Your Good Partner in Biology Research

中文简体

CSF2｜ CTLA4｜ DLL3｜ EGFR｜ IL4｜ IL4R｜ LAG3｜ MSTN｜ NECTIN4｜ NT5E｜ SEZ6｜ TM4SF1｜ TNFSF13｜ TPO

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Mouse Cytoplasmic aconitate hydratase (Aco1), partial

Recombinant Mouse Cytoplasmic aconitate hydratase (Aco1), partial

  • 中文名称：
    Recombinant Mouse Cytoplasmic aconitate hydratase(Aco1) ,partial
  • 货号：
    CSB-YP001161MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Mouse Cytoplasmic aconitate hydratase(Aco1) ,partial
  • 货号：
    CSB-EP001161MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Mouse Cytoplasmic aconitate hydratase(Aco1) ,partial
  • 货号：
    CSB-EP001161MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Mouse Cytoplasmic aconitate hydratase(Aco1) ,partial
  • 货号：
    CSB-BP001161MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Mouse Cytoplasmic aconitate hydratase(Aco1) ,partial
  • 货号：
    CSB-MP001161MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Aco1
  • Uniprot No.：
    P28271
  • 别名：
    Aco1; Ireb1; IrebpCytoplasmic aconitate hydratase; Aconitase; EC 4.2.1.3; Citrate hydro-lyase; Iron regulatory protein 1; IRP1; Iron-responsive element-binding protein 1; IRE-BP 1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Bifunctional iron sensor that switches between 2 activities depending on iron availability. Iron deprivation, promotes its mRNA binding activity through which it regulates the expression of genes involved in iron uptake, sequestration and utilization. Binds to iron-responsive elements (IRES) in the untranslated region of target mRNAs preventing for instance the translation of ferritin and aminolevulinic acid synthase and stabilizing the transferrin receptor mRNA.; Conversely, when cellular iron levels are high, binds a 4Fe-4S cluster which precludes RNA binding activity and promotes the aconitase activity, the isomerization of citrate to isocitrate via cis-aconitate.
  • 基因功能参考文献：
    1. IL-6 likely up-regulates IRP1 and DMT1 expression and down-regulates FPN1 expression in BV2 microglial cells through JNK signaling pathways PMID: 28672025
    2. Using whole RNA sequencing of bone marrow cells in iron-overloaded mice, it was observed that Idh1 and Aco1, enzymes involved in the TCA cycle, were elevated. PMID: 27380194
    3. The Irp1-/- mice develop polycythemia and pulmonary hypertension, and suggesting that Irp1 plays an essential role in erythropoiesis and in the pulmonary and cardiovascular systems. PMID: 25771171
    4. IRP1 and IRP2 mutant mice rapidly succumb to systemic infection with Salmonella Typhimurium, a pathogenic bacterium that multiplies within macrophages, with increased bacterial burdens in liver and spleen. PMID: 26190773
    5. results uncover an unexpected protective role of IRP1 in pathological conditions associated with altered Fe-S metabolism PMID: 25651183
    6. Aco1 KD in fully differentiated 3T3-L1 adipocytes decreased lipogenic, Idh1, Adipoq, and Glut4 gene expression. PMID: 25550467
    7. impaired mitochondrial [Fe-S] cluster biogenesis in Mfrn1(gt/gt) cells results in elevated IRP1 RNA-binding that attenuates ALAS2 mRNA translation and protoporphyrin accumulation PMID: 24509859
    8. Data indicate that JTR-009, a benzimidazole, operated by preventing iron-regulatory protein-1 (IRP1) from binding to the iron-responsive element (IRE) in APP mRNA. PMID: 23935819
    9. Human ACO1 is a moonlighting protein that has both aconitase enzyme activity as well as an mRNA binding function. PMID: 8041788
    10. Aco1 is a moonlighting protein that has aconitase activity as well as mRNA binding ability. PMID: 1946430
    11. IRP1 is the principal regulator of HIF2alpha mRNA translation PMID: 23777768
    12. Irp1 deletion enhanced HIF2alpha protein expression in kidneys of Irp1(-/-) mice. PMID: 23395173
    13. These results uncover separable physiological roles of each IRP and identify IRP1 as a therapeutic target for manipulating HIF-2alpha action in hematologic, oncologic, and other disorders. PMID: 23395174
    14. The RNA-binding and aconitase forms of IRP-1 can undergo interconversion dependent on the density of cells growing in culture. PMID: 22544036
    15. aconitase functions as a licensing factor in ERK-dependent proliferation and differentiation, thereby providing a regulatory input for iron in EPO-dependent erythropoiesis. PMID: 21887333
    16. A wide network of mRNAs and proteins with iron-dependent regulation, IRP-dependent regulation, or both. PMID: 21940823
    17. Iron regulatory protein 1 outcompetes iron regulatory protein 2 in regulating cellular iron homeostasis in response to nitric oxide. PMID: 21566147
    18. Mice lacking both IRP1 in their hepatocytes suffer from mitochondrial iron deficiency and dysfunction associated with alterations of the ISC and heme biosynthetic pathways, leading to liver failure and death. PMID: 20674864
    19. Iron regulatory protein-1 (Irp1)/aconitase activities studied in macrophages grown under control conditions or subjected to iron starvation or supplementation show that Irp1 is activated by direct disassembly of the Fe-S cluster of cytoplasmic aconitase. PMID: 12044177
    20. A comparative study of the roles of iron regulatory proteins 1 and 2 in regulating iron metabolism. PMID: 14726953
    21. targeted mutagenesis; mice that are homozygous for the correctly modified IRP1 or IRP2 alleles, respectively, display a strong reduction (90%, IRP1(-/-)) or nondetectable levels (IRP2(-/-)) of the targeted proteins PMID: 15208438
    22. IRP1 is nitrated in nitric oxide-producing murine macrophages PMID: 15258160
    23. under conditions of O2*- -mediated oxidative stress, IRP1 is not essential for the maintenance of iron metabolism in mammals PMID: 15557328
    24. Cytosolic Fe-S cluster aconitase activity of iron regulatory protein-1 progressively decreases in Friedreich ataxia PMID: 15615771
    25. Removal of nitric oxide resulted in a reverse regulation of IRP1 activities accompanied by a decrease of labile iron pool PMID: 15629469
    26. IRP1 controls iron homeostasis during cell proliferation, inflammation, and in response to diseases altering cytosolic Fe-S cluster assembly or disassembly. PMID: 16424901
    27. IRP-1,-2 are indispensable for regulation of mammalian iron homeostasis at the post-implantation stage of murine embryonic development PMID: 16480904
    28. Data show that IRP1 are thus essential for intestinal function and organismal survival and coordinate the synthesis of key iron metabolism proteins in the duodenum. PMID: 18177727
    29. IRP1 and IRP2 increase neuronal vulnerability to oxidative injury. PMID: 18655771
    30. the response of Irp1 (-/-) and Irp2 (-/-) mice to acute local inflammation is largely preserved PMID: 19533074

    显示更多

    收起更多

  • 亚细胞定位：
    Cytoplasm, cytosol.
  • 蛋白家族：
    Aconitase/IPM isomerase family
  • 数据库链接：

    KEGG: mmu:11428

    STRING: 10090.ENSMUSP00000100038

    UniGene: Mm.331547

Most popular with customers

产品中心
特别关注


X
九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |