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  4. Recombinant Human parvovirus B19 Non-capsid protein NS-1 (NS1), partial

Recombinant Human parvovirus B19 Non-capsid protein NS-1 (NS1), partial

  • 中文名称：
    Recombinant Human parvovirus B19 Non-capsid protein NS-1(NS1) ,partial
  • 货号：
    CSB-YP357083HPM
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Human parvovirus B19 Non-capsid protein NS-1(NS1) ,partial
  • 货号：
    CSB-EP357083HPM-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Human parvovirus B19 Non-capsid protein NS-1(NS1) ,partial
  • 货号：
    CSB-BP357083HPM
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Human parvovirus B19 Non-capsid protein NS-1(NS1) ,partial
  • 货号：
    CSB-MP357083HPM
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    NS1
  • Uniprot No.：
    P07298
  • 别名：
    NS1; Initiator protein NS1; NS1; EC 3.1.21.-; EC 3.6.4.12; NCVP1; Non-capsid protein NS-1; Non-structural protein 1; Non-structural protein NS1
  • 种属：
    Human parvovirus B19 (isolate AU) (HPV B19)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

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问答及客户评论

■ 常见问题解答
Q:

Can you recommend a EBV-/Parvovirus - Peptide/Protein for use in cell culture (T-cell proliferation assay)
Here are the information provided for your reference:
- Searching for EBV-Proteins/Peptides and B19-ProteinsPeptides for use in cell culture (T-cell proliferation assay)
- Name/specific Target: EBV: EA, VCA, EBNA1
- Origin: if possible Protein/Peptide with viral origin. But E.Coli is OK as well
- Conjugation/Tag: if possible not conjugated, but tag is OK as well
- Size: 20 ug

A:
Thanks for your inquiry.
Recombinant Human parvovirus B19 Non-capsid protein NS-1(NS1) ,partial
CSB-YP357083HPM >> Yeast
CSB-EP357083HPM >> E.coli 3-7working days.
CSB-BP357083HPM >> Baculovirus
CSB-MP357083HPM >> Mammalian cell
Express region:1-255aa；partial
Tag information:
EP,N-terminal 6xHis-SUMO-tagged YP,N-terminal 6xHis-tagged；BP,MP: N-terminal 10xHis-tagged + C-terminal Myc-tagged
Sequence:

MELFRGVLQVSSNVLDCANDNWWCSLLDLDTSDWEPLTHTNRLMAIYLSSVASKLDFTGGPLAGCLYFFQVECNKFEEGYHIHVVTGGPGLNPRNLTVCVEGLFNNVLYHLVTENVKLKFLPGMTTKGKYFRDGEQFIENYLMKKIPLNVVWCVTNIDGYIDTCISATFRRGACHAKKPRITTAINDTSSDAGESSGTGAEVVPFNGKGTKASIKFQTMVNWLCENRVFTEDKWKLVDFNQYTLLSSSHSGSFQI


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靶点详情

  • 功能：
    Multifunctional protein essential for viral DNA replication, which cooperatively interacts with the viral DNA origin of replication and transactivates several promoters including the viral p6 promoter. Binds the origin of replication and performs an endonucleolytic nick within a conserved sequence in the viral genome, thereby initiating the rolling circle replication (RCR). Participates in the transcriptional regulation the viral p6 promoter that regulates all viral transcripts and the cellular CDN1A or IL6 promoters. Transactivates several host promoters some of which induce the S cell cycle phase for the production of host replicative proteins. Upregulates the expression of host E2F4 and E2F5 and interacts with both these factors thereby inhibiting the host cell cycle G2/M transition. This arrest promotes apoptosis for viral release.
  • 亚细胞定位：
    Host nucleus.
  • 蛋白家族：
    Parvoviruses non-capsid protein family

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