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  4. Recombinant Mouse Cholesterol side-chain cleavage enzyme, mitochondrial (Cyp11a1),Biotinylated

Recombinant Mouse Cholesterol side-chain cleavage enzyme, mitochondrial (Cyp11a1),Biotinylated

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  • 中文名称：
    Recombinant Mouse Cholesterol side-chain cleavage enzyme, mitochondrial(Cyp11a1),Biotinylated
  • 货号：
    CSB-EP886399MO-B
  • 规格：
    ￥2832
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 生物活性：
    Not Test
  • 基因名：
    Cyp11a1
  • Uniprot No.：
    Q9QZ82
  • 别名：
    (CYPXIA1)(Cholesterol desmolase)(Cytochrome P450 11A1)(Cytochrome P450(scc)
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    E.coli
  • 分子量：
    104.4 kDa
  • 表达区域：
    37-526aa
  • 氨基酸序列
    TSTSSPRSFNEIPSPGDNGWLNLYHFWRESGTQKIHYHQMQSFQKYGPIYREKLGTLESVYIVDPKDASILFSCEGPNPERFLVPPWVAYHQYYQRPIGVLFKSSDAWKKDRIVLNQEVMAPGAIKNFVPLLEGVAQDFIKVLHRRIKQQNSGNFSGVISDDLFRFSFESISSVIFGERMGMLEEIVDPEAQRFINAVYQMFHTSVPMLNLPPDFFRLLRTKTWKDHAAAWDVIFNKADEYTQNFYWDLRQKRDFSQYPGVLYSLLGGNKLPFKNIQANITEMLAGGVDTTSMTLQWNLYEMAHNLKVQEMLRAEVLAARRQAQGDMAKMVQLVPLLKASIKETLRLHPISVTLQRYTVNDLVLRNYKIPAKTLVQVASFAMGRDPGFFPNPNKFDPTRWLEKSQNTTHFRYLGFGWGVRQCLGRRIAELEMTILLINLLENFRIEVQNLRDVGTKFSLILMPENPILFNFQPLKQDLGPAVTRKDNTVN
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

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Cyp11a1 Proteins

靶点详情

  • 功能：
    A cytochrome P450 monooxygenase that catalyzes the side-chain hydroxylation and cleavage of cholesterol to pregnenolone, the precursor of most steroid hormones. Catalyzes three sequential oxidation reactions of cholesterol, namely the hydroxylation at C22 followed with the hydroxylation at C20 to yield 20R,22R-hydroxycholesterol that is further cleaved between C20 and C22 to yield the C21-steroid pregnenolone and 4-methylpentanal. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin).
  • 基因功能参考文献：
    1. melatonin not only elevated progesterone (P) secretion, but also upregulated expressions of StAR and Cyp11a1 and also had an increased Ihh expression in endometrium. PMID: 28749439
    2. reduction in expression mirrored reduction in adrenal corticosterone output PMID: 29046340
    3. The results demonstrated that ZnT7 gene silencing downregulated the expression of StAR, P450scc and 3beta-HSD as well as progesterone concentrations in the human chorionic gonadotrophin-stimulated Leydig tumor cells. PMID: 27121848
    4. 1,25D3 modulates CD8+ T cell phenotype via recruitment of the VDR transcription factor to the promoter region of Cyp11a1 leading to prevention of lung allergic responses. PMID: 26750596
    5. Data from Cyp11a1 transgenic mice suggest that overexpression of Cyp11a1 results in reduced pregnancy rate, impaired implantation/placentation, decreased litter size, and stillbirth; overexpression of Cyp11a1 disrupts development of corpus luteum. PMID: 23966322
    6. Cyp11a1 plays an important role in the development of peanut allergy, regulating peanut-induced allergic responses through effects on steroidogenesis, an essential pathway in T(H)2 differentiation. PMID: 23870673
    7. Cyp11a1 controls the phenotypic conversion of CD8(+) T cells from IFN-gamma to IL-13 production, linking steroidogenesis in CD8(+) T cells, a nonclassical steroidogenic tissue, to a proallergic differentiation pathway PMID: 23630275
    8. Sodium fluoride inhibited expression of StAR and P450scc in mouse Leydig tumor cells, which inhibited progesterone secretion. PMID: 22443068
    9. LH/hCG tightly regulates MKP-2 expression, which modulates the induction of CYP11A1 by 8Br-cAMP. PMID: 23471219
    10. Cyp11a1 null embryos had adrenal medulla with abnormal mitochondria. Cells did not synthesized no epinephrine. Decreased diencephalon Hsd11b1, increased Crh, and increased pituitary Pomc expression, thus higher plasma ACTH PMID: 22962254
    11. Data suggest that the 5' flanking region of CYP11A gene contains sequences between 2.5 and 5 kb that are necessary for expression of mouse CYP11A in Leydig cells but not in adrenal cells. [REVIEW] PMID: 22217834
    12. Daxx, a HIPK kinase substrate in the apoptosis pathway, was phosphorylated by HIPK3 at Ser-669 in response to cAMP stimulation. PMID: 22199361
    13. Prenatal exposure to ethanol increased expressions of StAR and P450scc in maternal adrenal and decreased them in fetal adrenal. PMID: 20627497
    14. Pubertal Cd exposure markedly reduced mRNA and protein levels of testicular StAR, P450scc, P450(17alpha) and 17beta-HSD in mice. PMID: 19897027
    15. Transcription of cholesterol side-chain cleavage cytochrome P450 in the placenta: activating protein-2 assumes the role of steroidogenic factor-1 by binding to an overlapping promoter element. PMID: 12145340
    16. Cyp11a1 null mice display a lack of steroid production, abnormal gene expression, and aberrant reproductive organ development resemble various steroid deficiency syndromes. PMID: 12145347
    17. Male CYP11A1 null mice are feminized and their adrenals and genitalia accumulate excessive amounts of lipid PMID: 12530664
    18. Proximal SF-1 binding site plays important role in regulating tissue-specific expression of Cyp11a1 and stress response. PMID: 15666831
    19. This study sought to elucidate overlooked modes of regulation of P450scc transcription in the rodent placenta and ovary. PMID: 17213386
    20. SF-1 and AP-1 cooperate to activate CYP11A1 transcription in vitro and in vivo. PMID: 17218410
    21. Data show that HDAC inhibitors suppressed the expression of steroidogenic gene CYP11A1 and decreased steroid secretion by increasing the ubiquitination and degradation of SF-1, a factor important for the transcription of all steroidogenic genes. PMID: 17709382
    22. Data show that the mutation of the SF-1 response element of Cyp11a1 results in reduced stress response due to decreased adrenal CYP11A1 expression and insufficient stress-induced glucocorticoids secretion. PMID: 18174359
    23. Heterologous expression in yeast of mCYP11A1 fusions with different topogenic signals of yeast mitochondrial proteins was used to gain insight in the mechanism of its topogenesis in mitochondria. PMID: 18621100
    24. Lycopene protects against PCB-induced changes in Leydig cellular StAR protein, cytochrome P450 scc expression and activity of 3beta-HSD. PMID: 19071209

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  • 亚细胞定位：
    Mitochondrion inner membrane; Peripheral membrane protein.
  • 蛋白家族：
    Cytochrome P450 family
  • 数据库链接：

    KEGG: mmu:13070

    STRING: 10090.ENSMUSP00000034874

    UniGene: Mm.302865

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