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  4. Recombinant Mouse Serine/threonine-protein kinase Nek1 (Nek1), partial

Recombinant Mouse Serine/threonine-protein kinase Nek1 (Nek1), partial

  • 中文名称：
    小鼠Nek1重组蛋白
  • 货号：
    CSB-YP015696MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Nek1重组蛋白
  • 货号：
    CSB-EP015696MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Nek1重组蛋白
  • 货号：
    CSB-EP015696MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Nek1重组蛋白
  • 货号：
    CSB-BP015696MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Nek1重组蛋白
  • 货号：
    CSB-MP015696MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Nek1
  • Uniprot No.：
    P51954
  • 别名：
    Nek1; Serine/threonine-protein kinase Nek1; EC 2.7.11.1; Never in mitosis A-related kinase 1; NimA-related protein kinase 1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Phosphorylates serines and threonines, but also appears to possess tyrosine kinase activity. Involved in DNA damage checkpoint control and for proper DNA damage repair. In response to injury that includes DNA damage, NEK1 phosphorylates VDAC1 to limit mitochondrial cell death. May be implicated in the control of meiosis. Involved in cilium assembly.
  • 基因功能参考文献：
    1. NEK1 phosphorylates PP1gamma, leading to the dephosphorylation of WAPL, which, in turn, results in its retention on chromosome cores to promote loss of cohesion at the end of prophase I in mammals. PMID: 27760328
    2. Nek1 is detectable in all murine tissues but its expression in wild type and kat2J heterozygous kidneys decrease as the kidneys mature. In the embryonic kidney, Nek1 expression is most prominent in cells that will become podocytes and proximal tubules. PMID: 25030234
    3. results demonstrate that Nek1 is important for proper checkpoint control and characterize for the first time a DNA damage response that does not directly involve one of the known upstream mediator kinases, ATM or ATR PMID: 21301226
    4. data suggest that TAZ and Nek1 constitute a negative feedback loop linked through phosphorylation and ubiquitination and that the interaction of Nek1 and TAZ maintain polycystin 2 at the level needed for proper ciliogenesis PMID: 21474562
    5. NEK1 is required for the maintenance of genome stability by acting at multiple junctures, including control of chromosome stability PMID: 21214959
    6. Nek1 may function as a kinase early in the DNA damage response pathway. PMID: 15604234
    7. Murine NIMA-related kinase, Nek1, is larger than previously reported, and that the full-length protein conserves the structural hallmarks of NIMA PMID: 16603261
    8. Nek1 links cell cycle progression and the primary cilium cycle. PMID: 18387364
    9. we propose that Nek1 plays a role in centrosome integrity, affecting both ciliogenesis and centrosome stability PMID: 18533026
    10. These data show that Nek1 is important for efficient DNA damage checkpoint control and for proper DNA damage repair. PMID: 18843199
    11. NEK1 is a candidate to transduce messages from the ciliary-basal body region to the regulation of nuclear gene expression. PMID: 19699716

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  • 亚细胞定位：
    Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
  • 蛋白家族：
    Protein kinase superfamily, NEK Ser/Thr protein kinase family, NIMA subfamily
  • 组织特异性：
    Predominantly in testes (germ cells and Sertoli cells). Lower levels in ovary (oocytes and granulosa cells), thymus and lung.
  • 数据库链接：

    KEGG: mmu:18004

    STRING: 10090.ENSMUSP00000034065

    UniGene: Mm.486881

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