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  4. Recombinant Mouse Myosin light chain 1/3, skeletal muscle isoform (Myl1)

Recombinant Mouse Myosin light chain 1/3, skeletal muscle isoform (Myl1)

  • 中文名称：
    小鼠Myl1重组蛋白
  • 货号：
    CSB-YP015305MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Myl1重组蛋白
  • 货号：
    CSB-EP015305MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Myl1重组蛋白
  • 货号：
    CSB-EP015305MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Myl1重组蛋白
  • 货号：
    CSB-BP015305MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Myl1重组蛋白
  • 货号：
    CSB-MP015305MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Myl1
  • Uniprot No.：
    P05977
  • 别名：
    Myl1; Mylf; Myosin light chain 1/3; skeletal muscle isoform; MLC1/MLC3; MLC1F/MLC3F; Myosin light chain alkali 1/2; Myosin light chain A1/A2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-188
  • 氨基酸序列
    APKKDVKKP AAAPAPAPAP APAPAKPKEE KIDLSAIKIE FSKEQQEDFK EAFLLFDRTG ECKITLSQVG DVLRALGTNP TNAEVKKVLG NPSNEEMNAK KIEFEQFLPM MQAISNNKDQ GGYEDFVEGL RVFDKEGNGT VMGAELRHVL ATLGEKMKEE EVEALLAGQE DSNGCINYEA FVKHIMSV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Non-regulatory myosin light chain required for proper formation and/or maintenance of myofibers, and thus appropriate muscle function.
  • 基因功能参考文献：
    1. aPKCzeta is required for the cellular organization of acto-non-muscle myosin II (NMII) cytoskeleton, for proper cell adhesion and directed cell migration. PMID: 27541056
    2. This study provides an additional mechanistic link between Angiotensin II and vasoconstriction via SFK-enhanced MLC phosphorylation in smooth muscle cells. PMID: 26011449
    3. The present work further established the role of MuRF1 in muscle atrophy and for the first time shows that MuRF1 plays a role in muscle fatigue and twitch potentiation. PMID: 20625437
    4. Targeted deletion of the MLC1f/3 downstream enhancer results in precocious MLC expression and mesoderm ablation. This suggests that the enhancer and its flanking sequences in clude negative regulatory elements as well as positive ones. PMID: 11884037
    5. myotome pairs are transiently left-right asymmetric, with higher expression of alpha-skeletal actin and myosin light chain 3F (MLC3F) on the left side between embryonic day 9.5-10.25. PMID: 15499557
    6. Using double reporter transgenes study demonstrates that each enhancer can activate both Mlc1f and Mlc3f promoters in vivo, revealing enhancer sharing rather than exclusive enhancer-promoter interactions. PMID: 18062958
    7. MLC1f expression is reversely related to the proliferation of muscle cells. PMID: 18957633

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  • 数据库链接：

    KEGG: mmu:17901

    STRING: 10090.ENSMUSP00000027151

    UniGene: Mm.1000

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