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  4. Recombinant Mouse Arachidonate 12-lipoxygenase, 12S-type (Alox12)

Recombinant Mouse Arachidonate 12-lipoxygenase, 12S-type (Alox12)

  • 中文名称：
    Recombinant Mouse Arachidonate 12-lipoxygenase, 12S-type(Alox12)
  • 货号：
    CSB-YP001617MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Mouse Arachidonate 12-lipoxygenase, 12S-type(Alox12)
  • 货号：
    CSB-EP001617MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Mouse Arachidonate 12-lipoxygenase, 12S-type(Alox12)
  • 货号：
    CSB-EP001617MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Mouse Arachidonate 12-lipoxygenase, 12S-type(Alox12)
  • 货号：
    CSB-BP001617MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Mouse Arachidonate 12-lipoxygenase, 12S-type(Alox12)
  • 货号：
    CSB-MP001617MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Alox12
  • Uniprot No.：
    P39655
  • 别名：
    Alox12; Alox12pArachidonate 12-lipoxygenase; 12S-type; 12S-LOX; 12S-lipoxygenase; EC 1.13.11.31; Arachidonate 15-lipoxygenase,15S-type; EC 1.13.11.33; Linoleate 13S-lipoxygenase; EC 1.13.11.12; Lipoxin synthase 12-LO; EC 3.3.2.-; Platelet-type lipoxygenase 12; P-12LO
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-663
  • 氨基酸序列
    MGRYRVRVVT GAWLFSGSLN LVRLWLVGEH REAKLELQLR PARGKEEEFD FDVPEDLGPL QFVKLHKQHT VVDDAWFCNL ITVQGPGTSA EAVFPCYRWV QGEGILSLPE GTARLAGDNA LDVFQKYREK ELKERQQTYC WATWKEGLPQ TIAADCKDDL PPNMRFHEEK RLDFEWTLKA GVLEMGLKRV YTLLRSWNHL EDFDQIFWGQ KSALAEKVHQ CWQEDELFGY QFLNGANPML LRRSTSLPSR LVLPSGMEEL QAQLEKELKN GSLFEADFIL LDGIPANVIR GEPQYLAAPL VMLRMDPGGK LLPMAIQIQP PNPSSPAPTL FLPSDPPLAW LLAKIWVRNS DFQLQELQFH LLNTHLVAEV IAVATMRCLP GLHPIFKLLV PHIRYTMEIN TRSRTQLISD GGIFDQVVST GGGGHVQLLT RAVAQLTYHS LCPPDDLANR GLLRIPSALY ARDALQLWEV TARYVKGMVH LFYQSDDIVR GDPELQAWCR EITEVGLCHA QDRGFPVSFQ SRAQLCHFLT MCVFTCTAQH AAINQGQLDW YGWVPNAPCT MRMPPPTSKD DVTMETVMGS LPDVQKACLQ MTITWHLGRL QPDMVPLGHH TEKYFSDPRT KAVLSQFQAD LDNLEKEITA RNEQLDLPYE YLKPSRIENS ITI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the regio and stereo-specific incorporation of molecular oxygen into free and esterified polyunsaturated fatty acids generating lipid hydroperoxides that can be further reduced to the corresponding hydroxy species. Mainly converts arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate) to the specific bioactive lipid (12S)-hydroperoxyeicosatetraenoate/(12S)-HPETE. Through the production of bioactive lipids like (12S)-HPETE it regulates different biological processes including platelet activation. It can also catalyze the epoxidation of double bonds of polyunsaturated fatty acids such as (14S)-hydroperoxy-docosahexaenoate/(14S)-HPDHA resulting in the formation of (13S,14S)-epoxy-DHA. Furthermore, it may participate in the sequential oxidations of DHA ((4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate) to generate specialized pro-resolving mediators (SPMs) like resolvin D5 ((7S,17S)-diHPDHA) and (7S,14S)-diHPDHA, that actively downregulate the immune response and have anti-aggregation properties with platelets. An additional function involves a multistep process by which it transforms leukotriene A4/LTA4 into the bioactive lipids lipoxin A4/LXA4 and lipoxin B4/LXB4, both are vasoactive and LXA4 may regulate neutrophil function via occupancy of specific recognition sites. Can also peroxidize linoleate ((9Z,12Z)-octadecadienoate) to (13S)-hydroperoxyoctadecadienoate/ (13S-HPODE). Due to its role in regulating both the expression of the vascular endothelial growth factor (VEGF, an angiogenic factor involved in the survival and metastasis of solid tumors) and the expression of integrin beta-1 (known to affect tumor cell migration and proliferation), it can be regarded as protumorigenic. Important for cell survival, as it may play a role not only in proliferation but also in the prevention of apoptosis in vascular smooth muscle cells.
  • 基因功能参考文献：
    1. 12(S)-HETrE, a 12-lipoxygenase oxylipin of dihomo-gamma-linolenic acid, inhibits thrombosis via Galphas signaling in platelets. PMID: 27470510
    2. the amount of LPL expressed in muscle and heart governed both the binding of chylomicron particles and the assimilation of chylomicron lipids in the tissue. PMID: 25598081
    3. Data show that the coordinated action of phospholipase A2 IIA (sPLA2-IIA) and 12-lipoxygenase (12-LO) promotes inflammatory arthritis. PMID: 26106157
    4. IGF-1 reduces lipid oxidation and foam cell formation via downregulation of 12/15-LOX to prevent atherosclerosis. PMID: 25549319
    5. Data indicate that 12/15-LO knockout (KO) mice were protected from high fat (HF) diet with high dietary omega6/omega3 ratio (11ratio1, HFH)-induced fatty liver. PMID: 25251155
    6. These results suggest that cardiac 12/15-LOX-induced inflammation and oxidative stress are involved in the development of diabetic cardiomyopathy and that inhibition of 12/15-LOX could be a novel treatment for this condition. PMID: 25187369
    7. EET-heme oxygenase-1 crosstalk as an important cytoprotective mechanism in the amelioration of vascular and adipocyte dysfunction resulting from diet-induced obesity. PMID: 25245389
    8. Overexpression of 15-lipoxygenase increases anxiety behavior in female mice. PMID: 24300237
    9. Findings suggest 12/15-LOX expressed in non-epithelial cells such as macrophages and fibroblasts leads to bronchial epithelial injury. PMID: 23528921
    10. Data indicate that intravenous injection of B16F10 melanoma cells caused lung nodule formation, which was markedly attenuated in 12/15-Lipoxygenase (12/15-LOX) null mice. PMID: 23664097
    11. Following middle cerebral artery occlusion to induce stroke, immunohistochemistry shows both Sema3A and 12/15-LOX are increased in the cortex up to 2 wk. PMID: 23070608
    12. ALOX12 may have a role in growth of breast cancer, and its inhibition may be a strategy for inhibiting tumor growth PMID: 22384268
    13. IT delivery of 12-LOX metabolites of arachidonic acid 12(S)-HpETE, 12(S)-HETE, HXA(3), or HXB(3) evoked profound, persistent tactile allodynia, but 12(S)-HpETE and HXA(3) produced relatively modest, transient heat hyperalgesia PMID: 22493235
    14. These data indicate that 12/15-LO and its metabolites have a prominent antifibrotic role during dermal fibrosis. PMID: 22267335
    15. reduction of alox12 activity or expression could provide a new therapeutic target to reduce ER stress and PMID: 22215650
    16. Arachidonic acid epoxygenase and 12(S)-lipoxygenase jointly produce a hitherto uncharacterized compound acting as oxygen messenger in the ductus arteriosus. PMID: 21619415
    17. Increased expression of 12/15LO in the vessel wall enhances Id3-dependent cell proliferation, fibronectin deposition, and neointimal formation in response to carotid artery injury. PMID: 20947825
    18. 12/15-LOX and AIF are sequential actors in a common cell death pathway that may contribute to stroke-induced brain damage. PMID: 20068575
    19. In the developing epidemis, DeltaNp63alpha directly induces the expression of Alox12, a gene involved in epidermal barrier formation, by interacting with a response element in the promoter of Alox12. PMID: 19555433
    20. 12/15LO mediates early stages of adipose tissue inflammation and whole body insulin resistance induced by high fat feeding PMID: 19787041
    21. Data show that GHRP-2 increased circulating IGF-I and decreased circulating interferon-gamma and aortic gene expression of 12/15-lipoxygenase in ApoE(-/-) mice. PMID: 19819949
    22. selective interleukin-12 synthesis defect in 12/15-lipoxygenase-deficient macrophages associated with reduced atherosclerosis in a mouse model of familial hypercholesterolemia PMID: 12122008
    23. 12-HETE mediates cytokine-induced COX-2 gene transcription and resultant PGE2 production in pancreatic beta-cells PMID: 12198250
    24. activated and induced in peritoneal macrophages PMID: 12664580
    25. mediates monocyte/endothelial cells interactions in the vessel wall in atherogenesis at least in part through molecular regulation of expression of endothelial adhesion molecules PMID: 14676201
    26. 12/15-LO deficiency conferred a significantly worse disease course in experimental allergic encephalomyelitis PMID: 15328042
    27. 12/15-LO activation has roles in macrophage and vascular smooth muscle cell oxidant stress and inflammation PMID: 15576842
    28. both platelet (P) and leukocyte (L)-type 12-LOX are expressed in a distal convoluted tubule cell line PMID: 15610241
    29. the role of 12/15-lipoxygenase in atherogenesis may not be restricted to oxidative LDL modification; Expression of this lipid-peroxidizing enzyme may impact both lipid uptake and intracellular lipid turnover PMID: 15681297
    30. mouse 12/15-LOX (Alox15) and its products, LXA(4) and NPD1 may have roles in wound healing that are distinct from their well established anti-inflammatory properties PMID: 15708862
    31. The suppressive activity of antiproliferative myeloid suppressor cells (MSC) in the peritoneal cavity of mice infected with Taenia crassiceps depends on a switch from nitric oxide- to 12-15-lipoxygenase-dependent alternative activation MSC phenotype. PMID: 15879104
    32. results indicate for the first time that 12/15-LO and COX-2 pathways can cross-talk and activate each other in mesangial cells; these novel interactions may amplify their effects on the progression of diabetic nephropathy PMID: 16514433
    33. The substrate preference of mouse eLOX3 and the unique occurrence of an 8S-LOX enzyme in mouse skin point to a potential LOX pathway for the production of epoxyalcohol in murine epidermal differentiation. PMID: 17045234
    34. iPLA(2)-catalyzed AA release and its metabolism by 12/15-LOX have roles in coupling SR-A-mediated macrophage adhesion to Rac and Cdc42 activation PMID: 17873277
    35. Results suggest a potentially important mechanism linking 12/15-LO activation to MCP-1 expression that induces inflammatory cell infiltration. PMID: 18296557
    36. Role of Src tyrosine kinase in the atherogenic effects of the 12/15-lipoxygenase pathway in vascular smooth muscle cells. PMID: 19095999
    37. 12-Lipoxygenase-deficient mice were significantly protected against GSSG-induced lesion of the cortex in vivo. PMID: 19373248
    38. A genetic strategy was used to disrupt both 12/15-LO and 5-LO on an apolipoprotein E (apoE) atherosclerosis-susceptible background to study the impact of dual LO blockade in atherosclerosis and inflammation. PMID: 19509298
    39. 12/15-LO and 12/15-LO-derived mediators play a prominent role in a regulatory feedback loop which counteracts the exacerbation of inflammation and associated tissue damage during chronic inflammatory disorders such as arthritis. PMID: 19675173
    40. 12/15LO regulates ABCG1 expression and function through p38- and JNK2-dependent mechanisms. PMID: 19713213
    41. 12/15-lipoxygenase-derived 12-hydroxyeicosatetraenoic acid (12-HETE) is a key mediator of vascular permeability in acute lung injury. PMID: 19752233
    42. The enhanced susceptibility of 12/15-LOX-deficient mice to chronic toxoplasmosis was associated with reduced production of IL-12 and gamma interferon. PMID: 19822654

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  • 亚细胞定位：
    Cytoplasm, cytosol. Membrane.
  • 蛋白家族：
    Lipoxygenase family
  • 组织特异性：
    Found primarily in platelets and in microsomal and cytosolic fractions of the epidermis (at protein level).
  • 数据库链接：

    KEGG: mmu:11684

    STRING: 10090.ENSMUSP00000000329

    UniGene: Mm.12286

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