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  4. Recombinant Mouse Phosphatidylinositol 3-kinase catalytic subunit type 3 (Pik3c3), partial

Recombinant Mouse Phosphatidylinositol 3-kinase catalytic subunit type 3 (Pik3c3), partial

  • 中文名称：
    小鼠Pik3c3重组蛋白
  • 货号：
    CSB-YP754257MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Pik3c3重组蛋白
  • 货号：
    CSB-EP754257MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Pik3c3重组蛋白
  • 货号：
    CSB-EP754257MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Pik3c3重组蛋白
  • 货号：
    CSB-BP754257MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Pik3c3重组蛋白
  • 货号：
    CSB-MP754257MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Pik3c3
  • Uniprot No.：
    Q6PF93
  • 别名：
    Pik3c3; Vps34; Phosphatidylinositol 3-kinase catalytic subunit type 3; PI3-kinase type 3; PI3K type 3; PtdIns-3-kinase type 3; EC 2.7.1.137; Phosphoinositide-3-kinase class 3
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. As part of PI3KC3-C1, promotes endoplasmic reticulum membrane curvature formation prior to vesicle budding. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for transport from early to late endosomes.
  • 基因功能参考文献：
    1. In mouse liver, Vps34 inactivation mildly dampens autophagy, limiting substrate availability for mitochondrial respiration and reducing gluconeogenesis. PMID: 29180704
    2. dendritic cells from Vps34-deficient mice have a partially activated phenotype, spontaneously produce cytokines, and exhibit enhanced activity of the classic MHC class I and class II antigen-presentation pathways and displayed a defect in the homeostatic maintenance of splenic CD8alpha(+) dendritic cells and in the capacity of these cells to cross-present cell corpse-associated antigens to MHC class I-restricted T cells. PMID: 28716903
    3. Results demonstrate that Vps34 is essential for proper myelination by Schwann cells. Depletion of PI3P leads to enlarged late endosomal/lysosomal vacuoles and suppressed trafficking in Schwann cells. Suppressed endosomal trafficking likely causes changes in the abundance and post-translational modification of ErbB2/3, a signaling defect that may contribute to arrested myelination in Vps34 deficient nerves. PMID: 28617998
    4. Phosphatidylinositol-3-phosphate is light-regulated, and Vps34 is essential for survival of retinal rod photoreceptor cells. PMID: 27245220
    5. This study reveals NRBF2 as a critical molecular switch of PtdIns3K and autophagy activation, and its on/off state is precisely controlled by MTORC1 through phosphorylation. PMID: 28059666
    6. This study uncovers a dual role for Vps34 as a regulator of platelet production by megakaryocytes and as an unexpected regulator of platelet activation and arterial thrombus formation dynamics. PMID: 28903944
    7. E. chaffeensis secretes Etf-1 to induce autophagy to repurpose the host cytoplasm and capture nutrients for its growth through RAB5 and class III PtdIns3K, while avoiding autolysosomal killing. PMID: 27541856
    8. p300-dependent VPS34 acetylation/deacetylation is the physiological key to VPS34 activation, which controls the initiation of canonical autophagy and of non-canonical autophagy in which the upstream kinases of VPS34 can be bypassed. PMID: 28844862
    9. Vps34 has a previously unknown role in regulating Rab7 activity and late endosomal trafficking. PMID: 27793976
    10. PIK3C3 was strongly expressed in the axon of cortical neurons during brain development. PMID: 26242203
    11. provide the novel evidence that GADD45A inhibits autophagy via impairing the BECN1-PIK3C3 complex formation PMID: 26636486
    12. PAQR3 controls autophagy by integrating AMPK signaling to enhance ATG14L-associated PI3K activity PMID: 26834238
    13. Depletion of hepatic Vps15, the regulatory subunit of class III PI3K, increases insulin sensitivity and Akt signaling. PMID: 26387534
    14. Study identifies a key role of Cul3-KLHL20 in autophagy termination by controlling autophagy-dependent turnover of ULK1 and VPS34 complex subunits and reveals the pathophysiological functions of this autophagy termination mechanism. PMID: 26687681
    15. Class III PI3K plays a central role in the transition of cardiac hypertrophy to heart failure via a prolonged activation of autophagy. PMID: 25851780
    16. These data reveal a key role for NRBF2 in the assembly of the specific Atg14L-Beclin 1-Vps34-Vps15 complex for autophagy induction. PMID: 24849286
    17. Vps34 is a main phosphatidylinositol 3-phosphate source for constitutive PIKfyve functionality. PMID: 25619930
    18. a novel association between deletion of Pik3c3 and muscular dystrophy. PMID: 24726497
    19. Our studies indicate that while Vps34 is the main PI3P source during autophagy, class II PI3Ks also significantly contribute to PI3P generation and regulate AP biogenesis. PMID: 24098492
    20. Conditional Vps34 knockout in mouse kidney PTC causes vacuolation and intracellular sequestration of megalin. PMID: 23621784
    21. Vps34 is a major regulator of endolysosomal pathways in podocytes and underline the fundamental roles of endocytosis and fluid-phase uptake for the maintenance of the glomerular filtration barrier. PMID: 23492732
    22. our study reveals a critical role for Vps34 in autophagy and for the peripheral homeostasis and function of T lymphocytes. PMID: 23596309
    23. Acetylated hsp70 and KAP1-mediated Vps34 SUMOylation is required for autophagosome creation in autophagy. PMID: 23569248
    24. the physiological role of Atg5 and Pik3c3 in quality control and development of the lens, respectively. PMID: 23479732
    25. Mouse skeletal muscle fiber-type-specific macroautophagy and muscle wasting are regulated by a Fyn/STAT3/Vps34 signaling pathway. PMID: 22745922
    26. These results demonstrate an indispensable role for mVps34 in the trafficking of intracellular vesicles to protect the normal cellular metabolism, structure, and function of podocytes. PMID: 23291473
    27. Study reveals an intricate molecular regulation of Vps34 complexes by AMPK in nutrient stress response and autophagy. PMID: 23332761
    28. Data show that Vps34 was dispensable for T-cell development but important for the survival of naive T cells. PMID: 22592798
    29. conclude that Slamf1 recruits a subset of Vps34-associated proteins, which is involved in membrane fusion and NOX2 regulation PMID: 22493499
    30. Vps34 plays an essential role in regulating functional autophagy and is indispensable for normal liver and heart function. PMID: 22308354
    31. Pik3c3 is essential for central nervous system neuronal homeostasis and Pik3c3 deletion; CaMKII-Cre transgenic mouse is a useful model for studying pathological changes in progressive forebrain neurodegeneration. PMID: 20955765
    32. mTOR signaling is drastically reduced in Pik3c3 null embryos, which could be a major contributor to the observed proliferation and embryogenesis defects PMID: 21283715
    33. A conditional null allele of the Pik3c3 gene was generated and specifically deleted it in postmitotic sensory neurons. PMID: 20562532
    34. Deletion of PIK3C3/Vps34 in sensory neurons causes rapid neurodegeneration by disrupting the endosomal but not the autophagic pathway. PMID: 20439739
    35. Class III PI3K Vps34 is responsible for the synthesis of PtdIns(3)P on phagosomes containing either S aureus or E coli. PtdIns(3)P binding to p40(phox) is important for CD18-dependent activation oxidase activation in response to S aureus and E coli PMID: 18755982

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  • 亚细胞定位：
    Midbody. Late endosome. Cytoplasmic vesicle, autophagosome.
  • 蛋白家族：
    PI3/PI4-kinase family
  • 数据库链接：

    KEGG: mmu:225326

    STRING: 10090.ENSMUSP00000111479

    UniGene: Mm.194127

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