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  4. Recombinant Human herpesvirus 1 DNA polymerase catalytic subunit (UL30), partial

Recombinant Human herpesvirus 1 DNA polymerase catalytic subunit (UL30), partial

  • 中文名称：
    Recombinant Human herpesvirus 1 DNA polymerase catalytic subunit (UL30) ,partial
  • 货号：
    CSB-YP366130HWY
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Human herpesvirus 1 DNA polymerase catalytic subunit (UL30) ,partial
  • 货号：
    CSB-EP366130HWY
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Human herpesvirus 1 DNA polymerase catalytic subunit (UL30) ,partial
  • 货号：
    CSB-EP366130HWY-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Human herpesvirus 1 DNA polymerase catalytic subunit (UL30) ,partial
  • 货号：
    CSB-BP366130HWY
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Human herpesvirus 1 DNA polymerase catalytic subunit (UL30) ,partial
  • 货号：
    CSB-MP366130HWY
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    UL30
  • Uniprot No.：
    P04293
  • 别名：
    UL30DNA polymerase catalytic subunit; EC 2.7.7.7; EC 3.1.26.4
  • 种属：
    Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Replicates viral genomic DNA. The replication complex is composed of six viral proteins: the DNA polymerase, processivity factor, primase, primase-associated factor, helicase, and ssDNA-binding protein. Additionally, the polymerase contains an intrinsic ribonuclease H (RNase H) activity that specifically degrades RNA/DNA heteroduplexes or duplex DNA substrates in the 5' to 3' direction. Therefore, it can catalyze the excision of the RNA primers that initiate the synthesis of Okazaki fragments at a replication fork during viral DNA replication.
  • 基因功能参考文献：
    1. Data suggest that UL30/UL42 is a single bifunctional protein exhibiting both polymerase and exonuclease activities; UL30/UL42 also exhibits two completely independent active sites for these activities. PMID: 26836009
    2. The mutation W781V in HSV-1 UL30 induced resistance to foscarnet, acyclovir, and ganciclovir. PMID: 25673718
    3. These results reveal an important role for the conserved motif at residues 44-49 of HSV-1 Pol for ganglionic viral replication. PMID: 24413420
    4. Nucleotide sequence analysis of UL30 for a population of HSV-1 strain L2. PMID: 24466749
    5. results strongly indicate that the conserved motif within the pre-NH(2)-terminal domain is important for viral DNA synthesis and production of infectious virus and indicate a functional role for this domain PMID: 22875965
    6. The DNA pol gene of 53 HSV-1 isolates contained 47 distinct polymorphism-associated amino acid substitutions and 11 substitutions were found in the DNA pol gene of 12 HSV-2 strains. PMID: 22024514
    7. Herpes simplex virus 1 DNA polymerase stimulated the nick translation activity of human Fen-1 on DNA- or RNA-containing primer/templates, indicating that the activities were coordinated. PMID: 20444887
    8. novel inhibition mechanism in which a representative of a series of non-nucleosidic viral polymerase inhibitors, the 4-oxo-dihydroquinolines, binds at the polymerase active site interacting non-covalently with both the polymerase and the DNA duplex PMID: 16638752
    9. Tne nuclear import pathway of polymerase catalytic subunit pUL30 encompasses two putative nuclear localization signals NLS2 and NLS3 necessary for pUL30 nuclear transport that mediate high affinity binding to importin (IMP) heterodimer through IMPalpha. PMID: 17640102
    10. Defined novel point mutations in HSV-1 DNA polymerase affecting neurovirulence/drug resistance. PMID: 17713155
    11. have implications on the role of base excision repair in viral genome maintenance during lytic replication and reactivation from latency PMID: 18695225
    12. This study demonstrates that nuclear transport and localization of the Pol subunit is independent of herpesviral functions, and neither requires the presence of herpesviral DNA sequences. PMID: 19194793
    13. The L774F mutation-containing YD12 Pol supported the growth of viral progeny and replicated DNA efficiently and accurately. PMID: 19420083

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  • 亚细胞定位：
    Host nucleus.
  • 蛋白家族：
    DNA polymerase type-B family
  • 数据库链接：

    KEGG: vg:2703462

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