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  4. Recombinant Mouse Protein PML (Pml), partial

Recombinant Mouse Protein PML (Pml), partial

In Stock
  • 中文名称：
    小鼠Pml重组蛋白
  • 货号：
    CSB-EP730674MO
  • 规格：
    ￥2328
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    Pml
  • Uniprot No.：
    Q60953
  • 别名：
    Protein PML
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 来源：
    E.coli
  • 分子量：
    28.1 kDa
  • 表达区域：
    60-243aa
  • 氨基酸序列
    LRCPSCQAQAKCPKLLPCLHTLCSGCLEAPGLQCPICKAPGQADANGEALDNVFFESLQRRLAVFRQIVDAQAACTRCKGLADFWCFECEQLICSKCFEAHQWYLKHEARPLADLRDNSVSSFLDSTRKSNIFCSNTNHRNPALTDIYCRGCAKPLCCTCALLDRNHSHLHCDIGEEIQQWHEE
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged and C-terminal Myc-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

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Pml Proteins

靶点详情

  • 功能：
    Functions via its association with PML-nuclear bodies (PML-NBs) in a wide range of important cellular processes, including tumor suppression, transcriptional regulation, apoptosis, senescence, DNA damage response, and viral defense mechanisms. Acts as the scaffold of PML-NBs allowing other proteins to shuttle in and out, a process which is regulated by SUMO-mediated modifications and interactions. Positively regulates p53/TP53 by acting at different levels (by promoting its acetylation and phosphorylation and by inhibiting its MDM2-dependent degradation). Regulates phosphorylation of ITPR3 and plays a role in the regulation of calcium homeostasis at the endoplasmic reticulum. Regulates RB1 phosphorylation and activity. Acts as both a negative regulator of PPARGC1A acetylation and a potent activator of PPAR signaling and fatty acid oxidation. Regulates translation of HIF1A by sequestering MTOR, and thereby plays a role in neoangiogenesis and tumor vascularization. Regulates PER2 nuclear localization and circadian function. Cytoplasmic PML is involved in the regulation of the TGF-beta signaling pathway. Required for normal development of the brain cortex during embryogenesis. Plays a role in granulopoiesis or monopoiesis of myeloid progenitor cells. May play a role regulating stem and progenitor cell fate in tissues as diverse as blood, brain and breast. Shows antiviral activity towards lymphocytic choriomeningitis virus (LCMV) and the vesicular stomatitis virus (VSV).
  • 基因功能参考文献：
    1. Promyelocytic leukemia protein Pml -/- cells showed a higher proliferation rate, compared to Pml +/+ cells. PMID: 29302031
    2. PML protein organizes heterochromatin domains where it regulates histone H3.3 deposition by ATRX and DAXX. PMID: 28341773
    3. data support a model in which activation of myogenic differentiation results in PML NB loss, chromatin reorganization and DAXX relocalization, and provides a paradigm for understanding the consequence of PML loss in other cellular contexts, such as during cancer development and progression PMID: 28358373
    4. a regulatory role of ZNF451-1 in fine-tuning physiological PML levels PMID: 27343429
    5. PML loss promotes tumor development, providing a growth advantage to tumor cells that use autophagy as a cell survival strategy during stress conditions. PMID: 27545895
    6. The data demonstrate a dual role of PML in protection and recovery after brain injury. PMID: 27468695
    7. This study designates PML protein and PML-NBs to be major cellular components involved in the control of Herpes simplex virus 1 (HSV-1) latency, probably during the entire life of an individual. PMID: 27618691
    8. Study found that Promyelocytic leukemia protein (PML) is broadly expressed across the gray matter, with the highest levels in the cerebral and cerebellar cortices. PML bodies are broadly involved in activity-dependent nuclear phenomena in adult neurons PMID: 25956166
    9. These findings suggest that the UBC9/PML/RNF4 axis plays a critical role as an important SUMO pathway in cardiac fibrosis. Modulating the protein levels of the pathway provides an attractive therapeutic target for the treatment of cardiac fibrosis and heart failure. PMID: 28143738
    10. PML contributes to the intrinsic restriction of HIV-1 infections in a cell type-dependent manner. PMID: 26703718
    11. both the PML-RARA-driven competitive transplantation advantage and development of acute promyelocytic leukemia (APL) required DNMT3A PMID: 26595813
    12. PML IV enhances global SUMO-1 conjugation, particularly that of p53, resulting in p53 stabilization and activation. PMID: 26578773
    13. our findings challenge the predominant model in the field and we propose that PML/RARA initiates leukemia by subtly shifting cell fate decisions within the promyelocyte compartment. PMID: 26088929
    14. DNA-binding-defective PML/RARA mutants could not repress the transcription of retinoic acid regulated genes. PMID: 25119106
    15. pRB can interact with PML specifically during senescence, suggesting that signaling events in senescence regulate assembly of PML and pRB to establish heterochromatin and create a permanent cell cycle arrest. PMID: 24351540
    16. Data suggest that acute promyelocytic leukemia (APL) differentiation is a default program triggered by clearance of promyelocytic leukemia-retinoic acid receptor alpha fusion oncoprotein PML/RARA-bound promoters. PMID: 25258343
    17. Our results add PML/TRIM19 to the growing list of TRIM proteins implicated in both intrinsic and innate immunity. PMID: 24586174
    18. Oxidized PML spherical meshes recruit UBC9 and enhances PML sumoylation. PMID: 24637324
    19. ORF61 encoded the viral ribonucleotide reductase large subunit is a second PML-modifying protein. PMID: 24371073
    20. Activation of a promyelocytic leukemia-tumor protein 53 axis underlies acute promyelocytic leukemia cure. PMID: 24412926
    21. Promyelocytic leukemia protein is a cell-intrinsic factor to inhibit parvovirus DNA replication. PMID: 24198403
    22. PML and CycG2 mutually influence each other's functions following ionizing radiation. PMID: 23656780
    23. Loss of PML gene from male mutant p53 mice resulted in reduced survival, associated with a high prevalence of soft tissue sarcomas; suggesting that in males, cooperation between these pathways drove a more aggressive disease. PMID: 23656786
    24. Data indicate a role of promyelocytic leukemia protein (PML) in the regulation of metabolism and energy balance. PMID: 23986437
    25. PML-RARalpha co-operates with Sox4 in acute myeloid leukemia development in mice. PMID: 23144197
    26. role of PML in regulating cell adhesion, morphology, proliferation and migration PMID: 23555679
    27. PML deficiency facilitates genomic instability and promotes HBsAg-related hepatocarcinogenesis, which also involves androgen and lipid metabolism. PMID: 23620081
    28. PML protein and p53 reciprocally positively regulated each other during CPT-induced DNA damage, both of which were dependent on presenilin. PMID: 23306558
    29. PML knockout mice present impaired cognitive function and reduced anxiety-related behavior. PMID: 23279884
    30. These data are consistent with a model in which murine gammaherpesvirus 68 ORF75c could mediate direct ubiquitination of PML resulting in its degradation by the proteasome. PMID: 23541081
    31. Tumor suppressor promyelocytic leukemia protein selectively activates NLRP3 inflammasome. PMID: 23430110
    32. a unique role for PML in mTOR and EGFR inhibitor resistance and provide a strong rationale for a combination therapeutic strategy to overcome it. PMID: 23440206
    33. the tumor suppressor PML acts as an important barrier in liver carcinogenesis and HCV-dependent liver pathology PMID: 22984515
    34. SUMO interacting motif is dispensable for PML SUMOylation and interaction with RNF4 but is required for efficient PML ubiquitination, recruitment of proteasome components within NBs and proteasome-dependent degradation of PML in response to AsO PMID: 23028697
    35. PML protein interacts with Moloney murine leukemia virus integrase and reverse transcriptase during Moloney murine leukemia virus infection. PMID: 22685230
    36. Contribution of the C-terminal regions of promyelocytic leukemia protein (PML) isoforms II and V to PML nuclear body formation. PMID: 22773875
    37. A PML-PPAR-delta pathway for fatty acid oxidation regulates hematopoietic stem cell maintenance. PMID: 22902876
    38. PML and TBX2 act in an autoregulatory loop to control the effective execution of the senescence program. PMID: 22002537
    39. a model in which Pin1 promotes PML degradation in an ERK2-dependent manner. PMID: 22033920
    40. a novel functional connection between PML and the homologous recombination-mediated repair machinery PMID: 21998700
    41. PML may limit fat accumulation by suppressing the differentiation of preadipocytes into adipocytes. PMID: 21846906
    42. Setdb1 performs dual, but inseparable, functions at PML-NBs to maintain the structural integrity of PML-NBs and to control PML-NB-associated genes transcriptionally PMID: 21921037
    43. Neither Daxx nor PML, the main players of ND10-based immunity, are required for the block to viral gene expression in the S/G2 phase. PMID: 21832009
    44. study demonstrates a functional role for Pml in maintaining a specific open chromatin conformation of the Oct4 promoter region for its constant expression in stem cells PMID: 21360626
    45. The authors find that combined inactivation of Pml and Tsc2 results in aberrant TORC1 activity both in pre-tumoural kidneys as well as in kidney lesions. PMID: 21387562
    46. a key component for the differentiation of myeloid progenitor cells to macrophages PMID: 21427174
    47. PML functions as a positive regulator of IFNgamma signaling PMID: 21115099
    48. show that AXIN interacts with PML in vivo, and further that AXIN, PML and p53 form a ternary complex PMID: 21057547
    49. PML is induced by interferon, leading to a marked increase of expression of PML isoforms and the number of PML nuclear bodies [Review] PMID: 21198351
    50. expression of PML isoform PMLIV or PMLIVa, which is missing exon 5, inhibited rabies viral mRNA and protein synthesis, leading to a reduction in viral replication PMID: 20702643

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  • 亚细胞定位：
    Nucleus. Nucleus, nucleoplasm. Cytoplasm. Nucleus, PML body. Nucleus, nucleolus. Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Early endosome membrane; Peripheral membrane protein; Cytoplasmic side.
  • 数据库链接：

    KEGG: mmu:18854

    STRING: 10090.ENSMUSP00000082816

    UniGene: Mm.392123

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