Recombinant Mouse MKL/myocardin-like protein 1 (Mkl1), partial

1. Study have demonstrated a stress-dependent p38MAPK/MK2-driven phosphorylation of two defined, well conserved serine residues of MRTF-A in various cultured cell lines as well as in vitro. These observation suggests that the modulation of MRTF-A activity by MK2-mediated phosphorylation could represent a novel crosstalk between myogenic and stress signaling. PMID: 27492266
2. these data indicate that Emerin, a conserved nuclear lamina protein, couples extracellular matrix mechanics and SRF-Mkl1-dependent transcription. PMID: 28576971
3. Among a group of tumor cells, there is correlation between activation of the MRTF-dependent transcription and activated FAK-dependent regulation of cell migration. PMID: 27708220
4. Here, the authors show that Rho-dependent MRTF phosphorylation reflects its nuclear accumulation and dissociation from G-actin, and identify multiple sites for MRTF phosphorylation, which contribute to transcriptional activation. PMID: 27304076
5. BRG1 promotes transcription of endothelial Mrtfa and Mrtfb, which elevates expression of SRF and SRF target genes that establish embryonic capillary integrity. PMID: 28729363
6. knockdown of MKL1 induces a significant increase in the transcriptional activity of PPARgamma target genes and MKL1 interacts with PPARg, suggesting that SRF and MKL1 independently inhibit brown adipogenesis and that MKL1 exerts its effect mainly by modulating PPARgamma activity PMID: 28125644
7. further found that hypericin ameliorates inflammatory response by suppressing MKL1, which is the essential cofactor of p65 during the transcription process. In an Abeta injection AD mouse model, animals orally administrated hypericin (50 mg/kg) for seven days significantly decreased pro-inflammatory cytokines expression and NO production in hippocampus, meanwhile, hypericin improved oAbeta42-induced learning and memory... PMID: 27825966
8. Study demonstrates that WH2 domains activate MRTF-A and contribute to target gene regulation by a competitive mechanism, independently of their role in actin filament formation. PMID: 26976641
9. The transcriptional co-activator MRTF-A was activated by sphingosine-1-phosphate as assessed by its nuclear accumulation and induction of a RhoA/MRTF-A luciferase reporter. PMID: 27094722
10. MRTF-A regulates liver fibrosis by epigenetically tuning the TGF-beta signaling pathway in HSCs PMID: 26693892
11. Exploration of the molecular causes of enhanced cardiac hypertrophy revealed significant activation of beta-catenin/GSK-3beta signaling, whereas MAPK and MKL1/serum-response factor pathways were inhibited. PMID: 26719331
12. MRTF-A is a critical for epithelial to mesenchymal transition and can be stereoselectively inhibited by CCG-1423. PMID: 26295164
13. MKL1 plays a significant role in mediating the fibrotic response to bleomycin injury. Loss of MKL1 attenuated early neutrophil influx, as well as myofibroblast-mediated remodeling. PMID: 25885656
14. MRTF-A/B depletion results in an increase in the cell surface expression of ICAM-1 and interactions between HAoECs and leukocytes PMID: 26024305
15. knockdown of MRTF-A synthesis abolishes the systemic sclerosis myofibroblast enhanced basal contractility and synthesis of type I collagen and inhibits the matricellular profibrotic protein, connective tissue growth factor (CCN2/CTGF PMID: 25955164
16. Either MRTF-A or MRTF-B is dispensable for cardiac development, whereas deletion of both causes a spectrum of abnormalities ranging from reduced cardiac contractility and adult onset heart failure to neonatal lethality accompanied by sarcomere disarray. PMID: 26386146
17. Ddx19 is an RNA export factor required for nuclear import of the SRF coactivator MKL1 PMID: 25585691
18. Expression analysis showed that MKL1 is highly expressed in human and mouse brains; results revealed that genetic variants in MKL1 might confer risk to schizophrenia. PMID: 25380769
19. Results found that MKL1 expression was upregulated during cell cycle arrest induced by a temperature switch in podocytes through p21 transcription activation suggesting a physiological roles in the maturation and development of podocytes. PMID: 25888165
20. These data support a central role of the SRF/MRTF pathway in the pathobiology of lung fibrosis. PMID: 25681733
21. MRTF-A promotes microvessel growth (via CCN1) and maturation (via CCN2), thereby enabling functional improvement of ischaemic muscle tissue PMID: 24910328
22. we data have unveiled a MRTF-A-containing complex that links ET-1 transactivation in endothelial cells to cardiac hypertrophy and fibrosis by Ang II. PMID: 25446178
23. the actin, myocardin-related transcription factors and serum response factor (actin-Mrtf-Srf) pathway is specifically downregulated in the muscle atrophy that is induced through disuse in mice. PMID: 25344251
24. These results identify SRF and its MRTF cofactors as major transcriptional regulators of endothelial cell junctional stability, guaranteeing physiological functions of the cerebral microvasculature. PMID: 26221020
25. TGF-beta stimulated the binding of MKL1 on the promoters of pro-fibrogenic genes and promoted the interaction between MKL1 and SMAD3 PMID: 26241940
26. MRTFA regulates renal fibrosis in diabetic nephropathy. PMID: 25349198
27. MKL1/MRTF-A, by coordinating key epigenetic alterations on CAM promoters, provides a critical link to hypoxia-induced endothelial malfunction and contributes to the pathogenesis of hypoxia-induced pulmonary hypertension. PMID: 25646298
28. Study identified a BMP7-controlled signaling and transcriptional circuit involving MRTFA, which enhances the development of beige adipocytes in white adipose tissue, resulting in protection from diet-induced obesity and insulin resistance. PMID: 25579684
29. Suggest role for nuclear RhoA signaling in MRTF-dependent gene expression in smooth muscle cells. PMID: 24906914
30. Myocardin-related transcription factor (MRTF)-A and MRTF-B (MKL1 and MKL2, respectively) are enriched in the perinuclear space of epicardial cells during development. PMID: 25516967
31. Data show that the binding of WIP to actin controls the actin dynamics MRTFA-SRF-Focal adhesion assembly signaling cascade. PMID: 24797074
32. Data indicate that RNA interference mediated loss of MKL1 (megakaryoblastic leukemia 1) drives adipocyte differentiation. PMID: 24569594
33. MKL1/2 and ELK4 co-regulate distinct serum response factor (SRF) transcription programs in macrophages. PMID: 24758171
34. TGF-beta1-induced epithelial-myofibroblast transition via MRTF-A signaling is regulated by cell adhesion and shape PMID: 24340092
35. MRTF-A knockdown leads to increase in S and G2/M populations and downregulation of cyclin-CDK inhibitors p27Kip1, p18Ink4c and 19Ink4d as well as upregulation of p21Waf1 and cyclin D1. PMID: 23656782
36. Isoxazole stimulates myofibroblast differentiation via induction of MRTF-A-dependent gene expression. PMID: 24082095
37. lamin-A/C-deficient and Lmna(N195K/N195K) mutant cells have impaired nuclear translocation and downstream signalling of MKL1, a myocardin family member that is pivotal in cardiac development and function PMID: 23644458
38. Activated mDia promoted rapid and reversible nuclear actin network assembly, subsequent MAL nuclear accumulation, and SRF activity. PMID: 23558171
39. Data suggest that MKL1 and MKL2 are expressed in megakaryocytes and platelets; megakaryocytes lacking expression of MKL1 and/or MKL2 have both defective megakaryocytopoiesis and thrombopoiesis. PMID: 22806889
40. A role for MAL in TNF signaling and implicate the MAL-SRF transcription module in regulating the proapoptotic Bcl-2 family network. PMID: 22185759
41. The cytoskeleton-associated factors integrin alpha5, Pkp2 and FHL1 were newly characterised as transcriptionally regulated actin-MAL-SRF target genes, and they were in part responsible for the impaired migration of cells harbouring activated MAL. PMID: 22223881
42. MRTF-A nuclear accumulation following stimulation with serum, actin drugs or acute mechanical stress is prevented within mechanically loaded, anchored matrices at tensional homeostasis. PMID: 21799516
43. MRTF-A is an important regulator of collagen synthesis in lung fibroblasts and exhibits a dependence on both SRF and Sp1 function to enhance collagen expression PMID: 22049076
44. structural, biochemical and cell biological evidence that MAL has a classical bipartite nuclear localization signal (NLS) in the N-terminal 'RPEL' domain containing Arg-Pro-X-X-X-Glu-Leu (RPEL) motifs was presented. PMID: 21964294
45. Promoter-reporter and chromatin immunoprecipitation experiments unraveled a SAP-dependent, SRF-independent interaction of MKL1 with the proximal promoter region of TNC. PMID: 21705668
46. Structure of a pentavalent G-actin*MRTF-A complex reveals how G-actin controls nucleocytoplasmic shuttling of a transcriptional coactivator. PMID: 21673315
47. Presents evidence that strongly suggests a dual role for MKL1 in oncogenic mechanisms, as a tumor-promoting or tumor-suppressing molecule. PMID: 20816842
48. Data show that MRTF-A contains an unusually long bipartite nuclear localisation signal embedded within the RPEL domain, that uses the importin (Imp)alpha/beta-dependent import pathway, and that import is inhibited by G-actin. PMID: 20818336
49. Expression of brain natriuretic peptide and other serum response factor-dependent fetal cardiac genes in response to acute mechanical stress was blunted in mice lacking Mrtf-A. PMID: 20606005
50. MRTF-A regulates myofibroblast activation and fibrosis in response to the renin-angiotensin system and post-myocardial infarction remodeling. PMID: 20558820

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KEGG: mmu:223701

STRING: 10090.ENSMUSP00000105207

UniGene: Mm.439814