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  4. Recombinant Mouse Eukaryotic translation initiation factor 4E (Eif4e)

Recombinant Mouse Eukaryotic translation initiation factor 4E (Eif4e)

  • 中文名称：
    小鼠Eif4e重组蛋白
  • 货号：
    CSB-YP007556MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Eif4e重组蛋白
  • 货号：
    CSB-EP007556MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Eif4e重组蛋白
  • 货号：
    CSB-EP007556MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Eif4e重组蛋白
  • 货号：
    CSB-BP007556MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Eif4e重组蛋白
  • 货号：
    CSB-MP007556MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Eif4e
  • Uniprot No.：
    P63073
  • 别名：
    Eif4e; Eukaryotic translation initiation factor 4E; eIF-4E; eIF4E; mRNA cap-binding protein; eIF-4F 25 kDa subunit
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-217
  • 氨基酸序列
    ATVEPETTP TTNPPPAEEE KTESNQEVAN PEHYIKHPLQ NRWALWFFKN DKSKTWQANL RLISKFDTVE DFWALYNHIQ LSSNLMPGCD YSLFKDGIEP MWEDEKNKRG GRWLITLNKQ QRRSDLDRFW LETLLCLIGE SFDDYSDDVC GAVVNVRAKG DKIAIWTTEC ENRDAVTHIG RVYKERLGLP PKIVIGYQSH ADTATKSGST TKNRFVV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. In addition to its role in translation initiation, also acts as a regulator of translation and stability in the cytoplasm. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression: in the complex, EIF4E mediates the binding to the mRNA cap. Component of a multiprotein complex that sequesters and represses translation of proneurogenic factors during neurogenesis. In P-bodies, component of a complex that mediates the storage of translationally inactive mRNAs in the cytoplasm and prevents their degradation. May play an important role in spermatogenesis through translational regulation of stage-specific mRNAs during germ cell development.
  • 基因功能参考文献：
    1. eIF4E phosphorylation modulates depression-like behavior through regulation of inflammatory responses. PMID: 29941989
    2. eIF4E availability and phosphorylation promote metastasis in mouse models of breast cancer by selectively augmenting the translation of mRNAs involved in invasion and metastasis. PMID: 29463754
    3. Lack of Def6 results in deregulation of Bcl6 protein synthesis in T cells as a result of enhanced activation of the mTORC1-4E-BP-eIF4E axis. PMID: 28811467
    4. Mitogen-activated protein kinase interacting protein kinases (Mnks) control translation by phosphorylation of eIF4E, whereas the mTOR kinase phosphorylates/de-activates the eIF4E inhibitor, 4E-BP1, to release translational repression. PMID: 27094611
    5. PRMT1 inhibition prevents gastric cancer progression by downregulating eIF4E and targeting type II PRMT5. PMID: 28987382
    6. Our work demonstrates that a single phosphorylation site on the 5' cap-binding protein eIF4E is a critical mechanism for changes in nociceptor excitability that drive the development of chronic pain. PMID: 28674170
    7. data suggest a physiological role for MNK1a-Ser(353) phosphorylation in regulation of the MNK1a kinase, which correlates with increased eIF4E phosphorylation in vitro and in vivo. PMID: 27413184
    8. Results of our study suggest that the eIF4E/Fmr1 double mutant mouse may be a reliable model to study cognitive dysfunction in the context of autism spectrum disorder. PMID: 26306459
    9. Unphosphorylated HSP27 associates with eIF4E in osteoblasts and suppresses the translation initiation process. PMID: 26151374
    10. Rotenone induction of hydrogen peroxide inhibits mTOR-mediated S6K1 and 4E-BP1/eIF4E pathways, resulting in caspase-dependent and -independent apoptosis in neuronal cells. PMID: 25304210
    11. Findings indicate eIF4E is maintained at levels in excess for normal development that are hijacked by cancer cells to drive a translational program supporting tumorigenesis. PMID: 26095252
    12. a light- and circadian clock-regulated MAPK/MNK pathway led to phosphorylation of the cap-binding protein eIF4E PMID: 25915475
    13. mice in which eukaryotic translation initiation factor 4E (eIF4E) cannot be phosphorylated are resistant to lung metastases in a mammary tumor model, and cells isolated from these mice exhibit impaired invasion. PMID: 24909168
    14. Data suggest that MAP kinase-interacting kinases (Mnk1, Mnk2) regulate cell migration/wound healing, expression of vimentin, stability of vimentin protein, and binding of eIF4E and Cyfip1 (cytoplasmic FMR1 interacting protein 1). PMID: 25588502
    15. results show that the activation of p38 and Mnk during MNV1 infection is important for MNV1 replication. Furthermore, phosphorylated eIF4E relocates to the polysomes, and this contributes to changes in PMID: 25561727
    16. Maintaining eIF4E levels below its proneoplastic threshold is an important anticancer defense in normal cells, with important implications for understanding pregnancy-associated breast cancer. PMID: 25524901
    17. The mTORC1 effectors, RPS6 and eIF4E, play distinct roles and are both necessary for AKT/Ras hepatocarcinogenesis. PMID: 25145583
    18. repeated activation of markers of translation initiation eIF4E and 4EBP1 in hippocampus during the circadian cycle might be critical for memory persistence PMID: 24895127
    19. eIF4E phosphorylation is enhanced by Rapamycin, which activates Mnk2a PMID: 23831578
    20. eIF4F suppression in breast cancer affects maintenance and progression. PMID: 22484424
    21. EIF4E is defined as a Myc client responsible for augmenting prosurvival and proliferative capacity through effectors, such as Mcl-1 and Cyclin D1. PMID: 22573234
    22. translational control by eIF4E regulates the synthesis of neuroligins, maintaining the excitation-to-inhibition balance, and its dysregulation engenders autism spectrum disorder-like phenotypes PMID: 23172145
    23. increasing the levels eIF4E in mice results in exaggerated cap-dependent translation and aberrant behaviours reminiscent of autism, including repetitive and perseverative behaviours and social interaction deficits PMID: 23263185
    24. The oncogene eIF4E reprograms the nuclear pore complex to promote mRNA export and oncogenic transformation. PMID: 22902403
    25. Disruption of the eukaryotic translation initiation factor 4E - Gigyf2 complex leads to increased translation and perinatal lethality in mice. PMID: 22751931
    26. the activity of eIF4E is under homeostatic control via the regulation of the levels of its repressor protein 4E-BP1 through ubiquitination. PMID: 22578813
    27. findings reveal unique developmental functions for eIF4E and S6 kinase wherein their activity is specifically uncoupled by mitogenic Shh signaling PMID: 21339731
    28. phosphorylation of eIF4E is required for translational up-regulation of several proteins implicated in tumorigenesis PMID: 20679199
    29. SNF5 knockdown inhibits p53 translation by eIF4E and replacement of eIF4E in SNF5 knockdown cells restores p53 expression and cell survival PMID: 20473326
    30. Inducible expression of murine eIF4E increased translation of defined sets of 3T3-cell mRNAs. Many are novel targets, including those that encode large- & small-subunit ribosomal proteins, cell growth-related factors, & anti-apoptotic proteins. PMID: 17311107
    31. Biophysical studies of eIF4E cap-binding protein: recognition of mRNA 5' cap structure and synthetic fragments of eIF4G and 4E-BP1 proteins PMID: 12054859
    32. thermodynamics of eIF4E binding to a cap-analogue, 7-methylGpppG PMID: 12356315
    33. Inhibition of pancreas protein synthesis in model of acute pancreatitis likely results from inhib of translation initiation from increased eIF2alpha phosphorylation,reduced EIF2B activity and inhib of eIF4F complex formation a component of which is Eif4e PMID: 12773302
    34. Mutation of the Usf2 gene was associated with reduced abundance of the eukaryotic initiation factors eIF4E and eIF4G PMID: 12907752
    35. Results obtained with mutants of eIF4E and the phosphorylated protein clearly show that electrostatic repulsion is the main factor that reduces the binding affinity of the phosphorylated protein for capped mRNAs. PMID: 15122903
    36. Emx2 axonal localization, association with high-density particles and interaction with eIF4E suggest that Emx2 has nonnuclear functions probably related to the local control of protein translation in olfactory sensory neuron axons. PMID: 15247416
    37. phosphorylation of eukaryotic initiation factor 4E requires Mnk2 and Mnk1 PMID: 15254222
    38. Thermodynamic studies reveal the unique, large and positive heat capacity changes at constant pressure that accompany interactions between eIF4E and the mRNA 5' cap. PMID: 15491137
    39. mutation and affinity study of variant eIF4E proteins PMID: 15611299
    40. Mnk activation by oxidative stress leading to enhanced eIF4E phosphorylation may play a role in promoting stress-induced hyperproliferative diseases, such as smooth muscle cell proliferation and hypertrophy in cardiovascular disease PMID: 15683719
    41. eIF4E inhibits erythroid differentiation PMID: 16166632
    42. Our results provide direct evidence that cap-dependent translation is engaged during mGluR-LTD and demonstrate that the MEK-ERK and PI3K-mTOR signaling pathways converge to regulate eIF4E activity after induction of DHPG-LTD. PMID: 16495443
    43. kinetic analysis of eIF4E and phosphorylated eIF4E binding to cap analogs and capped oligoribonucleotides PMID: 16540463
    44. eIF4E is a criticla node in an RNA regulon impacting cell cycle progression. eIF4E coordinately promotes mRNA export of cell cycle genes. PMID: 17074885
    45. NGF regulates protein synthesis in the nervous tissue by enhancing expression and phosphorylation of eIF4E. PMID: 17684707
    46. Roles of mitogen-activated protein kinase signal-integrating kinases 1 and 2 in oxidant-mediated eIF4E phosphorylation. PMID: 17689282
    47. cancers may be more susceptible to eIF4E inhibition than normal tissues PMID: 17786246
    48. Phosphorylated eIF4E promotes tumorigenesis primarily by suppressing apoptosis and, accordingly, the anti-apoptotic protein Mcl-1 is one target of both phospho-eIF4E and MNK1 that contributes to tumor formation PMID: 18055695
    49. eIF4E coordinately regulates gene expression to potentiate Akt activation, an activity required for apoptotic rescue. PMID: 18391071
    50. Study shows that a sizable fraction (approximately 30%) of 4E-BP1 is localized to the nucleus, where it binds eIF4E. PMID: 18515545

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  • 亚细胞定位：
    Cytoplasm, P-body. Cytoplasm. Cytoplasm, Stress granule. Nucleus.
  • 蛋白家族：
    Eukaryotic initiation factor 4E family
  • 数据库链接：

    KEGG: mmu:13684

    STRING: 10090.ENSMUSP00000029803

    UniGene: Mm.3941

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