin Va advances along axons at slow transport rates overlapping with those of neurofilament proteins and actin PMID: 12403814
    43. Regulatory implications of a novel mode of interaction of calmodulin with a double IQ-motif target sequence from murine dilute myosin V PMID: 12441389
    44. Electron microscopic analysis of mouse myosin V conformation PMID: 12499355
    45. myosin V has a folded conformation that is coupled to inhibition of its enzymatic activity in the absence of cargo and Ca(2+). PMID: 14634000
    46. The acroplaxome provides a focal point for myosin-Va/ Rab27a/b-driven proacrosomal vesicles to accumulate, coalesce, and form an acrosome in wild-type spermatids and a pseudoacrosome in Hrb mutant spermatids. PMID: 14724135
    47. Myosin Va is required for normal photoreceptor signalling. PMID: 15316067
    48. MYO5A is nonessential for melanosome transfer, although pigment incorporation into the hair in MYO5A-deficient mice is uneven PMID: 15550542
    49. The myosin Va binding domain (MBD) is sufficient for the activation of myosin Va. Among MBDs, the interaction of MBD-2 with exon-F of myosin Va is critical for the binding of myosin Va and melanophilin. PMID: 15760894
    50. Results imply that a positively charged loop 2 and a high affinity for actin are important to maintain myosin V processivity near physiologic ionic strength. PMID: 16377634

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  • 蛋白家族：
    TRAFAC class myosin-kinesin ATPase superfamily, Myosin family
  • 组织特异性：
    Detected in melanocytes.
  • 数据库链接：

    KEGG: mmu:17918

    STRING: 10090.ENSMUSP00000116028

    UniGene: Mm.3645

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