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  4. Recombinant Mouse Twinkle protein, mitochondrial (Peo1)

Recombinant Mouse Twinkle protein, mitochondrial (Peo1)

  • 中文名称：
    小鼠Twnk重组蛋白
  • 货号：
    CSB-YP809302MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Twnk重组蛋白
  • 货号：
    CSB-EP809302MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Twnk重组蛋白
  • 货号：
    CSB-EP809302MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Twnk重组蛋白
  • 货号：
    CSB-BP809302MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Twnk重组蛋白
  • 货号：
    CSB-MP809302MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Twnk
  • Uniprot No.：
    Q8CIW5
  • 别名：
    Twnk; Peo1Twinkle protein; mitochondrial; EC 3.6.4.12; Progressive external ophthalmoplegia 1 protein homolog; T7 gp4-like protein with intramitochondrial nucleoid localization; T7-like mitochondrial DNA helicase; Twinkle mtDNA helicase
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    32-685
  • 氨基酸序列
    APGPPRRRY RKEALPALEM PVSPVTTTEI RQYLRAHGIP FQDGHSCLRA PSPFVVSSDI KNEKKDAPTS FCLFIDKTTG HFLCMTSLAE GSWEDLQASV EGRGDGAKEG VLLREGPEAE VREEVLRIWN RAIPLWELPD PEEAQLARVM FGLTKVTDDT LRRFSVRYLR SARSLVFPWF TPGSSGLRGL KLLGAEGQEN GVQYVETTIP RPGVYHNLFG LPLISRRDTE VVVTSRELDS LALSQSTGLP TLSLPRGTVC LPPALLPYLE QFRRIVFWLG DDLRSWEAAK LFARKLNPKR CSLVRPGNQQ PRPLEALNQG LSLPRILRTA LPAWHKSIVS FRQLREEVLG ELSNVEQAAG VRWSRFPDLN RLLKGHRKGE LTVFTGPTGS GKTTFISEYA LDLCTQGVNT LWGSFEISNV RLARVMLTQF AVTRLEEQLD KYEEWADRFE DLPLYFMTFH GQQSIRSVID TMQHAVYVYD VCHVVIDNLQ FMMGHEQLSS DRIAAQDYIV GAFRKFATDN SCHVTLVIHP RKEDDDKELQ TASIFGSAKA SQEADNVLIL QDRKLVTGPG KRYLQVSKNR FDGDVGVFPL EFNKNSLTFS IPPKSKARLK KIKDDNGLVA KKSSSGKKGA AHQNPEICLG QDPSPAQPDT SKSSG
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Mitochondrial helicase involved in mtDNA replication and repair. Might have a role in mtDNA repair. Has DNA strand separation activity needed to form a processive replication fork for leading strand synthesis which is catalyzed by the formation of a replisome complex with POLG and mtSDB. Preferentially unwinds DNA substrates with pre-existing 5'-and 3'- single-stranded tails but is also active on a 5'- flap substrate. Can dissociate the invading strand of immobile or mobile D-loop DNA structures irrespective of the single strand polarity of the third strand. In addition to its DNA strand separation activity, also has DNA strand annealing, DNA strand-exchange and DNA branch migration activities.
  • 基因功能参考文献：
    1. Twinkle overexpression alleviated impaired mitochondrial biogenesis in the border area through maintained mtDNA copy number and thereby prevented cardiac rupture accompanied by the reduction of apoptosis and oxidative stress, and suppression of MMP activity. PMID: 27342873
    2. Twinkle overexpression results in increased mtDNA copy number and facilitates cardioprotection associated with limited mitochondrial oxidative stress. PMID: 25822152
    3. study describes findings in a family with late onset parkinsonism and progressive external ophthalmoplegia, with mutation of G1750A in the exon 1 of PEO1 gene PMID: 24076137
    4. TWINKLE is essential for nascent H-strand synthesis in the D-loop, thus showing that there is no separate DNA helicase responsible for replication of this region. PMID: 23393161
    5. mammalian Twinkle is important for mitochondrial integrity in DA neurons and provide a novel mouse model in which increased mtDNA deletions may lead to DA neuron degeneration and parkinsonism. PMID: 22949510
    6. Essential for mtDNA maintenance, may be a key regulator of mtDNA copy number. PMID: 15509589
    7. generated transgenic mice expressing mouse Twinkle with progressive external ophthalmoplegia patient mutations PMID: 16301523

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  • 亚细胞定位：
    Mitochondrion matrix, mitochondrion nucleoid.
  • 组织特异性：
    Ubiquitous with the highest levels in the liver, heart and kidneys. The skeletal muscle, brain and testis showed lower but detectable expression. Expression is coregulated with MRPL43.
  • 数据库链接：

    KEGG: mmu:226153

    STRING: 10090.ENSMUSP00000026227

    UniGene: Mm.105585

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