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  4. Recombinant Mouse 85 kDa calcium-independent phospholipase A2 (Pla2g6), partial

Recombinant Mouse 85 kDa calcium-independent phospholipase A2 (Pla2g6), partial

  • 中文名称：
    小鼠Pla2g6重组蛋白
  • 货号：
    CSB-YP018104MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Pla2g6重组蛋白
  • 货号：
    CSB-EP018104MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Pla2g6重组蛋白
  • 货号：
    CSB-EP018104MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Pla2g6重组蛋白
  • 货号：
    CSB-BP018104MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Pla2g6重组蛋白
  • 货号：
    CSB-MP018104MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Pla2g6
  • Uniprot No.：
    P97819
  • 别名：
    Pla2g6; Pnpla9; 85/88 kDa calcium-independent phospholipase A2; CaI-PLA2; EC 3.1.1.4; Group VI phospholipase A2; GVI PLA2; Intracellular membrane-associated calcium-independent phospholipase A2 beta; iPLA2-beta; Patatin-like phospholipase domain-containing protein 9; PNPLA9
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Calcium-independent phospholipase involved in phospholipid remodeling with implications in cellular membrane homeostasis, mitochondrial integrity and signal transduction. Hydrolyzes the ester bond of the fatty acyl group attached at sn-1 or sn-2 position of phospholipids (phospholipase A1 and A2 activity respectively), producing lysophospholipids that are used in deacylation-reacylation cycles. Hydrolyzes both saturated and unsaturated long fatty acyl chains in various glycerophospholipid classes such as phosphatidylcholines, phosphatidylethanolamines and phosphatidates, with a preference for hydrolysis at sn-2 position. Can further hydrolyze lysophospholipids carrying saturated fatty acyl chains (lysophospholipase activity). Upon oxidative stress, contributes to remodeling of mitochondrial phospholipids in pancreatic beta cells, in a repair mechanism to reduce oxidized lipid content. Preferentially hydrolyzes oxidized polyunsaturated fatty acyl chains from cardiolipins, yielding monolysocardiolipins that can be reacylated with unoxidized fatty acyls to regenerate native cardiolipin species. Hydrolyzes oxidized glycerophosphoethanolamines present in pancreatic islets, releasing oxidized polyunsaturated fatty acids such as hydroxyeicosatetraenoates (HETEs). Has thioesterase activity toward fatty-acyl CoA releasing CoA-SH known to facilitate fatty acid transport and beta-oxidation in mitochondria particularly in skeletal muscle. Plays a role in regulation of membrane dynamics and homeostasis. Selectively hydrolyzes sn-2 arachidonoyl group in plasmalogen phospholipids, structural components of lipid rafts and myelin. Regulates F-actin polymerization at the pseudopods, which is required for both speed and directionality of MCP1/CCL2-induced monocyte chemotaxis. Targets membrane phospholipids to produce potent lipid signaling messengers. Generates lysophosphatidate (LPA, 1-acyl-glycerol-3-phosphate), which acts via G-protein receptors in various cell types. Has phospholipase A2 activity toward platelet-activating factor (PAF, 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine), likely playing a role in inactivation of this potent proinflammatory signaling lipid. In response to glucose, amplifies calcium influx in pancreatic beta cells to promote INS secretion.
  • 基因功能参考文献：
    1. Disturbances in mitochondrial potential and changes in calcium signaling are dependent on iPLA2 activity in an animal model of infantile neuroaxonal dystrophy. PMID: 27395788
    2. The antinociceptive effect of maprotiline was abrogated by iPLA2 antisense oligonucleotide injection to the prefrontal cortex, indicating a role of this enzyme in antinociception. In contrast, injection of iPLA2 antisense oligonucleotide to the somatosensory cortex did not reduce the antinociceptive effect of maprotiline. PMID: 27789386
    3. The absence of iPLA2beta promotes macrophage M2 polarization. PMID: 27650501
    4. Time lapse microscopy and integrin endocytosis assay revealed the essential role of calcium-independent phospholipase A2 activity for the recycling of alpha 6 integrin-GFP from the endosomal recycling complex to the plasma membrane. PMID: 27655917
    5. iPLA2-gamma has a protective functional role in the normal glomerulus and in glomerulonephritis. PMID: 27226532
    6. Results suggest that the degeneration of dopaminergic neurons occurs mainly in the distal region of axons in iPLA2beta-KO mice. PMID: 27078024
    7. This study showed that the absence of iPLA2beta activity does not influence myocardial inflammation, but iPLA2beta is essential for T. cruzi clearance. PMID: 26857573
    8. Results support the deleterious role of iPLA2beta in severe obesity associated NAFLD. PMID: 26873633
    9. Genetic or molecular impairment of PLA2g6-dependent Ca(2+) signalling is a trigger for autophagic dysfunction, progressive loss of dopaminergic (DA) neurons in substantia nigra pars compacta and age-dependent L-DOPA-sensitive motor dysfunction. PMID: 26755131
    10. An inactivation of iPLA2beta exacerbated pathogenesis of experimental colitis by promoting intestinal epithelial cell apoptosis, inhibiting crypt cell regeneration, and causing damage to mucus barrier allowing an activation of innate immune response. PMID: 26182903
    11. diapocynin inhibited ROS production, abolished iPLA2 activity and reduced Ca2+ influx through stretch-activated and store-operated channels, two major pathways responsible for excessive Ca2+ entry in dystrophic muscle PMID: 25329652
    12. Mice deficient in iPLA2Beta show evidence of neuroinflammation and motor dysfunction in later life. PMID: 24919816
    13. membrane potential sensitive activity is the iPLA2gamma PMID: 26206229
    14. In this study we demonstrate for the first time that corticostriatal LTP is dependant on iPLA2 signalling, which can be reversed with application of DHA. PMID: 25562715
    15. Enteropathy may result from acute autoimmune hepatitis in a susceptible host such as iPLA2beta deficiency. PMID: 25957555
    16. these data indicate that beta-cell apoptosis is, in part, attributable to the modulation of 5'SS selection in the Bcl-x pre-mRNA by bioactive lipids modulated by iPLA2beta. PMID: 25762722
    17. The loss of PS2 could have a critical role in lung tumor development through the upregulation of iPLA2 activity by reducing gamma-secretase. PMID: 24858037
    18. This study suggests that iPLA2beta-derived lipid signals modulate immune cell responses, raising the possibility that early inhibition of iPLA2beta may be beneficial in ameliorating autoimmune destruction of beta-cells and mitigating type 1 diabetes. PMID: 25213337
    19. iPLA2beta confers significant protection of beta-cells against C16:0-induced injury PMID: 24648512
    20. The significance of calcium-independent phospholipase A, group VIA (iPLA2-VIA), in retinal pigment epithelial cell survival, was investigated. PMID: 24791136
    21. Contrary to cPLA2alpha, iPLA2beta spares arachidonate-containing phospholipids. PMID: 24337743
    22. These findings suggest that alpha2AP regulates the development of skin fibrosis by PGF(2alpha) synthesis through ATGL/iPLA(2). PMID: 23124680
    23. a genetic iPLA(2)beta deficiency in mice is associated with reduced DHA metabolism, profound changes in lipid-metabolizing enzyme expression (demonstrating lack of redundancy) and of phospholipid fatty acid content of brain (particularly of DHA) PMID: 22349267
    24. The present study confirms the involvement of iPLA(2)-VIA in efficient retinal pigment epithelium phagocytosis of photoreceptor outer segments. PMID: 22680611
    25. identify iPLA(2)gamma as an important mechanistic component of the mPTP, define its downstream products as potent regulators of mPTP opening PMID: 22778252
    26. genetic ablation of PLA2G6 leads to the development of cerebellar atrophy, exhibiting significant loss of Purkinje cells, and reactive astrogliosis, the activation of microglial cells, and up-regulation of the pro-inflammatory cytokines TNF-alpha and IL-1beta. PMID: 22046428
    27. Data show that Pla2g6 mutant mice develop pathology analogous to that observed in infantile neuroaxonal dystrophy (INAD) patients. PMID: 22442204
    28. mitochondrial iPLA(2)gamma is activated by divalent cations and inhibited by acyl-CoA modulating the generation of biologically active metabolites that regulate mitochondrial bioenergetic and signaling functions. PMID: 22389508
    29. iPLA(2)beta up-regulation exacerbates angiotensin II-induced vascular smooth muscle cell hypertrophy. PMID: 22363752
    30. acts as an inhibitory modulator of NKCC2 activity in thick ascending limb PMID: 22218592
    31. Cigarette smoke extract (CSE)inhibited platelet-activating factor-acetylhydrolase activity of lung endothelial cells isolated from wild-type (WT) and iPLA2 beta knockout mice, and with WT cells, CSE enhanced PAF production and RAW 264.7 cell adherence. PMID: 21984569
    32. Analysis of two major intracellular phospholipases A(2) (PLA(2)) in mast cells reveals crucial contribution of cytosolic PLA(2)alpha, not Ca(2+)-independent PLA(2)beta, to lipid mobilization in proximal mast cells and distal fibroblasts. PMID: 21880721
    33. neuroaxonal dystrophy in iPLA2beta deficiency is thought to be caused by two types of pathomechanism, which are associated with insufficient remodeling of the mitochondrial inner membrane and presynaptic membrane of axon terminals PMID: 21813701
    34. These data indicate that endothelial cell iPLA(2)beta is responsible for platelet-activating factor production and adherence of E0771 cells and may play a role in cancer cell migration to distal locations. PMID: 21228317
    35. iPLA(2)beta deficiency reduces brain docosahexaenoic acid metabolism and signaling PMID: 20686114
    36. endoplasmic reticulum stress has effects on group VIA phospholipase A2 in beta cells that include tyrosine phosphorylation and increased association with calnexin PMID: 20732873
    37. endothelial PAF production is entirely dependent on iPLA(2)beta activity PMID: 20521843
    38. iPLA(2)beta is an important mediator of vascular relaxation and intracellular calcium homeostasis in both smooth muscle and endothelial cells. PMID: 20382858
    39. inhibition of iPLA2, lysophospholipid production and action may be of great interest to reduce Ca2+ entry and downstream degeneration of dystrophic muscle. PMID: 19914213
    40. These findings support an RNA-dependent protein kinase-independent signaling role for iPLA(2) in the antiviral response of macrophages and the stimulation of inducible nitric oxide synthase expression. PMID: 12167650
    41. iPLA(2)beta and iPLA(2)gamma are required for the adipogenic program that drives resting 3T3-L1 cells into adipocytes after hormone stimulation PMID: 15024020
    42. iPLA2beta has a signaling role in virus-induced iNOS expression by macrophages [Ipla2(beta)] PMID: 15946940
    43. iPLA(2)beta-null mice exhibit phenotypic abnormalities in pancreatic islets in addition to testes and macrophages PMID: 16732058
    44. Enhanced store-operated Ca2+ entry in dystrophic fibers is likely to be due to a near twofold overexpression of Ca2+-independent phospholipase A2. PMID: 16926189
    45. group VIA phospholipase A2 (iPLA2beta) plays a pivotal role in Ang II-induced RGS2 mRNA up-regulation in vascular smooth muscle cells PMID: 17613534
    46. iPLA(2)beta participates in ER stress-induced macrophage apoptosis caused by free cholesterol loading or thapsigargin. PMID: 17627946
    47. iPLA(2)-catalyzed AA release and its metabolism by 12/15-LOX have roles in coupling SR-A-mediated macrophage adhesion to Rac and Cdc42 activation PMID: 17873277
    48. islets from male iPLA(2)beta-null mice exhibit blunted insulin secretion PMID: 17895289
    49. Store-operated channels are activated via an iPLA2-dependent pathway and may produce a depolarization of smooth muscle cells PMID: 18156193
    50. Neuroaxonal dystrophy caused by group VIA phospholipase A2 deficiency in mice. PMID: 18305254

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  • 亚细胞定位：
    Cytoplasm. Cell membrane. Mitochondrion. Cell projection, pseudopodium.
  • 组织特异性：
    Expressed in neurons of central and peripheral nervous system. Highly expressed in Purkinje cells in cerebellum and dorsal and ventral horn neurons in the spinal cord. Expressed in testis (at protein level). Expressed in skeletal muscle (at protein level)
  • 数据库链接：

    KEGG: mmu:53357

    STRING: 10090.ENSMUSP00000134672

    UniGene: Mm.155620

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