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  4. Recombinant Mouse DNA polymerase zeta catalytic subunit (Rev3l), partial

Recombinant Mouse DNA polymerase zeta catalytic subunit (Rev3l), partial

  • 中文名称：
    小鼠Rev3l重组蛋白
  • 货号：
    CSB-YP726750MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Rev3l重组蛋白
  • 货号：
    CSB-EP726750MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Rev3l重组蛋白
  • 货号：
    CSB-EP726750MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Rev3l重组蛋白
  • 货号：
    CSB-BP726750MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Rev3l重组蛋白
  • 货号：
    CSB-MP726750MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Rev3l
  • Uniprot No.：
    Q61493
  • 别名：
    Rev3l; Polz; Sez4DNA polymerase zeta catalytic subunit; EC 2.7.7.7; Protein reversionless 3-like; REV3-like; Seizure-related protein 4
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalytic subunit of the DNA polymerase zeta complex, an error-prone polymerase specialized in translesion DNA synthesis (TLS). Lacks an intrinsic 3'-5' exonuclease activity and thus has no proofreading function.
  • 基因功能参考文献：
    1. Following challenge with AFB1, survival of mouse cells deficient in pol zeta (Rev3L(-/-)) was significantly reduced relative to Rev3L(+/-) cells or Rev3L(-/-) cells complemented through expression of the wild-type human REV3L PMID: 27849610
    2. A single aspartate mutation in the conserved catalytic site of Rev3L generates a hypomorphic phenotype in vivo and in vitro. PMID: 27481099
    3. the functions of REV3L in maintaining cell viability, embryonic viability and genomic stability are directly dependent on its polymerase activity, and cannot be ameliorated by an additional deletion of pol eta PMID: 26727495
    4. results can be unified by a model in which slowly dividing cells accumulate replication-associated DNA breaks but otherwise survive Rev3L deletion, but functional polzeta is essential for responses requiring rapid proliferation PMID: 23386725
    5. Structural basis of Rev1-mediated assembly of a quaternary vertebrate translesion polymerase complex consisting of Rev1, heterodimeric polymerase (Pol) zeta, and Pol kappa PMID: 22859295
    6. DNA polymerase zeta generates tandem mutations in immunoglobulin variable regions PMID: 22615128
    7. Pol zeta was determined to be necessary for proliferation of normal cells using conditions in which Rev3L could be conditionally inactivated by Cre recombinase. PMID: 22319213
    8. REV7 subunit of pol zeta mediated the interaction between REV3 and the REV1 C terminus. PMID: 22303021
    9. In the presence of reduced levels of Rev3, an essential component of Polzeta, tumors exhibited pronounced sensitivity to cisplatin, leading to a significant extension in overall survival of treated recipient mice. PMID: 21068376
    10. found that Rev3 is important for a post-replication repair pathway of helix-distorting [6-4]pyrimidine-pyrimidone photoproducts PMID: 19783229
    11. Involvement of mouse Rev3 in tolerance of endogenous and exogenous DNA damage PMID: 11884603
    12. An essential role for REV3 in cell survival: absence of REV3 induces p53-independent embryonic death PMID: 12051777
    13. data support a central role for DNA polymerase eta in the error-free bypass of UV photoproducts PMID: 14517346
    14. Immortalized mouse cell lines that lack a functional Rev3 gene are hypersensitive to UV irradiation and cisplatin treatment. PMID: 15177183
    15. Pol zeta ablation in B cells impairs the germinal center reaction, class switch recombination, DNA break repair, and genome stability. PMID: 19204108

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    DNA polymerase type-B family
  • 数据库链接：

    KEGG: mmu:19714

    STRING: 10090.ENSMUSP00000019986

    UniGene: Mm.439723

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