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  4. Recombinant Mouse Glutamate receptor-interacting protein 1 (Grip1), partial

Recombinant Mouse Glutamate receptor-interacting protein 1 (Grip1), partial

  • 中文名称：
    小鼠Grip1重组蛋白
  • 货号：
    CSB-YP846086MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Grip1重组蛋白
  • 货号：
    CSB-EP846086MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Grip1重组蛋白
  • 货号：
    CSB-EP846086MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Grip1重组蛋白
  • 货号：
    CSB-BP846086MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Grip1重组蛋白
  • 货号：
    CSB-MP846086MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Grip1
  • Uniprot No.：
    Q925T6
  • 别名：
    Grip1Glutamate receptor-interacting protein 1; GRIP-1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

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问答及客户评论

■ 常见问题解答
Q:

Please kindly provide the amino acid sequence of the following three proteins (there should be only one)
Recombinant Mouse Glutamate receptor-interacting protein 1(Grip1) ,partial, Mammalian cell CSB-MP846086MO
Recombinant Mouse Glutamate receptor-interacting protein 1(Grip1) ,partial, Baculovirus CSB-BP846086MO
Recombinant Mouse Glutamate receptor-interacting protein 1(Grip1) ,partial, Escherichia Coli CSB-EP846086MO
CSB-CF723331MO
CSB-CF010739MO
CSB-CF727053MO
1.How pure can these proteins be? Is there more than 85%?
2.Can you provide some examples of successful expression and activity of transmembrane proteins?
3.When recombinant protein quality control will run SDS -page, will run HPLC?

A:
Thanks for your inquiry，
1.Please kindly provide the amino acid sequence of the following three proteins (there should be only one).
Recombinant Mouse Glutamate receptor-interacting protein 1(Grip1) ,partial, Mammalian cellCSB-MP846086MO
Recombinant Mouse Glutamate receptor-interacting protein 1(Grip1) ,partial, BaculovirusCSB-BP846086MO
Recombinant Mouse Glutamate receptor-interacting protein 1(Grip1) ,partial, Escherichia ColiCSB-EP846086MO
In addition, the length of this protein is 1127, so it can be recommended to customize the risk-free service and charge the cost of failure
Tag information:EP BP MP:N-terminal 10xHis-tagged and C-terminal Myc-tagged；YP:N-terminal 6xHis-tagged
Expression sequence:1-1127aa,Full length
Sequence:

MIAVSFKCRCQILRRLTKDESPYTKSASQTKPPDGALAVRRQSIPEEFKGSTVVELMKKEGTTLGLTVSGGIDKDGKPRVSNLRQGGIAARSDQLDVGDYIKAVNGINLAKFRHDEIISLLKNVGERVVLEVEYELPPVSVQGSSVMFRTVEVTLHKEGNTFGFVIRGGAHDDRNKSRPVVITCVRPGGPADREGTIKPGDRLLSVDGIRLLGTTHAEAMSILKQCGQEATLLIEYDVSVMDSVATASGPLLVEVAKTPGASLGVALTTSVCCNKQVIVIDKIKSASIADRCGALHVGDHILSIDGTSMEYCTLAEATQFLANTTDQVKLEILPHHQTRLALKGPDHVKIQRSDRQHPWDAWASNQCGVHTNHHHNTYHPDHCRVPALTFPKALPPNSPPAMVPSSSPTSMSAYSLSSLNMGTLPRSLYSTSPRGTMMRRRLKKKDFKSSLSLASSTVGLAGQVVHTETTEVVLTADPVTGFGIQLQGSVFATETLSSPPLISYIEADSPAERCGVLQIGDRVMAINGIPTEDSTFEEANQLLRDSSITSKVTLEIEFDVAESVIPSSGTFHVKLPKKHSVELGITISSPSSRKPGDPLVISDIKKGSVAHRTGTLELGDKLLAIDNIRLDNCSMEDAVQILQQCEDLVKLKIRKDEDNSDEQESSGAIIYTVELKRYGGPLGITISGTEEPFDPIIISSLTKGGLAERTGAIHIGDRILAINSSSLKGKPLSEAIHLLQMAGETVTLKIKKQTDAQSASSPKKFPIPGHSGDLGDGEEDPSPIQKPGKLSDAYPSTVPSVDSAVDSWDGSGIDASYGSQGSTFQTSGYNYNTYDWRSPKQRTSLSPVPKPRSQTYPDVGLSNEDWDRSTASGFVGASDSADAEQEENFWSQALEDLETCGQSGILRELEEKADRRVSLRNMTLLATIMSGSTMSLNHEAPMARSQLGRQASFQERSSSRPHYSQTTRSNTLPSDVGRKSVTLRKMKQEIKEIMSPTPVELHKVTLYKDSGMEDFGFSVADGLLEKGVYVKNIRPAGPGDVGGLKPYDRLLQVNHVRTRDFDCCLVVPLIAESGNKLDLVISRNPLASQKSIEQPALPSDWSEQNSAFFQQPSHGGNLETREPTNTL


2. CSB-CF723331MO
CSB-CF010739MO
CSB-CF727053MO
As they are all futures, our current quality control standard purity is more than 85%. 3.When recombinant protein quality control, we will run the SDS -page, and some will do the FPLC(akta-sec). At present, there is no HPLC instrument
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靶点详情

  • 功能：
    May play a role as a localized scaffold for the assembly of a multiprotein signaling complex and as mediator of the trafficking of its binding partners at specific subcellular location in neurons. Through complex formation with NSG1, GRIA2 and STX12 controls the intracellular fate of AMPAR and the endosomal sorting of the GRIA2 subunit toward recycling and membrane targeting.
  • 基因功能参考文献：
    1. GRIP1 is phosphorylated at an N-terminal serine cluster by cyclin-dependent kinase-9 (CDK9), which is recruited into GC-induced GR:GRIP1:CDK9 hetero-complexes, producing distinct GRE-specific GRIP1 phospho-isoforms. PMID: 29170386
    2. GRIP1 is a critical regulator of immunometabolism, which engages distinct transcriptional mechanisms to coordinate the balance between macrophage populations and ultimately promote metabolic homeostasis. PMID: 27464507
    3. Data suggest that molecules in the erythropoietin-producing hepatocellular receptor B family (EPHB) / ephrinB (EFNB) signalling pathways, specifically ephrin B3 and GRIP1, are involved blood pressure regulation. PMID: 27941904
    4. GRIP1 binds to apoER2 and EphrinB2 to induce activity-dependent AMPA receptor insertion at the synapse. PMID: 28978486
    5. Study performed social behavioral testing on neuron-specific Grip1/2-double knockout (DKO) and wild type (WT) mice and determined the expression profile of key signaling proteins in AMPAR, mGluR, mTOR, and GABA pathways in brain. Data Grip1/2-mediated AMPA signaling in regulating social behaviors and disturbance of glutamate- and GABA-signaling in specialized brain regions in autism-related social behavioral deficits. PMID: 28063882
    6. subcellular redistribution of GRIP1 and a change in the binding of GRIP1 to GluA2 during synaptic scaling, was observed. PMID: 26216979
    7. This study demonistrated that deletion of accumbal GRIP1 increases vulnerability to cue-induced cocaine relapse without altering the reinforcing properties of the drug or the response to natural rewards PMID: 24126453
    8. a regulatory role for GRIP1 during microtubule-based transport and suggest a crucial function for 14-3-3 proteins in controlling kinesin-1 motor attachment during neuronal development PMID: 24576423
    9. Data indicate that glucocorticoid receptor (GR):GRIP1 use distinct mechanisms to repress inflammatory genes at different stages of the transcription cycle. PMID: 23950223
    10. GRIP1 and 2 regulate activity-dependent AMPA receptor recycling via exocyst complex interactions. PMID: 20956289
    11. GRIP1 scaffolding protein is required for the formation and integrity of the dermo-epidermal junction and reveal the importance of PDZ domains in the organization of supramolecular structures essential for mammalian embryonic development. PMID: 11983858
    12. Glutamate-receptor-interacting protein GRIP1 directly steers kinesin to dendrites. PMID: 11986669
    13. morphology of octopus cell (OC) somata in the cochlear nucleus B6 and B6Cast mice, and the immunocytochemistry of GluR1 (glutamate receptor subunit 1) and GRIP-C (glutamate receptor interacting protein-C terminus). PMID: 12204345
    14. investigated whether there is a link between growth inhibitory effect and transcriptional activation of PPARgamma in several gastrointestinal tumour cell lines [GRIP1tau] PMID: 15507123
    15. These results indicate that the GRIP family comprises an essential molecular component for cerebellar LTD. PMID: 18509036
    16. An isoform of Grip1 interacts with the N-terminus of Dlx2. PMID: 11675124

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  • 亚细胞定位：
    [Isoform 2]: Membrane; Lipid-anchor.; Cytoplasmic vesicle. Perikaryon. Cell projection, dendrite. Cytoplasm. Endomembrane system; Peripheral membrane protein. Cell junction, synapse, postsynaptic cell membrane. Cell junction, synapse, postsynaptic density. Endoplasmic reticulum membrane; Peripheral membrane protein.
  • 组织特异性：
    Expressed in brain. Isoform 2 is the major isoform in brain. Expressed in oligodendrocyte lineage cells.
  • 数据库链接：

    KEGG: mmu:74053

    STRING: 10090.ENSMUSP00000123234

    UniGene: Mm.196692

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