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  4. Recombinant Mouse Adenosine deaminase (Ada)

Recombinant Mouse Adenosine deaminase (Ada)

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  • 中文名称：
    Recombinant Mouse Adenosine deaminase (Ada)
  • 货号：
    CSB-EP001268MO
  • 规格：
    ￥1344
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 95% as determined by SDS-PAGE.
  • 生物活性：
    Not Test
  • 基因名：
    Ada
  • Uniprot No.：
    P03958
  • 别名：
    Adenosine aminohydrolase
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    E.coli
  • 分子量：
    46.8 kDa
  • 表达区域：
    2-352aa
  • 氨基酸序列
    AQTPAFNKPKVELHVHLDGAIKPETILYFGKKRGIALPADTVEELRNIIGMDKPLSLPGFLAKFDYYMPVIAGCREAIKRIAYEFVEMKAKEGVVYVEVRYSPHLLANSKVDPMPWNQTEGDVTPDDVVDLVNQGLQEGEQAFGIKVRSILCCMRHQPSWSLEVLELCKKYNQKTVVAMDLAGDETIEGSSLFPGHVEAYEGAVKNGIHRTVHAGEVGSPEVVREAVDILKTERVGHGYHTIEDEALYNRLLKENMHFEVCPWSSYLTGAWDPKTTHAVVRFKNDKANYSLNTDDPLIFKSTLDTDYQMTKKDMGFTEEEFKRLNINAAKSSFLPEEEKKELLERLYREYQ
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    C-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

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Ada Proteins

靶点详情

  • 功能：
    Catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion. Enhances dendritic cell immunogenicity by affecting dendritic cell costimulatory molecule expression and cytokines and chemokines secretion. Enhances CD4+ T-cell differentiation and proliferation. Acts as a positive modulator of adenosine receptors ADORA1 and ADORA2A, by enhancing their ligand affinity via conformational change. Stimulates plasminogen activation. Plays a role in male fertility. Plays a protective role in early postimplantation embryonic development.
  • 基因功能参考文献：
    1. Pulmonary alveolar proteinosis in adenosine deaminase-deficient mice PMID: 24439080
    2. Dietary regulation of adenosine deaminase activity in stomach, small intestine and spleen of mice PMID: 22905401
    3. Data indicate that untreated murine adenosine deaminase ADA(-/-) Tregs show alterations in the plasma membrane CD39/CD73. ectonucleotidase machinery and limited suppressive activity via extracellular adenosine. PMID: 22184407
    4. ADA is upregulated in NOD dendritic cells, which results in their exuberant and spontaneous activation, which triggers autoimmune T cell activation. PMID: 21593382
    5. The catalytic mechanism of Mus musculus adenosine deaminase (ADA) has been studied by quantum mechanics and two-layered ONIOM calculations PMID: 20575011
    6. Adenosine deaminase is necessary but not sufficient to confer puromycin aminonucleoside sensitivity to podocytes. PMID: 20649959
    7. adenosine deaminase deficient results in deoxy-ATP accumulation and leads to reduced thymic T cell production PMID: 12163459
    8. Transactivation by a duodenum-specific enhancer requires functional GATA4 binding sites PMID: 12571085
    9. ADA-deficient B cells exhibit a higher propensity to undergo B cell receptor-mediated apoptosis than their wild-type counterparts, and suggest a role for ADA in the survival of cells during antigen-dependent responses. PMID: 14607964
    10. This study provides a basis for investigating the folding kinetics of murine ADA and folding mechanisms of (beta/alpha)8-barrel proteins using 19F NMR sepctroscopy. PMID: 14769019
    11. RUNX1 transcription factor plays a significant role in regulating Ada gene expression in the trophoblast cell lineage. PMID: 16338472
    12. These findings suggest that the A(2A)R plays a protective role in the ADA-deficient model of pulmonary inflammation. PMID: 17601796
    13. Progesterone may have an antiseizure effect with the additional finding that decreased levels of adenosine deaminase that would have resulted in increased adenosine levels that exerts anticonvulsant effect via GABA-A receptors. PMID: 19125374
    14. ADA/A2B adenosine receptor double-knockout mice exhibited enhanced pulmonary inflammation and airway destruction PMID: 19494329
    15. ADA-deficient SCID is associated with a specific microenvironment and bone phenotype characterized by RANKL/OPG imbalance and osteoblast insufficiency. PMID: 19633200

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  • 亚细胞定位：
    Cell membrane; Peripheral membrane protein; Extracellular side. Cell junction. Cytoplasmic vesicle lumen. Cytoplasm. Lysosome.
  • 蛋白家族：
    Metallo-dependent hydrolases superfamily, Adenosine and AMP deaminases family
  • 组织特异性：
    Detected in brain neurons in the median emninence (at protein level). Expressed in secondary deciduum (at protein level). Found in all tissues, occurs in large amounts in T-lymphocytes and, at the time of weaning, in gastrointestinal tissues.
  • 数据库链接：

    KEGG: mmu:11486

    STRING: 10090.ENSMUSP00000017841

    UniGene: Mm.388

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