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  4. Recombinant Mouse Src substrate cortactin (Cttn)

Recombinant Mouse Src substrate cortactin (Cttn)

  • 中文名称：
    Recombinant Mouse Src substrate cortactin(Cttn)
  • 货号：
    CSB-YP720150MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Mouse Src substrate cortactin(Cttn)
  • 货号：
    CSB-EP720150MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Mouse Src substrate cortactin(Cttn)
  • 货号：
    CSB-EP720150MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Mouse Src substrate cortactin(Cttn)
  • 货号：
    CSB-BP720150MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Mouse Src substrate cortactin(Cttn)
  • 货号：
    CSB-MP720150MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Cttn
  • Uniprot No.：
    Q60598
  • 别名：
    Cttn; Ems1; Src substrate cortactin
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-546
  • 氨基酸序列
    MWKASAGHAV SITQDDGGAD DWETDPDFVN DVSEKEQRWG AKTVQGSGHQ EHINIHKLRE NVFQEHQTLK EKELETGPKA SHGYGGKFGV EQDRMDRSAV GHEYQSKLSK HCSQVDSVRG FGGKFGVQMD RVDQSAVGFE YQGKTEKHAS QKDYSSGFGG KYGVQADRVD KSAVGFDYQG KTEKHESQKD YSKGFGGKYG IDKDKVDKSA VGFEYQGKTE KHESQKDYVK GFGGKFGVQT DRQDKCALGW DHQEKLQLHE SQKDYKTGFG GKFGVQSERQ DSSAVGFDYK ERLAKHESQQ DYAKGFGGKY GVQKDRMDKN ASTFEEVVQV PSAYQKTVPI EAVTSKTSNI RANFENLAKE REQEDRRKAE AERAQRMAKE RQEQEEARRK LEEQARAKKQ TPPASPSPQP IEDRPPSSPI YEDAAPFKAE PSYRGSEPEP EYSIEAAGIP EAGSQQGLTY TSEPVYETTE APGHYQAEDD TYDGYESDLG ITAIALYDYQ AAGDDEISFD PDDIITNIEM IDDGWWRGVC KGRYGLFPAN YVELRQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Contributes to the organization of the actin cytoskeleton and cell shape. Plays a role in the formation of lamellipodia and in cell migration. Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones. Through its interaction with CTTNBP2, involved in the regulation of neuronal spine density. Plays a role in the invasiveness of cancer cells, and the formation of metastases. Plays a role in focal adhesion assembly and turnover. In complex with ABL1 and MYLK regulates cortical actin-based cytoskeletal rearrangement critical to sphingosine 1-phosphate (S1P)-mediated endothelial cell (EC) barrier enhancement. Plays a role in intracellular protein transport and endocytosis, and in modulating the levels of potassium channels present at the cell membrane. Plays a role in receptor-mediated endocytosis via clathrin-coated pits. Required for stabilization of KCNH1 channels at the cell membrane.
  • 基因功能参考文献：
    1. this paper shows that cortactin deficiency causes increased RhoA/ROCK1-dependent actomyosin contractility, intestinal epithelial barrier dysfunction, and disproportionately severe dextran sulfate sodium-induced colitis PMID: 28120846
    2. Secretion of the barrier-stabilising hormone adrenomedullin, which activates Rap1 and counteracts actomyosin contractility, was reduced in plasma from cortactin-deficient mice. Cortactin plays a role in controlling actomyosin contractility with consequences for endothelial barrier integrity. PMID: 27357373
    3. AMPK phosphorylation of cortactin followed by SIRT1 deacetylation modulates the interaction of cortactin and cortical-actin in response to shear stress. Functionally, this AMPK/SIRT1 coregulated cortactin-F-actin dynamics is required for endothelial nitric oxide synthase subcellular translocation/activation and is atheroprotective. PMID: 27758765
    4. Cortactin may have an important role in the development of oral tumors in mice PMID: 27148699
    5. findings reveal that Keap1 regulates cell migration by affecting the subcellular localization and activity of cortactin independently of its role in oxidant stress responses. PMID: 26602019
    6. association of cortactin with Pfn-1 is regulated by c-Abl-mediated cortactin phosphorylation PMID: 24700464
    7. Cell proliferation, migration and invasion were inhibited by genetic knockdown of EMS1. PMID: 24127012
    8. our findings suggest that after GnRHa activation, src activity leads to tyrosine phosphorylation of cortactin, which facilitates its association with Arp3 to engage the actin cytoskeleton. PMID: 24274984
    9. GIT1-cortactin association through GIT1-Spa homology domain is required for cortactin localization to the leading edge and is essential for endothelial cell directional migration and tumor angiogenesis. PMID: 24265417
    10. Data indicate that cortactin as an Src-dependent interacting partner of EB1. PMID: 24144981
    11. Introduction of three 4-R-hydroxyproline residues stabilizes the SH3m-cortactin binding of HPK1 peptide. PMID: 22976002
    12. mXinalpha together with cortactin is able to modulate the p120-catenin activity for shape change and adhesion at the intercalated discs. PMID: 23296090
    13. FAK-mediated focal adhesion remodeling may occur through the formation of a FAK-cortactin signaling complex. PMID: 22952866
    14. ACK1 directly binds and phosphorylates the Arp2/3 regulatory protein cortactin, potentially providing a direct link to Arp2/3-based actin dynamics during EGFR degradation. PMID: 22952966
    15. we demonstrate that cell spreading promotes the association of cortactin and FAK and that tyrosine phosphorylation of cortactin disrupts this interaction, which may explain how it inhibits cell spreading. PMID: 22479425
    16. LPS modulation of cortactin stability is coordinately regulated by stress kinases and the ubiquitin-proteasomal network. PMID: 22514278
    17. MLCK is essential for the translocation and association of cortactin and p47phox. PMID: 22219181
    18. Cortactin is crucial for orchestrating the molecular events leading to proper endothelial barrier function and leukocyte recruitment in vivo. PMID: 21788407
    19. data suggest the possibility that localization of the more tyrosine 466-phosphorylated p85 isoform of cortactin around the heads of elongated spermatids is important for stages VII-VIII processes PMID: 20539040
    20. Regulation of sealing ring formation by L-plastin and cortactin in osteoclasts. PMID: 20650888
    21. Results suggest that MIM promotes ciliogenesis by antagonizing Src-dependent phosphorylation of Cortactin and describe a mechanism linking regulation of the actin cytoskeleton with ciliogenesis and Shh signaling during tissue regeneration. PMID: 20708589
    22. Data suggest that cortactin plays an important role in the mechanics of asymmetric division in oocytes. PMID: 20004659
    23. These results suggest a novel function of phosphorylation-dependent cortactin signaling. PMID: 20041195
    24. Cortactin and connexin 43 (Cx43) were physically linked in seminiferous tubule homogenates and their colocalization in the basal and apical thirds of the seminiferous epithelium was stage-dependent. PMID: 19725064
    25. Cortactin-binding protein 90 (CBP90) expression in the mouse mammary glands during prolactin-induced lobuloalveolar development [CBP90] PMID: 12130822
    26. phosphorylation of cortactin tyrosine residues in the carboxy terminus requires the aminoterminal domain PMID: 12925758
    27. alternative splicing of the F-actin binding domain of cortactin is a new mechanism by which cortactin influences cell migration PMID: 12952985
    28. cortactin is a specific substrate and cooperative effector of Fyn in integrin-mediated signaling processes regulating metastatic potential PMID: 13129922
    29. Erk phosphorylation liberates the SH3 domain of cortactin from intramolecular interactions with proline-rich regions, causing it to synergize with WASP and N-WASP in activating the Arp2/3 complex PMID: 15169891
    30. Cortactin regulates cell movement through activation of N-WASP. PMID: 15585574
    31. A short splicing variant of missing in metastasis binds poorly to cortactin in vitro and is unable to enhance actin polymerization. PMID: 15688017
    32. Our findings suggest that cortactin plays a role in Listeria internalization, but not in the formation of actin clouds and tails, or in bacteria intracellular motility. PMID: 16489553
    33. Cortactin was found to be an early component of the nascent podosome belt, along with dynamin, supporting a role for cortactin in actin assembly. PMID: 16611741
    34. electron microscopy and 3D reconstruction were carried out on F-actin decorated with the actin-binding repeating domain of cortactin, revealing conspicuous density on F-actin attributable to cortactin that is located on a consensus-binding site PMID: 16697006
    35. The results indicate that the transition from a polygonal to an elongated shape entails the acquisition of a dynamic cortical actin cytoskeleton that involves the tyrosine kinase-dependent phosphorylation of cortactin. PMID: 16733791
    36. Targeting of cortactin to BK channels via a novel, noncanonical SH3 domain binding motif has important implications for the coordination of BK channel function in normal physiology and disease PMID: 17065230
    37. Formation of functional invadopodia requires interaction between cortactin and filamentous actin. Phosphorylation of cortactin by Src is essential for functional invadopodia formation. PMID: 17343955
    38. Results provide novel genetic evidence that a ROS-Fer signaling arm contributes to SFK-mediated cortactin tyrosine phosphorylation in integrin signaling. PMID: 17606629
    39. CD44 is an important regulator of HGF/c-Met-mediated in vitro and in vivo barrier enhancement, a process with essential involvement of Tiam1, Rac1, dynamin 2, and cortactin. PMID: 17702746
    40. These results are in disagreement with the proposed model of the cortactin molecule and have important implications for our understanding of cortactin regulation of cytoskeleton dynamics. PMID: 19089942
    41. Cortactin promotes cell migration indirectly, through contributing to activation of selected Rho-GTPases. PMID: 19458196
    42. Depletion of cortactin in mouse embryonic fibroblasts does not profoundly influence actin-dependent cell motility. PMID: 19621352

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  • 亚细胞定位：
    Cytoplasm, cytoskeleton. Cell projection, lamellipodium. Cell projection, ruffle. Cytoplasm, cell cortex. Cell projection. Cell projection, podosome. Cell projection, dendritic spine. Cell projection, dendrite. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction. Membrane, clathrin-coated pit. Cell junction, focal adhesion.
  • 组织特异性：
    Expressed in most tissues, except in B-lymphocytes or plasma cells.
  • 数据库链接：

    KEGG: mmu:13043

    STRING: 10090.ENSMUSP00000099368

    UniGene: Mm.205601

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