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  4. Recombinant Mouse Semaphorin-3F (Sema3f)

Recombinant Mouse Semaphorin-3F (Sema3f)

  • 中文名称：
    小鼠Sema3f重组蛋白
  • 货号：
    CSB-YP020985MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Sema3f重组蛋白
  • 货号：
    CSB-EP020985MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Sema3f重组蛋白
  • 货号：
    CSB-EP020985MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Sema3f重组蛋白
  • 货号：
    CSB-BP020985MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Sema3f重组蛋白
  • 货号：
    CSB-MP020985MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Sema3f
  • Uniprot No.：
    O88632
  • 别名：
    Sema3f; Semaphorin-3F; Semaphorin IV; Sema IV
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    19-785
  • 氨基酸序列
    AT PIQDQLPATP RVRLSFKELK ATGTAHFFNF LLNTTDYRIL LKDEDHDRMY VGSKDYVLSL DLHDINREPL IIHWAASPQR IEECILSGKD GNGECGNFVR LIQPWNRTHL YVCGTGAYNP MCTYVNRGRR AQALPWTQMQ VVRGRGSRAT DGADRPTPTA PRQDYIFYLE PEKLESGKGK CPYDPKLDTA SALINEELYA GVYIDFMGTD AAIFRTLGKQ TAMRTDQYNS RWLNDPSFIH AELIPDSAER NDDKLYFFFR ERSAEAPQNP AVYARIGRIC LNDDGGHCCL VNKWSTFLKA RLVCSVPGED GIETHFDELQ DVFVQQTQDV RNPVIYAVFT SSGSVFRGSA VCVYSMADIR MVFNGPFAHK EGPNYQWMPF SGKMPYPRPG TCPGGTFTPS MKSTKDYPDE VINFMRTHPL MYQAVYPLQR RPLVVRTGAP YRLTTVAVDQ VDAADGRYEV LFLGTDRGTV QKVIVLPKDD QEVEELMLEE VEVFKEPAPV KTMTISSKRQ QLYVASAVGV THLSLHRCQA YGAACADCCL ARDPYCAWDG QACSRYTASS KRRSRRQDVR HGNPIRQCRG FNSNANKNAV ESVQYGVAGS AAFLECQPRS PQATVKWLFQ RDPSDRRREI RAEDRFLRTE QGLLLRALQL GDRGLYSCTA TENNFKHIVT RVQLHVLGRD AVHAALFPPL AVSVPPPPGT GPPTPPYQEL AQLLAQPEVG LIHQYCQGYW RHVPPRPREA PGALRPPELQ DQKKPRNRRH HPPDT
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

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Sema3f ELISA kits

靶点详情

  • 基因功能参考文献：
    1. data suggest that Sema3f and Nrp2 are dispensable for the exclusion of vessels from the cap mesenchyme during kidney development. PMID: 28929539
    2. Sema3f is suppressed in outer nuclear layer in very low-density lipid-receptor deficient retina. PMID: 28373097
    3. Sema3F functions as an unconventional Sema3 that promotes extraembryonic angiogenesis by inhibiting the Myc-regulated synthesis of Thbs1 in visceral yolk sac epithelial cells. PMID: 28729362
    4. Nrp2 deficiency increased blood vessel permeability and decreased lymphatic vessel drainage during inflammation, highlighting the importance of the NRP2/SEMA3F pathway in the modulation of tissue swelling and resolution of postinflammatory edema. PMID: 27751443
    5. Sema3F plays important roles in the development of neuronal circuitry underlying the regulation of some aspects of anxiety and fear responses. PMID: 26856818
    6. The authors demonstrate that expression of Semaphorin-3F in the outer hair cell region inhibits type I spiral ganglion neuron process extension by activating Neuropilin-2 receptors expressed on spiral ganglion neurons. PMID: 26302206
    7. the embryonically developed wiring deficits in Sema3F mutants persist until adulthood PMID: 25874621
    8. Sema4A promotes Th1 and Th17 differentiation and increases adhesive activation of T cells to endothelial cells, even in the presence of IFN-beta. PMID: 24439904
    9. Sema3F is secreted by early-arriving axons of olfactory sensory neurons and is deposited at the anterodorsal olfactory bulb to repel Nrp2-positive axons that arrive later. PMID: 20550939
    10. These data suggest that Sema3F plays a relatively restricted, if any, role in its receptor expression and postnatal development of the somatosensory cortex, the cerebellum, and the main olfactory system. PMID: 20006658
    11. Sema3F is the principal ligand for Npn-2-mediated axon guidance events in vivo and is a critical determinant of limbic and peripheral nervous system circuitry PMID: 12890759
    12. Sema3F alternatively spliced isoforms share common axonal chemorepulsive properties and binding to neuropilin receptors, and are found to regulate endothelial cell morphology by remodeling lamellipodial protrusions. PMID: 14572462
    13. We describe a related signaling pathway involving secreted class 3 semaphorins, neuropilins, and a plexin receptor, PlexinD1, expressed by endothelial cells PMID: 15239958
    14. Using sema3F-/- mice, we show that Sema3F is critical for vomeronasal sensory neuron axonal fasciculation and for segregation of these sensory afferents from the main olfactory system and lamina-specific targeting of olfactory sensory axons PMID: 15483127
    15. Npn2/Sema3f signaling guides neural crest migration through the somite. PMID: 16319111
    16. Genetic evidence for a Nrp2-Sema3f interaction as a determinant of the wiring of axons of gucy2d+ neurons into the unusual configuration of necklace glomeruli. PMID: 17942483
    17. An important regulator of the ventral tangential cell migration stream, confining the migrating neurons on the telencephalon surface by repelling from the deeper domain. PMID: 18434520
    18. Sema 3D and Sema 3F genes are overlappingly and distinctly expressed in the developing parathyroid and thymus, respectively, and they might play roles in the interactions between these organs and surrounding tissues PMID: 18489001
    19. Semaphorin3F induces significant growth cone collapse of temporal, but not nasal, RGCs expressing high levels of Neuropilin-2. PMID: 18942144
    20. Data demonstrate that methyl-CpG binding protein 2 deficiency disrupts the establishment of neural connections before synaptogenesis by altering semaphorin 3F function. PMID: 19628041
    21. Soluble neuropilin-2Fc did not inhibit repulsion but increased the repellent effect of semaphorin 3F. PMID: 19790074

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Semaphorin family
  • 组织特异性：
    Expressed ubiquitously in adulthood. During embryogenesis, expressed in subregions of the central nervous system and various other tissues like skin, kidney, lung and intestine.
  • 数据库链接：

    KEGG: mmu:20350

    STRING: 10090.ENSMUSP00000079400

    UniGene: Mm.12903

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