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  4. Recombinant Mouse Keratin, type II cytoskeletal 8 (Krt8)

Recombinant Mouse Keratin, type II cytoskeletal 8 (Krt8)

  • 中文名称：
    小鼠Krt8重组蛋白
  • 货号：
    CSB-YP012574MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Krt8重组蛋白
  • 货号：
    CSB-EP012574MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Krt8重组蛋白
  • 货号：
    CSB-EP012574MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Krt8重组蛋白
  • 货号：
    CSB-BP012574MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Krt8重组蛋白
  • 货号：
    CSB-MP012574MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Krt8
  • Uniprot No.：
    P11679
  • 别名：
    Krt8; Krt2-8; Keratin; type II cytoskeletal 8; Cytokeratin endo A; Cytokeratin-8; CK-8; Keratin-8; K8; Type-II keratin Kb8
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-490
  • 氨基酸序列
    MSIRVTQKSY KMSTSGPRAF SSRSFTSGPG ARISSSSFSR VGSSSSSFRG SMGTGVGLGG FGGAGVGGIT AVTVNQSLLS PLKLEVDPNI QAVRTQEKEQ IKSLNNKFAS FIDKVRFLEQ QNKMLETKWS LLQQQKTSRS NMDNMFESYI NNLRRQLEAL GQEKLKLEAE LGNMQGLVED FKNKYEDEIN KRTEMENEFV LIKKDVDEAY MNKVELESRL EGLTDEINFL RQIHEEEIRE LQSQISDTSV VLSMDNSRSL DMDGIIAEVR AQYEDIANRS RAEAETMYQI KYEELQTLAG KHGDDLRRTK TEISEMNRNI NRLQAEIEAL KGQRASLEAA IADAEQRGEM AIKDAQTKLA ELEAALQRAK QDMARQLREY QELMNVKLAL DIEITTYRKL LEGEESRLES GMQNMSIHTK TTSGYSGGLS SSYGGLTSPG FSYGMSSFQP GFGSAGGSNT FSRTTKAVVV KKIETRDGKL VSESSDVVSK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
  • 基因功能参考文献：
    1. The authors found that keratin 8 suppressed toll-like receptor-induced nuclear factor (NF)-kappaB activation and interacted with the adaptor tumor necrosis factor (TNF) receptor-associated factor 6 (TRAF6) to prevent its polyubiquitination. PMID: 27586056
    2. K8/K18 interact with Notch1 and regulate Notch1 signalling activity during differentiation of the colonic epithelium. PMID: 28475172
    3. Mitochondria in K8 wild-type beta cells and MIN6 insulinoma cells overexpressing K8 and 18 are more stationary compared with mitochondria in keratin-deficient cells PMID: 28666985
    4. DRA mRNA levels were decreased by three- to fourfold and DRA protein was almost entirely lost in K8-/- cecum and proximal and distal colon compared with K8+/+. PMID: 27125276
    5. Upregulation of IL-22 in combination with a complete loss of its negative regulator IL-22BP, and increased downstream STAT3-signaling in K8(-/-) and K8(-/-)Apc(Min/+) colonic epithelia confirmed that the IL-22 pathway, important in inflammation, proliferation and tissue regeneration PMID: 27234655
    6. Krt8 deletion (Krt8(-/-)) in a mouse model of cystic fibrosis (F508del-Cftr mice) increased the levels of circulating markers of bone formation, corrected the expression of osteoblast phenotypic genes, promoted trabecular bone formation and improved bone mass and microarchitecture. Krt8 deletion corrected overactive NF-kappaB signaling & decreased Wnt-beta-catenin signaling induced by F508del-Cftr mutation in osteoblasts. PMID: 26769674
    7. Autoantibodies to several antigens were identified in 81% of K8-null male mice 8 mo or older. Similar autoantibodies were detected in aging K18-null male mice that had a related liver phenotype but normal colon compared with K8-null mice PMID: 26399787
    8. The regulation of the SCFA-MCT1-HMGCS2 axis is disrupted in K8(-/-) colonocytes, suggesting a role for keratins in colonocyte energy metabolism and homeostasis. PMID: 25904331
    9. using knockout mice, we show K8+/- colon expresses ~50% less keratins compared to K8 wild type. In experimental colitis, K8+/- mice showed higher disease sensitivity and delayed recovery compared to K8+/+ littermates. PMID: 26000979
    10. In several liver stress models epiplakin and K8 genes displayed identical expression patterns and transgenic K8 overexpression resulted in elevated hepatic epiplakin levels. PMID: 25617501
    11. Directed expression of a chimeric type II keratin partially rescues keratin 5-null mice. PMID: 24867950
    12. Our data demonstrate that HF diet triggers aggregate formation and development of liver injury in susceptible individuals through misfolding and crosslinking of excess K8. PMID: 24519272
    13. K8 inactivation leads to reduced fasting blood glucose levels and resistance to hyperglycaemia after glucose challenge. PMID: 24144696
    14. Keratin 8 is required for the maintenance of architectural structure in thymus epithelium. PMID: 24086449
    15. A conserved rod domain phosphotyrosine that is targeted by the phosphatase PTP1B promotes keratin 8 protein insolubility and filament organization. PMID: 24003221
    16. K8 acetylation at Lys-207, a highly conserved residue among type II keratins, is up-regulated upon hyperglycemia and down-regulated by SIRT2. PMID: 23358244
    17. Data indicate that cyclin D1 is expressed exclusively in luminal keratin 8 immunoreactive tumor cells and is dependent on the adipose secreted hormone leptin. PMID: 22692856
    18. Mice lacking the prostate epithelial AR have increased apoptosis in epithelial CK8-positive luminal cells and increased proliferation in epithelial CK5-positive basal cells. PMID: 21754978
    19. Data show that lack of K8 confers resistance to colonocyte apoptosis in a microflora-dependent manner. PMID: 21220329
    20. The expression of K8 is positively correlated with the liver injury induced by CCl(4). PMID: 20175234
    21. Krt8 may be a useful immunohistochemical marker for detecting hepatocellular proliferative lesions in mice. PMID: 20035116
    22. function of K8 as a plasminogen receptor PMID: 11689350
    23. potentially toxic bile acids induce hepatocytic CK 8 and CK 18 expression and phosphorylation PMID: 11839569
    24. K8 cytokeratin has a relevant role during the differentiation and tridimensional organization of the sensory and the supporting cells of the auditory receptor PMID: 12168793
    25. distribution in cell clusters in mouse embryonic tongue as a marker for a prepattern for taste bud development PMID: 12541313
    26. The role that UBB(+1) plays in cytokeratin aggresome formation in Mallory Bodies in vitro was studied. PMID: 12710947
    27. lethality of Keratin 8-/- embryos is due to a TNF-sensitive failure of trophoblast giant cell barrier function PMID: 12771125
    28. Results suggest that keratin 8 may play a role in regulating electrolyte transport, likely by targeting ion transporters to their cellular compartments. PMID: 15007064
    29. K8 overexpression is sufficient to form pre-MB and primes animals to accumulate MBs upon DDC challenge, which may help explain MB formation in human liver diseases. PMID: 16365160

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  • 亚细胞定位：
    Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
  • 蛋白家族：
    Intermediate filament family
  • 组织特异性：
    Expressed in abundance in the epithelia of colon, bladder, ileum, and stomach, with lower expression observed in earskin (at protein level). Also expressed in pancreas, liver, dudenum and jejunum.
  • 数据库链接：

    KEGG: mmu:16691

    STRING: 10090.ENSMUSP00000023952

    UniGene: Mm.358618

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