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  4. Recombinant Mouse Piwi-like protein 1 (Piwil1), partial

Recombinant Mouse Piwi-like protein 1 (Piwil1), partial

  • 中文名称：
    小鼠Piwil1重组蛋白
  • 货号：
    CSB-YP867560MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Piwil1重组蛋白
  • 货号：
    CSB-EP867560MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Piwil1重组蛋白
  • 货号：
    CSB-EP867560MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Piwil1重组蛋白
  • 货号：
    CSB-BP867560MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Piwil1重组蛋白
  • 货号：
    CSB-MP867560MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Piwil1
  • Uniprot No.：
    Q9JMB7
  • 别名：
    Piwil1; Miwi; Piwi-like protein 1; EC 3.1.26.-
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Endoribonuclease that plays a central role in postnatal germ cells by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Directly binds methylated piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Strongly prefers a uridine in the first position of their guide (g1U preference, also named 1U-bias). Not involved in the piRNA amplification loop, also named ping-pong amplification cycle. Acts as an endoribonuclease that cleaves transposon messenger RNAs. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Probable component of some RISC complex, which mediates RNA cleavage and translational silencing. Also plays a role in the formation of chromatoid bodies and is required for some miRNAs stability. Required to sequester RNF8 in the cytoplasm until late spermatogenesis; RNF8 being released upon ubiquitination and degradation of PIWIL1.
  • 基因功能参考文献：
    1. Unlike most other Tudor domains TDRD2 preferentially recognizes an unmethylated arginine-rich sequence from PIWIL1. PMID: 29118143
    2. MIWI (or PIWIL1) depletion had a smaller effect. In oocytes lacking PLD6 (or ZUCCHINI or MITOPLD), a mitochondrial nuclease/phospholipase involved in piRNA biogenesis in male germ cells, the piRNA level was decreased to 50% compared to wild-type. PMID: 28115634
    3. Stem cell protein Piwil1 plays an important role in regulating stem-like properties of endometrial cancer cells. PMID: 26506848
    4. PIWIL1 regulates neuronal polarization and radial migration partly via modulating the expression of microtubule-associated proteins. PMID: 26104391
    5. Results show that HIWI is highly expressed in high-grade squamous intraepithelial lesions and cervical cancer tissues suggesting its participation in the carcinogenesis of cervical cancer. PMID: 25119492
    6. Miwi protein might be an important functional protein in murine folliculogenesis PMID: 23244769
    7. This work identifies an epigenetic regulation mechanism for the spatio-temporal expression of mouse Miwi during spermatogenesis. PMID: 22661915
    8. Co-immunoprecipitation assay in vivo and in vitro confirmed MIWI is a bona fide partner of STK31 in mice testes. PMID: 22205278
    9. Miwi is a small RNA-guided RNase (slicer) that requires extensive complementarity for target cleavage in vitro PMID: 22121019
    10. PIWI proteins function in meiosis during spermatogenesis. PMID: 21539824
    11. Mouse homologue of the Piwi Protein recognizes the 2'-O-Methylated 3'-End of piRNA by the PAZ Domain. PMID: 21237665
    12. MIWI, a murine Piwi protein, binds a previously uncharacterized class of approximately 29-30-nucleotide RNAs that are highly abundant in testes PMID: 16751776
    13. A functional interplay between KIF17b and MIWI might be needed for the loading of haploid RNAs in the chromatoid body. PMID: 16787948
    14. Piwi-interacting RNAs are fully modified by 2'-O-methylation at their 3' termini: this observation may provide a clue to the biogenesis and function of piRNAs in spermatogenesis PMID: 17384646
    15. 3' termini of Piwi-interacting RNAs are 2'-O-methylated; this modification may have important implications for the biogenesis and function of piRNAs PMID: 17384647

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  • 亚细胞定位：
    Cytoplasm. Note=Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis. Also present in chromatoid body.
  • 蛋白家族：
    Argonaute family, Piwi subfamily
  • 组织特异性：
    Expressed in brain. Expressed in testis, specifically in spermatocytes (at protein level). Only detected in germ lineage cells of adult testis. Expressed in male gonads 2 weeks after birth at the initiation of spermatogenesis, but not expressed in female
  • 数据库链接：

    KEGG: mmu:57749

    STRING: 10090.ENSMUSP00000083222

    UniGene: Mm.272720

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