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  4. Recombinant Mouse Endonuclease G, mitochondrial (Endog)

Recombinant Mouse Endonuclease G, mitochondrial (Endog)

  • 中文名称：
    小鼠Endog重组蛋白
  • 货号：
    CSB-YP007665MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Endog重组蛋白
  • 货号：
    CSB-EP007665MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Endog重组蛋白
  • 货号：
    CSB-EP007665MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Endog重组蛋白
  • 货号：
    CSB-BP007665MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Endog重组蛋白
  • 货号：
    CSB-MP007665MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Endog
  • Uniprot No.：
    O08600
  • 别名：
    Endog; Endonuclease G; mitochondrial; Endo G; EC 3.1.30.-
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    45-294
  • 氨基酸序列
    ADLPAL PGGPAGGTGE LAKYGLPGVA QLRSRESYVL SYDPRTRGAL WVLEQLRPER LRGDGDRSAC DFREDDSVHA YHRATNADYR GSGFDRGHLA AAANHRWSQR AMDDTFYLSN VAPQVPHLNQ NAWNNLERYS RSLTRTYQNV YVCTGPLFLP RTEADGKSYV KYQVIGKNHV AVPTHFFKVL ILEAAGGQIE LRSYVMPNAP VDETIPLERF LVPIESIERA SGLLFVPNIL ARAGNLKAIT AGSK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Endonuclease that preferentially catalyzes the cleavage of double-stranded 5-hydroxymethylcytosine (5hmC)-modified DNA. The 5hmC-modified nucleotide does not increase the binding affinity, but instead increases the efficiency of cutting and specifies the site of cleavage for the modified DNAs. Shows significantly higher affinity for four-stranded Holliday junction over duplex and single-stranded DNAs. Promotes conservative recombination when the DNA is 5hmC-modified. Promotes autophagy through the suppression of mTOR by its phosphorylation-mediated interaction with YWHAG and its endonuclease activity-mediated DNA damage response. GSK3-beta mediated phosphorylation of ENDOG enhances its interaction with YWHAG, leading to the release of TSC2 and PIK3C3 from YWHAG resulting in mTOR pathway suppression and autophagy initiation. Promotes cleavage of mtDNA in response to oxidative and nitrosative stress, in turn inducing compensatory mtDNA replication.
  • 基因功能参考文献：
    1. These results support the involvement of redox signaling in the control of cardiomyocyte growth by ENDOG and suggest a pathway relating mtDNA content to the regulation of cell growth probably involving humanin, which prevents reactive oxygen radicals accumulation and hypertrophy induced by Endog deficiency. PMID: 29502044
    2. Deficiency of AKT2 in myocardium results in diminished MEF2A abundance, which induced decreased size of cardiomyocytes. We additionally confirmed that EndoG, which is also regulated by AKT2, is a suppressor of MEF2A in myocardium. PMID: 28965945
    3. increased browning of white adipose tissue elicited by the lack of EndoG was associated with a better glucose tolerance and reduced fat mass PMID: 27547848
    4. This study demonistated that Endonuclease G knockout mice reveals a new putative molecular player in the regulation of anxiety. PMID: 23603365
    5. These findings determine a role for EndoG in the generation of Switch region double-strand DNA breaks and class switch DNA recombination. PMID: 21111482
    6. EndoG is essential during early embryogenesis and plays a critical role in normal apoptosis and nuclear DNA fragmentation PMID: 14663139
    7. endonuclease G is essential for embryonic survival, but not through a mitochondrial or apoptosis function PMID: 15601850
    8. These data suggest that the early nuclear translocation of endoG occurs and could induce DNA fragmentation in the ischemic brain after cerebral ischemia. PMID: 15979239
    9. EndoG null mice are viable and develop to adulthood with no obvious abnormalities, suggesing that EndoG is not essential for early embryogenesis and apoptosis. PMID: 16239930
    10. high levels of EndoG were found in adult liver, heart, muscle & to a lesser extent, brain & kidney; EndoG had little or no expression in adult spleen & in embryonic organs at E13.5 stage PMID: 17762883
    11. The translocation of EndoG to the nucleus of neurons in the infarct implicates EndoG in ischemic neuronal degeneration after permanent MCA occlusion in mice. PMID: 19139873
    12. Mouse EndoG without the mitochondrial localization signal (amino-acid residues 1-43) was successfully overexpressed, purified and crystallized using a microbatch method under oil PMID: 19407388
    13. Endo G has a role in neuronal cell death as a result of oxidative stress. PMID: 19582370

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  • 亚细胞定位：
    Mitochondrion.
  • 蛋白家族：
    DNA/RNA non-specific endonuclease family
  • 数据库链接：

    KEGG: mmu:13804

    STRING: 10090.ENSMUSP00000015481

    UniGene: Mm.474473

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