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  4. Recombinant Mouse Phospholipid hydroperoxide glutathione peroxidase, mitochondrial (Gpx4), partial

Recombinant Mouse Phospholipid hydroperoxide glutathione peroxidase, mitochondrial (Gpx4), partial

  • 中文名称：
    小鼠Gpx4重组蛋白
  • 货号：
    CSB-YP524814MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Gpx4重组蛋白
  • 货号：
    CSB-EP524814MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Gpx4重组蛋白
  • 货号：
    CSB-EP524814MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Gpx4重组蛋白
  • 货号：
    CSB-BP524814MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Gpx4重组蛋白
  • 货号：
    CSB-MP524814MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Gpx4
  • Uniprot No.：
    O70325
  • 别名：
    Gpx4Phospholipid hydroperoxide glutathione peroxidase; PHGPx; EC 1.11.1.12; Glutathione peroxidase 4; GPx-4; GSHPx-4
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 表达区域：
    -
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Essential antioxidant peroxidase that directly reduces phospholipid hydroperoxide even if they are incorporated in membranes and lipoproteins. Can also reduce fatty acid hydroperoxide, cholesterol hydroperoxide and thymine hydroperoxide. Plays a key role in protecting cells from oxidative damage by preventing membrane lipid peroxidation. Required to prevent cells from ferroptosis, a non-apoptotic cell death resulting from an iron-dependent accumulation of lipid reactive oxygen species. The presence of selenocysteine (Sec) versus Cys at the active site is essential for life: it provides resistance to overoxidation and prevents cells against ferroptosis. The presence of Sec at the active site is also essential for the survival of a specific type of parvalbumin-positive interneurons, thereby preventing against fatal epileptic seizures. May be required to protect cells from the toxicity of ingested lipid hydroperoxides. Required for normal sperm development and male fertility. Essential for maturation and survival of photoreceptor cells. Plays a role in a primary T-cell response to viral and parasitic infection by protecting T-cells from ferroptosis and by supporting T-cell expansion. Plays a role of glutathione peroxidase in platelets in the arachidonic acid metabolism. Reduces hydroperoxy ester lipids formed by a 15-lipoxygenase that may play a role as down-regulator of the cellular 15-lipoxygenase pathway.; Specifically able to suppress the production of leukotriene and prostaglandin in response to several stimuli by reducing fatty acid hydroperoxide.; Specifically required to prevent mitochondrial cell death by mediating reduction of cardiolipin hydroperoxide. Also required for normal sperm development and male fertility.; Required for male fertility by stabilizing the condensed chromatin in sperm nuclei.
  • 基因功能参考文献：
    1. paternal acetylated histones and acrosomal nuclear GPx4 are directly involved in fertilization. PMID: 28802016
    2. Gpx4 was dispensable for the maintenance of cellular health and function of POMC neurons, even in mice exposed to obesogenic conditions. In contrast, HFHS-fed mice with Gpx4 deletion from AgRP neurons displayed increased body adiposity. Gpx4 deletion from dopaminergic neurons induced anxiety behavior, and diminished spontaneous locomotor activity when DJ-1 was co-deleted. PMID: 28627418
    3. After tamoxifen treatment to ablate Gpx4, Gpx4BIKO mice developed impairment in hippocampus dependent spatial learning and memory function which was associated with neurodegeneration. Consistent with its role as a regulator of ferroptosis, cognitive impairment and neurodegeneration in Gpx4BIKO mice was associated with elevated lipid peroxidation, ERK(1/2) activation and overt neuroinflammation. PMID: 28212525
    4. The structure of the cytoplasmic isoform of mouse phospholipid hydroperoxide glutathione peroxidase (O70325-2 GPx4) with selenocysteine 46 mutated to cysteine is reported. PMID: 27710939
    5. ferroptosis regulator Gpx4 is critical for hepatocyte survival and proper liver function, and that vitamin E can compensate for its loss by protecting cells against deleterious lipid peroxidation. PMID: 27262435
    6. These data suggest that systemic inactivation of the Alox15 gene normalizes the reduced fertility of male Sec46Ala-Gpx4(+/-) mice by improving the motility of their sperm. If these data can be confirmed in humans, ALOX15 inhibitors might counteract male infertility related to GPX4 deficiency. PMID: 27634046
    7. Phospholipid hydroperoxide glutathione peroxidase is involved in the maintenance of male fertility under cryptorchidism in mice PMID: 26050606
    8. dramatic motor neuron degeneration and paralysis induced by Gpx4 ablation suggest that ferroptosis inhibition by GPX4 is essential for motor neuron health and survival in vivo PMID: 26400084
    9. The lack of catalytic activity is the major reason for the embryonic lethality of Gpx4 knockout mice. PMID: 25313597
    10. Homozygous expression of Gpx4 with a targeted substitution of selenocysteine to serine causes early embryonic death as expected but, unexpectedly, male subfertility in heterozygous mice. PMID: 25922076
    11. GPx4 suppresses the increase in the VEGF-A protein level, which occurs during the development of pathological CNV, thus partly explaining the protective effect of GPx4 against CNV. PMID: 24897344
    12. Inducible disruption of Gpx4 causes acute renal failure and early death in an Alox15-independent manner PMID: 25402683
    13. A membrane glycoprotein GPX4 was shown to play a significant role in gamete interactions. PMID: 24191733
    14. Rat Gpx4 is a moonlighting protein that functions both as a peroxidase, as well as a structural protein. PMID: 10464096
    15. GPx4-overexpressing mice showed no free fatty acid induced increase in reactive oxygen species and lipid peroxidation PMID: 23695217
    16. Activity of cutaneous GPx4 is linked to the regulation of COX-2 and hair follicle morphogenesis and provides insight into the function of individual selenoprotein activity in maintaining cutaneous homeostasis. PMID: 23364477
    17. enzymatic epididymal salvage response is sufficient to maintain full fertility of double GPx4 and GPx5 KO males whatever their age, crossed with young WT female mice PMID: 22719900
    18. Tam treatment also significantly elevated apoptosis in Gpx4(f/f)/Cre mice. Moreover, tam-treated Gpx4(f/f)/Cre mice showed neuronal loss in the hippocampus region and had increased astrogliosis PMID: 22401858
    19. GPx4 is a critical antioxidant enzyme for the maturation and survival of photoreceptor cells. PMID: 22207760
    20. the absence of nuclear GPx4 leads to sperm nuclear matrix/chromatin instability that may negatively affect embryo development PMID: 21618532
    21. Phospholipid hydroperoxide glutathione peroxidase gene is regulated via an estrogen and estrogen receptor signaling in cultured mouse fetuses PMID: 21717270
    22. Data indicate that both catalytic efficiency and the expression level of GPx4 control the balance between cell survival and proliferation. PMID: 21402720
    23. Absence of glutathione peroxidase 4 affects tumor angiogenesis through increased 12/15-lipoxygenase activity. PMID: 20234819
    24. Data conclude that GPx4 is a selenoenzyme modulating interneuron function and parvalbumin expression. PMID: 19890015
    25. These data suggest a role of GPx4 in neurodegenerative diseases through its function in removal of lipid hydroperoxides. PMID: 19769463
    26. in three models of oxidative damage-induced retinal degeneration, increased expression of Gpx4 provided strong protection of retinal structure and function PMID: 18823256
    27. analysis of mRNA in testis and other tissues PMID: 12152197
    28. tissue distribution of alternatively spliced snGPx PMID: 12427732
    29. observations establish glutathione peroxidase 4 as an essential antioxidant enzyme in mice and suggest that it performs broad functions as a component of the mammalian antioxidant network PMID: 12566075
    30. Early embryonic lethality caused by targeted disruption of the PHGPx gene. PMID: 12745070
    31. Functional characterization of cis- and trans-regulatory elements involved in expression of phospholipid hydroperoxide glutathione peroxidase. PMID: 12888488
    32. mouse embryonic fibroblasts from Gpx4(+/-) mice are more sensitive to oxidative stress PMID: 14572612
    33. Gpx4 plays a role in vivo in the mechanism of apoptosis induced by oxidative stress that most likely occurs through oxidative damage to mitochondrial phospholipids such as cardiolipin PMID: 15496407
    34. TGR and GPx4 can serve as a novel disulfide bond formation system and contain a catalytic selenocysteine consistent with the role of selenium in male reproduction PMID: 15901730
    35. nPHGPx, by acting as a protein thiol peroxidase in vivo, contributes to the structural stability of sperm chromatin PMID: 16107710
    36. Gpx4 haploid insufficiency reduced Gpx4 activities and/or selenium concentrations, but had no effect on pro-oxidant-induced protein oxidation in various tissues. PMID: 16246897
    37. phospholipid hydroperoxide glutathione peroxidase isoforms have roles in murine embryogenesis PMID: 16684775
    38. Therefore, our results indicate that decreased ATP production under oxidative stress is primarily due to reduced mitochondrial membrane potential and overexpression of Gpx4 maintains mitochondrial membrane potential under oxidative stress. PMID: 17395155
    39. These results indicate that PHGPx exhibits a cell- and tissue-specific expression pattern in mice. PMID: 17503194
    40. Mitochondrial PHGPx expression is tightly regulated in pachytene spermatocytes, with any increase in PHGPx expression resulting in damage to spermatogenesis and eventual loss of haploid cells PMID: 17540721
    41. lifelong reduction in Gpx4 increased life span and reduced/retarded age-related pathology most likely through alterations in sensitivity of tissues to apoptosis PMID: 17895430
    42. adult lung fibroblasts deficient in GPx4 may have upregulated compensatory mechanisms to deal with the highly oxidized environment in which they developed. PMID: 18206984
    43. Neuron-specific GPx4 depletion caused neurodegeneration in vivo and ex vivo, highlighting the importance of this pathway in neuronal cells. PMID: 18762024
    44. mitochondrial GPx4 confers the vital role of selenium in mammalian male fertility PMID: 19417079
    45. Transgenic mouse models for the vital selenoenzymes cytosolic thioredoxin reductase, mitochondrial thioredoxin reductase and glutathione peroxidase 4. PMID: 19433132
    46. Liver apoptosis and Cyt c release are suppressed in Tg(GPX4+/0) mice but exacerbated in Gpx4+/- mice PMID: 19447173
    47. depletion of GPx4 in spermatocytes causes severe abnormalities in spermatozoa PMID: 19783653

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  • 亚细胞定位：
    [Isoform Mitochondrial]: Mitochondrion.; [Isoform Cytoplasmic]: Cytoplasm.; [Isoform Nuclear]: Nucleus.
  • 蛋白家族：
    Glutathione peroxidase family
  • 组织特异性：
    Widely expressed with the highest levels in testis, heart, cerebrum, ileum, stomach, liver, jejunum and epididymis. Expressed primarily in testis and sperm midpiece (at protein level). Expressed in brain (at protein level). Expressed in heart, liver and k
  • 数据库链接：

    KEGG: mmu:625249

    UniGene: Mm.332810

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