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  4. Recombinant Mouse Rho guanine nucleotide exchange factor 2 (Arhgef2), partial

Recombinant Mouse Rho guanine nucleotide exchange factor 2 (Arhgef2), partial

  • 中文名称：
    Recombinant Mouse Rho guanine nucleotide exchange factor 2(Arhgef2) ,partial
  • 货号：
    CSB-YP730666MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Mouse Rho guanine nucleotide exchange factor 2(Arhgef2) ,partial
  • 货号：
    CSB-EP730666MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Mouse Rho guanine nucleotide exchange factor 2(Arhgef2) ,partial
  • 货号：
    CSB-EP730666MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Mouse Rho guanine nucleotide exchange factor 2(Arhgef2) ,partial
  • 货号：
    CSB-BP730666MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Mouse Rho guanine nucleotide exchange factor 2(Arhgef2) ,partial
  • 货号：
    CSB-MP730666MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Arhgef2
  • Uniprot No.：
    Q60875
  • 别名：
    Arhgef2; Kiaa0651; Lbcl1; Lfc; Rho guanine nucleotide exchange factor 2; Guanine nucleotide exchange factor H1; GEF-H1; LBC'S first cousin; Lymphoid blast crisis-like 1; Oncogene LFC; Rhobin
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Activates Rho-GTPases by promoting the exchange of GDP for GTP. May be involved in epithelial barrier permeability, cell motility and polarization, dendritic spine morphology, antigen presentation, leukemic cell differentiation, cell cycle regulation, innate immune response, and cancer. Binds Rac-GTPases, but does not seem to promote nucleotide exchange activity toward Rac-GTPases. May stimulate instead the cortical activity of Rac. Inactive toward CDC42, TC10, or Ras-GTPases. Forms an intracellular sensing system along with NOD1 for the detection of microbial effectors during cell invasion by pathogens. Involved in innate immune signaling transduction pathway promoting cytokine IL6/interleukin-6 and TNF-alpha secretion in macrophage upon stimulation by bacterial peptidoglycans; acts as a signaling intermediate between NOD2 receptor and RIPK2 kinase. Contributes to the tyrosine phosphorylation of RIPK2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Overexpression activates Rho-, but not Rac-GTPases, and increases paracellular permeability. Involved in neuronal progenitor cell division and differentiation. Involved in the migration of precerebellar neurons.
  • 基因功能参考文献：
    1. GEF-H1 was a novel regulator of inflammatory cytokine production and mycobacterial elimination, and may serve as a novel potential target for clinical treatment of tuberculosis. PMID: 28783414
    2. Autophagic regulation of RhoA activity was dependent on GEF-H1 which directly bound to p62 and was degraded by autophagy, resulting in low RhoA activity. In contrast, the loss of autophagy increased GEF-H1 levels and thereby activated RhoA, which caused cells to move by amoeba-like migration. This amoeba-like migration was cancelled by the silencing of GEF-H1. PMID: 27120804
    3. human brain malformation is recapitulated in Arhgef2 mutant mice and identify an aberrant migration of distinct components of the precerebellar system as a pathomechanism underlying the midbrain-hindbrain phenotype. Our results highlight the crucial function of ARHGEF2 in human brain development and identify a mutation in ARHGEF2 as novel cause of a neurodevelopmental disorder. PMID: 28453519
    4. These results identify GEF-H1 as a component of the shear stress response machinery in neutrophils required for a fully competent immune response to bacterial infection. PMID: 27738004
    5. Study describes a new mode of GEF-H1 activation in response to G-protein-coupled receptor (GPCR) ligands that is independent of microtubule depolymerization. PMID: 25209408
    6. Data show that the activation of the microtubule-associated guanine nucleotide exchange factor GEF-H1, encoded by Arhgef2, is essential for sensing of foreign RNA by RIG-I-like receptors. PMID: 24270516
    7. Pak2, but not Pak1, negatively regulates RhoA via phosphorylation of the guanine nucleotide exchange factor GEF-H1 at an inhibitory site, leading to increased GEF-H1 microtubule binding and loss of RhoA stimulation PMID: 23204526
    8. It was shown that the guanine exchange factors GEF-H1 and ECT2 must be downregulated for megakaryote polyploidization. The first (2N-4N) endomitotic cycle requires GEF-H1 downregulation, whereas subsequent cycles (>4N) require ECT2 downregulation. PMID: 22387001
    9. CAPN6 acts as a regulator of Rac1 and cell motility through interaction with GEF-H1. PMID: 21406564
    10. LARG is activated by the Src family tyrosine kinase Fyn, whereas GEF-H1 catalytic activity is enhanced by ERK downstream of a signalling cascade that includes FAK and Ras PMID: 21572419
    11. p114-RhoGEF and Lfc are critically involved in Wnt-3a- and Dvl-induced RhoA activation and neurite retraction in N1E-115 cells. PMID: 20810787
    12. Depletion of GEF-H1 in the murine two-hit model of VILI attenuated vascular leak induced by lung ventilation at high tidal volume and thrombin-derived peptide TRAP6. PMID: 20348280
    13. Enteropathogenic Escherichia coli type III effectors, EspG and its homolog Orf3, trigger the activation of the RhoA-ROCK signaling pathway via GEF-H1 PMID: 15318166
    14. PAK4 mediates morphological changes through regulation of GEF-H1 PMID: 15827085
    15. Microtubule-associated RhoGEF Lfc is required for the formation of the mitotic spindle during prophase/prometaphase. PMID: 15976019
    16. Thus, Lfc and Tctex-1 interact to regulate cortical neurogenesis, potentially by regulating mitotic spindle orientation. PMID: 19448628

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  • 亚细胞定位：
    Cytoplasm, cytoskeleton. Cytoplasm. Cell junction, tight junction. Golgi apparatus. Cytoplasm, cytoskeleton, spindle. Cytoplasmic vesicle.
  • 组织特异性：
    Ubiquitous, with the exception of liver tissue. Levels are high in hemopoietic tissues (thymus, spleen, bone marrow) as well as in kidney and lung. Expressed in the germinal zones of both the neocortex and the cerebellum and in the pontine gray nuclei.
  • 数据库链接：

    KEGG: mmu:16800

    STRING: 10090.ENSMUSP00000029694

    UniGene: Mm.239329

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