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  4. Recombinant Mouse Cryptochrome-1 (Cry1)

Recombinant Mouse Cryptochrome-1 (Cry1)

  • 中文名称：
    Recombinant Mouse Cryptochrome-1(Cry1)
  • 货号：
    CSB-YP006005MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Mouse Cryptochrome-1(Cry1)
  • 货号：
    CSB-EP006005MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Mouse Cryptochrome-1(Cry1)
  • 货号：
    CSB-EP006005MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Mouse Cryptochrome-1(Cry1)
  • 货号：
    CSB-BP006005MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Mouse Cryptochrome-1(Cry1)
  • 货号：
    CSB-MP006005MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Cry1
  • Uniprot No.：
    P97784
  • 别名：
    Cry1Cryptochrome-1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-606
  • 氨基酸序列
    MGVNAVHWFR KGLRLHDNPA LKECIQGADT IRCVYILDPW FAGSSNVGIN RWRFLLQCLE DLDANLRKLN SRLFVIRGQP ADVFPRLFKE WNITKLSIEY DSEPFGKERD AAIKKLATEA GVEVIVRISH TLYDLDKIIE LNGGQPPLTY KRFQTLVSKM EPLEMPADTI TSDVIGKCMT PLSDDHDEKY GVPSLEELGF DTDGLSSAVW PGGETEALTR LERHLERKAW VANFERPRMN ANSLLASPTG LSPYLRFGCL SCRLFYFKLT DLYKKVKKNS SPPLSLYGQL LWREFFYTAA TNNPRFDKME GNPICVQIPW DKNPEALAKW AEGRTGFPWI DAIMTQLRQE GWIHHLARHA VACFLTRGDL WISWEEGMKV FEELLLDADW SINAGSWMWL SCSSFFQQFF HCYCPVGFGR RTDPNGDYIR RYLPVLRGFP AKYIYDPWNA PEGIQKVAKC LIGVNYPKPM VNHAEASRLN IERMKQIYQQ LSRYRGLGLL ASVPSNSNGN GGLMGYAPGE NVPSCSSSGN GGLMGYAPGE NVPSCSGGNC SQGSGILHYA HGDSQQTHSL KQGRSSAGTG LSSGKRPSQE EDAQSVGPKV QRQSSN
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional repressor which forms a core component of the circadian clock. The circadian clock, an internal time-keeping system, regulates various physiological processes through the generation of approximately 24 hour circadian rhythms in gene expression, which are translated into rhythms in metabolism and behavior. It is derived from the Latin roots 'circa' (about) and 'diem' (day) and acts as an important regulator of a wide array of physiological functions including metabolism, sleep, body temperature, blood pressure, endocrine, immune, cardiovascular, and renal function. Consists of two major components: the central clock, residing in the suprachiasmatic nucleus (SCN) of the brain, and the peripheral clocks that are present in nearly every tissue and organ system. Both the central and peripheral clocks can be reset by environmental cues, also known as Zeitgebers (German for 'timegivers'). The predominant Zeitgeber for the central clock is light, which is sensed by retina and signals directly to the SCN. The central clock entrains the peripheral clocks through neuronal and hormonal signals, body temperature and feeding-related cues, aligning all clocks with the external light/dark cycle. Circadian rhythms allow an organism to achieve temporal homeostasis with its environment at the molecular level by regulating gene expression to create a peak of protein expression once every 24 hours to control when a particular physiological process is most active with respect to the solar day. Transcription and translation of core clock components (CLOCK, NPAS2, ARNTL/BMAL1, ARNTL2/BMAL2, PER1, PER2, PER3, CRY1 and CRY2) plays a critical role in rhythm generation, whereas delays imposed by post-translational modifications (PTMs) are important for determining the period (tau) of the rhythms (tau refers to the period of a rhythm and is the length, in time, of one complete cycle). A diurnal rhythm is synchronized with the day/night cycle, while the ultradian and infradian rhythms have a period shorter and longer than 24 hours, respectively. Disruptions in the circadian rhythms contribute to the pathology of cardiovascular diseases, cancer, metabolic syndromes and aging. A transcription/translation feedback loop (TTFL) forms the core of the molecular circadian clock mechanism. Transcription factors, CLOCK or NPAS2 and ARNTL/BMAL1 or ARNTL2/BMAL2, form the positive limb of the feedback loop, act in the form of a heterodimer and activate the transcription of core clock genes and clock-controlled genes (involved in key metabolic processes), harboring E-box elements (5'-CACGTG-3') within their promoters. The core clock genes: PER1/2/3 and CRY1/2 which are transcriptional repressors form the negative limb of the feedback loop and interact with the CLOCK|NPAS2-ARNTL/BMAL1|ARNTL2/BMAL2 heterodimer inhibiting its activity and thereby negatively regulating their own expression. This heterodimer also activates nuclear receptors NR1D1/2 and RORA/B/G, which form a second feedback loop and which activate and repress ARNTL/BMAL1 transcription, respectively. CRY1 and CRY2 have redundant functions but also differential and selective contributions at least in defining the pace of the SCN circadian clock and its circadian transcriptional outputs. More potent transcriptional repressor in cerebellum and liver than CRY2, though more effective in lengthening the period of the SCN oscillator. On its side, CRY2 seems to play a critical role in tuning SCN circadian period by opposing the action of CRY1. With CRY2, is dispensable for circadian rhythm generation but necessary for the development of intercellular networks for rhythm synchrony. Capable of translocating circadian clock core proteins such as PER proteins to the nucleus. Interacts with CLOCK-ARNTL/BMAL1 independently of PER proteins and is found at CLOCK-ARNTL/BMAL1-bound sites, suggesting that CRY may act as a molecular gatekeeper to maintain CLOCK-ARNTL/BMAL1 in a poised and repressed state until the proper time for transcriptional activation. Represses the CLOCK-ARNTL/BMAL1 induced transcription of BHLHE40/DEC1, ATF4, MTA1, KLF10 and NAMPT. May repress circadian target genes expression in collaboration with HDAC1 and HDAC2 through histone deacetylation. Mediates the clock-control activation of ATR and modulates ATR-mediated DNA damage checkpoint. In liver, mediates circadian regulation of cAMP signaling and gluconeogenesis by binding to membrane-coupled G proteins and blocking glucagon-mediated increases in intracellular cAMP concentrations and CREB1 phosphorylation. Inhibits hepatic gluconeogenesis by decreasing nuclear FOXO1 levels that downregulates gluconeogenic gene expression. Besides its role in the maintenance of the circadian clock, is also involved in the regulation of other processes. Represses glucocorticoid receptor NR3C1/GR-induced transcriptional activity by binding to glucocorticoid response elements (GREs). Plays a key role in glucose and lipid metabolism modulation, in part, through the transcriptional regulation of genes involved in these pathways, such as LEP or ACSL4. Represses PPARD and its target genes in the skeletal muscle and limits exercise capacity. Plays an essential role in the generation of circadian rhythms in the retina. Represses the transcriptional activity of NR1I2.
  • 基因功能参考文献：
    1. insulin-activated SREBP1c downregulates gluconeogenesis through CRY1-mediated FOXO1 degradation. PMID: 27412556
    2. Circadian clock cryptochrome proteins Cry1 and Cry2 regulate autoimmunity. PMID: 29109286
    3. Deleting the Cry1 intronic enhancer in mice shortens the circadian locomotor period PMID: 29572261
    4. CRY1/2 seem to repress a distinct subset of PPAR delta target genes in muscle compared to the co-repressor NCOR1. In vivo, genetic disruption of Cry1 and Cry2 enhances sprint exercise performance in mice. PMID: 28683290
    5. Knockout-rescue embryonic stem cell-derived mouse reveals that CRY1 determines circadian period through both its degradation-dependent and -independent pathways. PMID: 28017587
    6. hnRNP Q binds to mCry1 mRNA via the 5'UTR. Furthermore, hnRNP Q inhibits the translation of mCry1 mRNA, leading to altered rhythmicity in the mCRY1 protein profile. PMID: 27392095
    7. In vivo knockdown of Rfk, Riboflavin (vitamin B2) kinase essential for FAD synthesis, altered the expression rhythms of CRY1, CRY2, and PER1 PMID: 28402850
    8. The present study identified USP7 and TDP-43 as the regulators of CRY1 and CRY2, underscoring the significance of the stability control process of CRY proteins for period determination in the mammalian circadian clockwork. PMID: 27123980
    9. Cryptochrome 1 in retinal cone photoreceptors suggests a novel functional role in mammals. PMID: 26898837
    10. polyamines control the circadian period in cultured cells and animals by regulating the interaction between the core clock repressors PER2 and CRY1 PMID: 26456331
    11. Overexpression of CRY1 protects against the development of atherosclerosis via the TLR/NFkappaB pathway PMID: 26218278
    12. uncovered a novel biological role for CUL4A-DDB1-CDT2 E3 ligase that regulates molecular circadian behaviors via promoting ubiquitination-dependent degradation of CRY1 PMID: 26431207
    13. Data suggest that cryptochromes (Cry1 and Cry2) mediate periodic binding of Ck2b (casein kinase 2beta) to Bmal1 (aryl hydrocarbon receptor nuclear translocator-like protein) and thus inhibit Bmal1-Ser90 phosphorylation by Ck2a (casein kinase 2alpha). PMID: 26562092
    14. demonstrate that genotoxic stress stimulates Cry1 phosphorylation and its deubiquitination by Herpes virus associated ubiquitin-specific protease (Hausp, a.k.a Usp7), stabilizing Cry1 and shifting circadian clock time PMID: 25756610
    15. Cry1/Cry2-deficient mice had significantly lower N6- methyladenosine methylation of RNA and lost the circadian rhythm of N6-methyladenosine levels in RNA. PMID: 26239657
    16. these results suggested that the overexpression of CRY1 inhibited sleep deprivation-induced vascular inflammation that might be associated with NF-kappaB and cAMP/PKA pathways. PMID: 25435215
    17. Report compression of daily activity time in Cry1 mutant mice. PMID: 24527953
    18. the daily dissonance between peripheral clocks and the environment did not affect the lifespan of Cry1(-/-) or Cry2(-/-) mice. PMID: 24386234
    19. Study determined the crystal structure of a complex comprising the photolyase homology region of mouse CRY1 (mCRY1) and a C-terminal mouse PER2 (mPER2) fragment and provides evidence that mCRY1/mPER2 complex formation is modulated by an interplay of zinc binding and mCRY1 disulfide bond formation, which may be influenced by the redox state of the cell. PMID: 24855952
    20. The results demonstrate that Cry1 is a time-gated modulator of the ATR-mediated DNA damage checkpoint response. PMID: 24489120
    21. The results suggest that the 3'UTR of Cry1 is important for its rhythmic translation, and AUF1 bound to the 3'UTR facilitates interaction with the 5' end of mRNA by interacting with translation initiation factors and recruiting the 40S ribosomal subunit to initiate translation of Cry1 mRNA. PMID: 24423872
    22. data suggest a new role for the C-terminal tail of CRY1 in which phosphorylation rhythmically regulates CRY1 stability and contributes to the proper circadian period length PMID: 24158435
    23. miR-185 plays a role in the fine tuned regulation of CRY1 protein expression by controlling the rhythmicity of Cry1 mRNA translation. PMID: 23699394
    24. Cry1 knockout mice ar susceptible to diet-induced obesity. PMID: 23531614
    25. Study reports crystal structures of full-length dCRY, a dCRY loop deletion construct, and the photolyase homology region of mouse CRY1 (mCRY1). PMID: 23746849
    26. Parallel PER-dependent and PER-independent pathways direct circadian expression of mCRY1, the principal negative feedback regulator in the suprachiasmatic nucleus. PMID: 23690615
    27. CRY1 knockout mice are behaviorally arrhythmic, but their suprachiasmatic nucleus expresses short period rhythms. PMID: 23616524
    28. In the suprachiasmatic nucleus, uncoupled Cry1-/- cells are nearly arrhythmic, whereas Cry2-/- cells are strongly rhythmic with long period. PMID: 23223370
    29. type II protein arginine methyltransferase 5 has a role in the regulation of Circadian Per1 and CRY1 genes PMID: 23133559
    30. FBXL21 plays a dual role: protecting CRY 1/2 proteins (CRY) from FBXL3 degradation in the nucleus and promoting CRY degradation within the cytoplasm. PMID: 23452855
    31. Study shows that FBXL21 ubiquitinates CRY1 and CRY2 and that, surprisingly, FBXL21 stabilizes CRYs. FBXL21 localizes predominantly in the cytosol, whereas FBXL3 is present in the nucleus. PMID: 23452856
    32. USP2a potentially mediates circadian disruption by suppressing the CRY1 degradation during inflammation. PMID: 22669941
    33. Cryptochrome1 (Cry1) mRNA and protein are markedly decreased in the experimental collagen antibody-induced animals. PMID: 21113809
    34. CRY proteins influence cell cycle progression in a cell-autonomous, but circadian clock-independent manner and that the accelerated cell cycle progression of Cry-deficient cells is caused by global dysregulation of Bmal1-dependent gene expression. PMID: 22033214
    35. 24-h mRNA rhythms of the following genes were suppressed in db/db mice compared with control mice: the clock genes period homolog 1/2 (Per1/2) and cryptochrome 1/2 (Cry1/2). PMID: 22140039
    36. cryptochromes 1 and 2 associate with a glucocorticoid response element in the phosphoenolpyruvate carboxykinase 1 promoter in a hormone-dependent manner PMID: 22170608
    37. Data show that Potorous CPD-photolyase can restore the molecular oscillator in the liver of (clock-deficient) Cry1/Cry2 double knockout mice. PMID: 21858120
    38. Cry1 and Cry2 genes are not an important component of the interval timer. Furthermore, proper calibration of the interval timer does not depend on a functional circadian clock PMID: 21775289
    39. CRY mutation sensitizes p53 mutant and oncogenically transformed cells to tumor necrosis factor alpha -initiated apoptosis by interfacing with the NF-kappaB signaling pathway through the GSK3beta kinase. PMID: 21690409
    40. mice that are arrhythmic because of the deletion of Cry1 and Cry2 clock genes suffer from salt-sensitive hypertension PMID: 21286865
    41. The present results suggest that Cry1 and Cry2 genes are required for regulating pineal melatonin synthesis via circadian and photic signals from the suprachiasmatic nucleus of the hypothalamus. PMID: 20825493
    42. Data suggest that phase delay in Cry1 transcription is required for mammalian clock function. PMID: 21236481
    43. show that Creb activity during fasting is modulated by Cry1 and Cry2, which are rhythmically expressed in the liver PMID: 20852621
    44. CRY1 binding to the Dbp promoter region delayed BMAL1 and CLOCK-mediated transcription of Dbp compared with Rev-Erbalpha PMID: 20551177
    45. The circadian oscillation of XPA is achieved both by regulation of transcription by the core circadian clock proteins including cryptochrome and by regulation at the posttranslational level by the HERC2 ubiquitin ligase. PMID: 20304803
    46. Circadian rhythm plays an important role in the pathogenesis of arthritis; a novel regulatory cross talk between circadian clock gene Cry1 and proinflammatory cytokine tumor necrosis factor-alpha is demonstrated in mouse models of experimental arthritis. PMID: 20042581
    47. The results suggested that epigenetic mechanisms such as DNA methylation might contribute to the developmental expression of clock genes such as mPer1, mPer2, and mCry1. PMID: 20043880
    48. Cry-null mice have dysregulated Hsd3b6, enhanced aldosterone production, and salt-sensitive hypertension PMID: 20023637
    49. cryptochrome 1-deficient mice lack circadian electrical activity in the suprachiasmatic nuclei PMID: 12121621
    50. role in pupillary light response PMID: 12522242

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Note=Transloctaed to the nucleus through interaction with other clock proteins such as PER2 or ARNTL/BMAL1.
  • 蛋白家族：
    DNA photolyase class-1 family
  • 组织特异性：
    Expressed in cones, amacrine cells, and retinal ganglion cells of the retina (at protein level). Expressed in all tissues examined including heart, brain, spleen, lung, liver, skeletal muscle, kidney and testis. Higher levels in brain, liver and testis. I
  • 数据库链接：

    KEGG: mmu:12952

    STRING: 10090.ENSMUSP00000020227

    UniGene: Mm.26237

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