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  4. Recombinant Mouse Histone acetyltransferase p300 (Ep300), partial

Recombinant Mouse Histone acetyltransferase p300 (Ep300), partial

  • 中文名称：
    小鼠Ep300重组蛋白
  • 货号：
    CSB-EP007702MO
  • 规格：
    ￥1836
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    Ep300
  • Uniprot No.：
    B2RWS6
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 来源：
    E.coli
  • 分子量：
    50.4 kDa
  • 表达区域：
    1286-1662aa
  • 氨基酸序列
    KLSAKRLPSTRLGTFLENRVNDFLRRQNHPESGEVTVRVVHASDKTVEVKPGMKARFVDSGEMAESFPYRTKALFAFEEIDGVDLCFFGMHVQEYGSDCPPPNQRRVYISYLDSVHFFRPKCLRTAVYHEILIGYLEYVKKLGYTTGHIWACPPSEGDDYIFHCHPPDQKIPKPKRLQEWYKKMLDKAVSERIVHDYKDILKQATEDRLTSAKELPYFEGDFWPNVLEESIKELEQEEEERKREENTSNESTDVTKGDSKNAKKKNNKKTSKNKSSLSRGNKKKPGMPNVSNDLSQKLYATMEKHKEVFFVIRLIACPAPNSLPPIVDPDPLIPCDLMDGRDAFLTLARDKHLEFSSLRRAQWSTMCMLVELHTQSQ
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    C-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    13-23 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

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Ep300 Proteins

靶点详情

  • 功能：
    Functions as histone acetyltransferase and regulates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Histone acetylation gives an epigenetic tag for transcriptional activation. Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. Mediates acetylation of histone H3 at 'Lys-122' (H3K122ac), a modification that localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability. Mediates acetylation of histone H3 at 'Lys-27' (H3K27ac). Also functions as acetyltransferase for non-histone targets, such as ALX1, HDAC1, PRMT1 or SIRT2. Acetylates 'Lys-131' of ALX1 and acts as its coactivator. Acetylates SIRT2 and is proposed to indirectly increase the transcriptional activity of TP53 through acetylation and subsequent attenuation of SIRT2 deacetylase function. Acetylates HDAC1 leading to its inactivation and modulation of transcription. Acetylates 'Lys-247' of EGR2. Acts as a TFAP2A-mediated transcriptional coactivator in presence of CITED2. Plays a role as a coactivator of NEUROD1-dependent transcription of the secretin and p21 genes and controls terminal differentiation of cells in the intestinal epithelium. Promotes cardiac myocyte enlargement. Can also mediate transcriptional repression. Acetylates FOXO1 and enhances its transcriptional activity. Acetylates BCL6 wich disrupts its ability to recruit histone deacetylases and hinders its transcriptional repressor activity. Participates in CLOCK or NPAS2-regulated rhythmic gene transcription; exhibits a circadian association with CLOCK or NPAS2, correlating with increase in PER1/2 mRNA and histone H3 acetylation on the PER1/2 promoter. Acetylates MTA1 at 'Lys-626' which is essential for its transcriptional coactivator activity. Acetylates XBP1 isoform 2; acetylation increases protein stability of XBP1 isoform 2 and enhances its transcriptional activity. Acetylates PCNA; acetylation promotes removal of chromatin-bound PCNA and its degradation during nucleotide excision repair (NER). Acetylates MEF2D. Acetylates and stabilizes ZBTB7B protein by antagonizing ubiquitin conjugation and degradation, this mechanism may be involved in CD4/CD8 lineage differentiation. Acetylates GABPB1, impairing GABPB1 heterotetramerization and activity. In addition to protein acetyltransferase, can use different acyl-CoA substrates, such as (2E)-butenoyl-CoA (crotonyl-CoA), butanoyl-CoA (butyryl-CoA), 2-hydroxyisobutanoyl-CoA (2-hydroxyisobutyryl-CoA), lactoyl-CoA or propanoyl-CoA (propionyl-CoA), and is able to mediate protein crotonylation, butyrylation, 2-hydroxyisobutyrylation, lactylation or propionylation, respectively. Acts as a histone crotonyltransferase; crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. Histone crotonyltransferase activity is dependent on the concentration of (2E)-butenoyl-CoA (crotonyl-CoA) substrate and such activity is weak when (2E)-butenoyl-CoA (crotonyl-CoA) concentration is low. Also acts as a histone butyryltransferase; butyrylation marks active promoters. Catalyzes histone lactylation in macrophages by using lactoyl-CoA directly derived from endogenous or exogenous lactate, leading to stimulates gene transcription. Acts as a protein-lysine 2-hydroxyisobutyryltransferase; regulates glycolysis by mediating 2-hydroxyisobutyrylation of glycolytic enzymes. Functions as a transcriptional coactivator for SMAD4 in the TGF-beta signaling pathway. Acetylates PCK1 and promotes PCK1 anaplerotic activity. Acetylates RXRA and RXRG.
  • 基因功能参考文献：
    1. Study in mouse model found that liver stiffness activates hepatic stellate cells differentiation into myofibroblasts, which required nuclear accumulation of p300. PMID: 29454793
    2. Data (including data from studies in knockout and transgenic mice) suggest that Ep300 and Crebbp are limiting cofactors for pancreatic islet development (including gene expression regulation and cell proliferation), and hence for postnatal glucose homeostasis, with some functional redundancy. (Ep300 = E1A binding protein p300; Crebbp = CREB binding protein) PMID: 29217654
    3. Here the authors report a lipopolysaccharide-induced NFkappaB enhanceosome in which TonEBP is required for the recruitment of p300. Increased expression of TonEBP enhances the NFkappaB activity and reduced TonEBP expression lowers it. PMID: 27118681
    4. Data show that LPS induces endoplasmic reticulum (ER) stress and P300 activity via the XBP1/IRE1 pathway. PMID: 28743992
    5. Loss of p300 expression is associated with leukemogenesis. PMID: 27881875
    6. UTX-MLL4-p300 transcriptional regulatory network establishing an "active enhancer landscape" and defines a detailed mechanism for the joint deposition of H3K4me1 and H3K27ac. PMID: 28732206
    7. Acetylation-dependent control of global poly(A) RNA degradation by CBP/p300 and HDAC1-HDAC2 has been described. PMID: 27635759
    8. Data, including data from studies in cells from knockout mice, suggest that Prmt1 activity was necessary for c-Myc binding to acetyltransferase p300 in myeloid cells; Prmt1 inhibition decreases p300 recruitment to c-Myc target promoters and increased Hdac1 recruitment. [Prmt1, protein arginine N-methyltransferase 1; c-Myc = Proto-Oncogene Proteins c-myc; Hdac1 = histone deacetylase 1] PMID: 28652407
    9. In line with the acetyltransferase activity of p300, H3K27 acetylation was reduced after HDACi and resulted in the formation of heterochromatin in the PTGES1 gene. In conclusion, HDAC activity maintains PTGES1 expression by recruiting p300 to its gene PMID: 27913583
    10. 2-O, 3-O desulfated heparin inhibited HMGB1 release, at least in part, by direct molecular inhibition of p300 HAT activity. PMID: 27585400
    11. work shows that helenalin acetate inhibits C/EBPbeta by binding to the N-terminal part of C/EBPbeta, thereby disrupting the cooperation of C/EBPbeta with the co-activator p300. PMID: 27803164
    12. Findings indicate that E1a-binding protein (p300) is not required for the normal development or functioning of skeletal muscle. PMID: 26712218
    13. Hdac3 cooperates with p300 to prime and maintain oligodendrocyte identity PMID: 26859354
    14. p300 histone acetyltransferase activity is critical for Wnt-dependent palate mesenchymal cell proliferation and migration, both processes that play a significant role in morphogenesis of the palate. PMID: 26921506
    15. Data indicate that glucose-induced endothelial to mesenchymal transition in vivo and in vitro in the hearts of diabetic mice is possibly mediated by miR-200b and p300. PMID: 26718496
    16. This study demonstrated that p300 increase in skeletal muscle in muscle atrophy. PMID: 26372908
    17. This study demonstrates that p300 mediates histone acetylation of PS1 and BACE1 in a cellular model of Alzheimer's disease. PMID: 25051175
    18. p300 regulates a number of critical signaling pathways that control liver functions. PMID: 26100016
    19. p300 conditional knockout in Treg cells leads to minimal changes in Treg functions. PMID: 24835996
    20. on Bcl6, p300 functioned as a repressor and inhibited in conjunction with STAT5 or BCL6. PMID: 25088465
    21. These results suggest that the aristolochic acid dose dependently contributed to the development of nephropathy, and HDAC1 and p300 participate in the modulation of TGF-beta/Smad pathway-mediated renal interstitial fibrosis. PMID: 24796935
    22. ATF3 interacts with PPARgamma and represses PPARgamma-mediated transactivation through suppression of p300-stimulated coactivation in 3T3-L1 cells PMID: 25446101
    23. The formation of p300/CBP-mediated looping chromatin structures may recruit distal enhancers to create a concentration of factors for the transcription activation of genes that are involved in self-renewal and pluripotency. PMID: 24648406
    24. we demonstrated that inhibition of the acetylase function of p300 reduces both cell count and invasion in LM3 murine breast cancer cell line , and decreases tumor progression in the animal model PMID: 25240203
    25. This study revealed the occurrence of a tightly regulated Cbp/p300-dependent gene expression programme that drives a specific metabolic state both in progenitor spermatogenic cells and in late transcriptionally active spermatids. PMID: 24522976
    26. glucose-induced EndMT in vitro and in vivo is possibly mediated through TGFbeta and regulated by miR-200b and p300. PMID: 25335984
    27. Results show that p300 forms a complex network along with CBP and other transcription factors and function within this complex as master regulators of Foxp3+ Treg generation and cell survival. PMID: 25154413
    28. these results suggest that the KIX domains of CBP, and especially p300, are principal mediators of c-Myb-dependent gene activation and repression that is required for definitive hematopoiesis. PMID: 24340053
    29. a mutant mouse model demonstrates how c-Myb with p300 is required for oncogene-induced acute myeloid leukemia PMID: 24596419
    30. These results suggest that p300 and CBP play essential roles in maintaining photoreceptor-specific structure, function and gene expression. PMID: 23922782
    31. depletion of hepatic p300 reduces glycogen synthesis, decreases hepatic glycogen storage, and leads to relative hypoglycemia. PMID: 23770612
    32. Two independent regions of simian virus 40 T antigen increase CEBP/p300 levels and alter patterns of cellular histone acetylation. PMID: 24089570
    33. p300 is important for Foxp3(+) T(reg) cell function and homeostasis in vivo and in vitro. PMID: 23955711
    34. The p300 regulates GI differentiation primarily through Myb, rather than CREB. PMID: 23618903
    35. the role of Mdm2's RING domain in the control of p53 PMID: 22666487
    36. Studies demonstrate for the first time that PDGF-BB-induced TRAIL transcriptional activity requires the cooperation of Sp1, ac-H3 and p300, mediating increased expression of TRAIL which is important for VSMC proliferation and migration. PMID: 22415975
    37. p300 and PCAF cooperate in the control of microRNA 200c/141 transcription and epithelial characteristics PMID: 22384255
    38. Curcumin enhances the efficacy of chemotherapy by tailoring p65NFkappaB-p300 cross-talk in favor of p53-p300 in breast cancer PMID: 22013068
    39. mice in which the CBP CH1 domain structure is disrupted by deleting residues 342-393 are lean and insulin sensitized, as are p300 mutants PMID: 21803292
    40. Data support the acetyltransferase activities of p300/CBP in regulating FOXO signaling in skeletal muscle and suggests that acetylation may be an important mechanism to differentially regulate the FOXO homologues PMID: 21389279
    41. transcriptional coactivator p300 may be partially degraded during SMC differentiation, leaving an activated subpopulation with increased HAT activity and SMC differentiation-gene specificity PMID: 21179216
    42. p300 acetylates Pax5 and strongly enhances Pax5-mediated transcriptional activity PMID: 21357426
    43. Spatial conformation of chromatin in the osteopontin promoter leads to recruitment of coactivator p300, formation of a DNA looping complex with NF-kappaB and AP-1, and induction of osteopontin expression. PMID: 21257959
    44. These findings indicate possible mechanisms by which CBP/p300 tissue-specifically acts cooperatively with pCAF and HDAC3 either as a co-activator or co-repressor, respectively, for CLOCK/BMAL1. PMID: 19922678
    45. impairment of Lck-mediated CD4 coreceptor signaling by Nef is an important in vivo mechanism of HIV-1 pathogenesis PMID: 20810990
    46. Both TAZ domains of CREB-binding proteins CBP and p300 in the presence of stoichiometric concentrations of zinc adopt a well-defined structure in solution in the absence of binding partners. PMID: 15641773
    47. contribution of p300 to Rubinstein-Taybi syndrome (RSTS); analysis demonstrates p300(+/-) mice exhibit phenotypes reminiscent of RSTS patients but indicate activity of p300 in cognition likely less relevant or more susceptible to compensation than CBP PMID: 19822209
    48. KIX or CH1 domain deletion caused profound hematopoiesis defects. Losing other domains had only lineage-restricted effects. Mutation of the histone acetyltransferase domain had few effects on hematopoiesis & increased progenitor/stem-cell proliferation. PMID: 19822904
    49. conserved domains in two highly related coactivators (p300 and CBP) have contrasting roles in haematopoiesis PMID: 12384703
    50. has a distinct role in hamatopoietic stem cell self-renewal PMID: 12397173

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 数据库链接：

    KEGG: mmu:328572

    STRING: 10090.ENSMUSP00000066789

    UniGene: Mm.258397

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