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  4. Recombinant Mouse Small RNA 2'-O-methyltransferase (Henmt1)

Recombinant Mouse Small RNA 2'-O-methyltransferase (Henmt1)

  • 中文名称：
    小鼠Henmt1重组蛋白
  • 货号：
    CSB-YP804930MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Henmt1重组蛋白
  • 货号：
    CSB-EP804930MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Henmt1重组蛋白
  • 货号：
    CSB-EP804930MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Henmt1重组蛋白
  • 货号：
    CSB-BP804930MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Henmt1重组蛋白
  • 货号：
    CSB-MP804930MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Henmt1
  • Uniprot No.：
    Q8CAE2
  • 别名：
    Henmt1Small RNA 2'-O-methyltransferase; EC 2.1.1.n8; HEN1 methyltransferase homolog 1; mHEN1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-395
  • 氨基酸序列
    MEMAESIPCN SVVGGNFKEV SPEKVIRFKP PLYKQRYQFV RDLVDRHEPK KVADLGCGDA KLLKLLKIYP CIQLLVGVDI NEEKLHSNGH RLSPYLGEFV KPRDLDLTVT LYHGSVVERD SRLLGFDLIT CIELIEHLDS DDLARFPDVV FGYLSPAMVV ISTPNAEFNP LFPTVTLRDA DHKFEWSRME FQTWALHVAN CYNYRVEFTG VGTPPAGSEH VGYCTQIGVF TKNGGKLSKP SVSQQCDQHV YKPVYTTSYP SLQQEKVLKF VLVGELLIQV DRLRLRYQRM LRDREKDRGP KPGDMDSCPA PHLLLGAVFT EAEKARIESS PKPFCEGEKF YIPLQRLLTY PKLHRLCADE DRVRSLIADS VCLSSDGSAV VVDLHNSWDY RPEEN
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Methyltransferase that adds a 2'-O-methyl group at the 3'-end of piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. This probably protects the 3'-end of piRNAs from uridylation activity and subsequent degradation. Stabilization of piRNAs is essential for gametogenesis.
  • 基因功能参考文献：
    1. DNA damage and a catastrophic deregulation of the haploid germ cell gene expression. Collectively these results define a critical role for HENMT1 and piRNAs in the maintenance of TE repression in adult germ cells and setting the spermatogenic program. PMID: 26496356
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Methyltransferase superfamily, HEN1 family
  • 组织特异性：
    Specifically expressed in testis.
  • 数据库链接：

    KEGG: mmu:66715

    STRING: 10090.ENSMUSP00000054829

    UniGene: Mm.158124

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