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  4. Recombinant Mouse Tyrosine-protein kinase JAK2 (Jak2), partial

Recombinant Mouse Tyrosine-protein kinase JAK2 (Jak2), partial

  • 中文名称：
    小鼠Jak2重组蛋白
  • 货号：
    CSB-YP726782MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Jak2重组蛋白
  • 货号：
    CSB-EP726782MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Jak2重组蛋白
  • 货号：
    CSB-EP726782MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Jak2重组蛋白
  • 货号：
    CSB-BP726782MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Jak2重组蛋白
  • 货号：
    CSB-MP726782MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Jak2
  • Uniprot No.：
    Q62120
  • 别名：
    Jak2; Tyrosine-protein kinase JAK2; EC 2.7.10.2; Janus kinase 2; JAK-2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Non-receptor tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. Mediates essential signaling events in both innate and adaptive immunity. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors such as growth hormone (GHR), prolactin (PRLR), leptin (LEPR), erythropoietin (EPOR), thrombopoietin (THPO); or type II receptors including IFN-alpha, IFN-beta, IFN-gamma and multiple interleukins. Following ligand-binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its cytoplasmic domain. Then, STAT5 (STAT5A or STAT5B) is recruited, phosphorylated and activated by JAK2. Once activated, dimerized STAT5 translocates into the nucleus and promotes the transcription of several essential genes involved in the modulation of erythropoiesis. Part of a signaling cascade that is activated by increased cellular retinol and that leads to the activation of STAT5 (STAT5A or STAT5B). In addition, JAK2 mediates angiotensin-2-induced ARHGEF1 phosphorylation. Plays a role in cell cycle by phosphorylating CDKN1B. Cooperates with TEC through reciprocal phosphorylation to mediate cytokine-driven activation of FOS transcription. In the nucleus, plays a key role in chromatin by specifically mediating phosphorylation of 'Tyr-41' of histone H3 (H3Y41ph), a specific tag that promotes exclusion of CBX5 (HP1 alpha) from chromatin.
  • 基因功能参考文献：
    1. SOCS2 negatively regulates the development of natural killer cells by inhibiting JAK2 activity via direct interaction. PMID: 28383049
    2. HSP27 is a partner of JAK2-STAT5 and a potential therapeutic target in myelofibrosis. PMID: 29650953
    3. Janus kinase 2 protein (JAK2) and beta-catenin were found to interact with cadherin-22 (Cdh22) and involved in CDH22 signaling in female germ line stem cells (FGSCs). PMID: 29063123
    4. While expression of mutant Jak2 was necessary for leukemia induction, neither its continued expression nor enzymatic activity was required to maintain leukemia survival and rapid proliferation PMID: 29907650
    5. High JAK2 expression is associated with graft-versus-host disease. PMID: 29382747
    6. Results demonstrate that JAK2 deficiency delays tumor formation in GH-transgenic mice, which is linked to insignificant oncogenic STAT3 signaling and reduced reactive oxygen species-induced oxidative damage. PMID: 27713471
    7. Reg3g acts as an immunosuppressive promoter by activating the JAK2/STAT3 signaling pathway in dendritic cells and triggering the generation of an immunosuppressive tumor microenvironment. PMID: 28880262
    8. High JAK2 expression is associated with skeletal muscle wasting in cancer cachexia. PMID: 28489606
    9. Data (including data from studies in knockout mice) suggest that various tissues utilize Jak2 signaling to regulate insulin, glucose, and lipid metabolism; adipocyte Jak2 appears to regulate hepatic insulin resistance in mechanism independent of adiposity (obesity), liver lipid content (non-alcoholic fatty liver disease), and hepatic insulin signaling. PMID: 29203511
    10. Advanced glycation end products induce apoptosis and inflammation in mouse podocytes through CXCL9-mediated JAK2/STAT3 pathway activation. PMID: 28849106
    11. Cilostazol protects MIR injury by activating a PPARgamma/JAK2/STAT3 pathway. PMID: 28810537
    12. These results suggest that JAK2 regulated the microfilaments aggregation during the mouse oocyte maturation. PMID: 28615348
    13. alpha7nAChR stimulation reduced neuroinflammation via activation of the JAK2-STAT3 pathway, thereby ameliorating the short- and long-term sequelae after intracerebral hemorrhage . PMID: 28529954
    14. After Dox treatment, the protein expression of p-Jak2 and p-Stat3 levels was significantly increased in MT(+/+) cardiomyocytes, suggesting that the JAK2/STAT3 pathway was partially involved in MT protection against Dox-induced cardiotoxicity PMID: 26526549
    15. CIS interacted with phosphorylated EpoR at Y401, which was critical for the activation of STAT5 and ERK. PMID: 28038963
    16. Cell volume homeostasis requiring Na+/H+ exchange signaled by JAK2 first becomes prominent during mouse embryonic development at the late one-cell stage. PMID: 28339658
    17. a novel signaling axis that regulates JAK2 in normal and malignant HSPCs and suggest new therapeutic strategies for treating CBL(mut) myeloid malignancies. PMID: 28611190
    18. In summary, JAK2 functions in a homeostatic capacity in podocytes by facilitating autophagy. It does this by regulating the expression of the transcription factor TFEB that is necessary for normal autophagic-lysosomal function PMID: 28424277
    19. these findings demonstrate that expression of Hmga2 cooperates with Jak2(V617F) in the pathogenesis of Mmyelofibrosis. PMID: 28637665
    20. Study shows that Jak2 with V617F mutation induces murine model for myeloproliferative neoplasm. PMID: 28668884
    21. results suggest that loss of Ezh2 cooperates with Jak2V617F in the development of myelofibrosis in Jak2V617F PMID: 27081096
    22. JAK2-V617F-expressing mice treated with an Ezh2 inhibitor showed higher platelet counts than vehicle controls. Our data support the proposed tumor suppressor function of EZH2 PMID: 27401344
    23. JAK2 has a role in hepatocarcinogenesis through regulating critical inflammatory pathways PMID: 28100771
    24. nascent hematopoietic stem cells are resistant to the JAK2V617F mutation and show no decrease in repopulation or self-renewal and no increase in DNA damage, even in the presence of 2 mutant copies. PMID: 26864339
    25. The results showed that AG490 inhibited (p)-JAK2 and RANKL expression. PMID: 28278513
    26. these results have revealed that phosphorylation of Tyr-343, Tyr-460, and Tyr-464 in EpoR underlies JAK2 V617F mutant-induced tumorigenesis. PMID: 27998978
    27. this study shows that ampelopsin reduces inflammatory response of microglial cells through inhibition of JAK2-STAT3 signaling PMID: 27998743
    28. At the molecular level, administration of liraglutide induced the expression of PDX-1, MafA, p-JAK2 and p-Stat3 in high-fat diet-induced diabetes model to relatively normal levels. PMID: 28290604
    29. this study shows that resokaempferol inhibits the inflammatory response in activated macrophages by blocking the activation of the JAK2 pathway PMID: 27261558
    30. Data show that protein tyrosine phosphatase 1B (PTP1B)-deficiency enhanced JAK2-STAT3 signaling in hypothalamic microglia and attenuated inflammation. PMID: 28094236
    31. Fos-zippered GHR tails and Jak2, both purified from baculovirus-infected insect cells, interacted via box1 with a binding affinity of approximately 40nM. PMID: 26859362
    32. Single agent BMS-911543 Jak2 inhibitor has distinct inhibitory effects on STAT5 signaling in genetically engineered mice with pancreatic ductal carcinomas. PMID: 26575024
    33. JAK2 plays a critical role in brown adipose tissue function and adaptive thermogenesis PMID: 26515423
    34. The critical role of Jak2 in mediating prolactin-induced luminal maturation of mammary epithelial cells. PMID: 26318719
    35. It increases inflammatory markers in microglia, so that targeting it may be a potential strategy for reducing neuroinflammation in Alzheimer's disease. PMID: 26227742
    36. Findings indicate that CD24(+) antigen cells play a role in tumor migration and metastasis and support Janus kinase 2 protein (JAK2) as a therapeutic target in ovarian cancer. PMID: 25969154
    37. JAK2(V617I) resulted in cytokine hyperresponsiveness without constitutive activation in the absence of ligand, whereas JAK2(V617F) resulted in constitutive activation PMID: 26458983
    38. The pro-inflammatory cytokine, interferon-gamma may contribute to diarrhea associated with intestinal inflammation in part through regulation of the epithelial aquaporin-1 water channel via a non-classical JAK/STAT receptor signalling pathway. PMID: 25793528
    39. JAK2 participates in the signalling, regulating the voltage-gated K(+) channel KCNA3. PMID: 25644777
    40. PRDX6 promotes lung tumor development via its mediated and CCL5-associated activation of the JAK2/STAT3 pathway. PMID: 25582888
    41. The data support an important role of LIF-JAK2-STAT3 regulation in the development of colonic adenocarcinoma-related cachexia. PMID: 26092726
    42. Studied the liver-specific role of PTP1B in GH signaling; found under 48-hour fasting GH-resistant conditions, GH stimulation in vivo led to a robust stimulation of the JAK-STAT signalling pathway. PMID: 24948418
    43. HDAC2 might be a downstream effector of Jak2 to mediate cardiac hypertrophic response by pressure overload or Ang-II. PMID: 25380525
    44. Deletion of Stat3 in hematopoietic cells from JAK2-V617F mice did not ameliorate the course of Myeloproliferative neoplasms, but rather enhanced thrombocytosis and shortened the overall survival. PMID: 25595737
    45. these findings demonstrate that inhibiting the JAK2/STAT3 signaling pathway is protective against noise-induced cochlear tissue damage and loss of hearing sensitivity. PMID: 25275304
    46. This work provides insight into the functional consequences of JAK2V617F-TET2 comutation in myeloproliferative neoplasms PMID: 25281607
    47. Mpl expression, but not Tpo, is fundamental in the development of JAK2V617F(+) myeloproliferative neoplasms PMID: 25339357
    48. Our studies demonstrate that MPN can be initiated from a single HSC and illustrate that JAK2-V617F has complex effects on HSC biology. PMID: 25288396
    49. Jak2 plays a critical role in the maintenance and function of adult hematopoietic stem cells. PMID: 24677703
    50. We demonstrated that JAK2V617F-harboring cells cause paracrine DNA damage accumulation through secretion of lipocalin-2. PMID: 25217696

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  • 亚细胞定位：
    Endomembrane system; Peripheral membrane protein. Cytoplasm. Nucleus.
  • 蛋白家族：
    Protein kinase superfamily, Tyr protein kinase family, JAK subfamily
  • 组织特异性：
    Ubiquitously expressed throughout most tissues.
  • 数据库链接：

    KEGG: mmu:16452

    STRING: 10090.ENSMUSP00000025705

    UniGene: Mm.275839

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