Recombinant Human adenovirus C serotype 5 Protease (L3)

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中文名称：

Recombinant Human adenovirus C serotype 5 Protease(L3)
货号：

CSB-EP361000HILb1
规格：

￥2328
图片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP361000HILB1 could indicate that this peptide derived from E.coli-expressed Human adenovirus C serotype 5 (HAdV-5) (Human adenovirus 5) L3.

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP361000HILB1 could indicate that this peptide derived from E.coli-expressed Human adenovirus C serotype 5 (HAdV-5) (Human adenovirus 5) L3.
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纯度：

Greater than 90% as determined by SDS-PAGE.
基因名：

L3
Uniprot No.：

P03253
别名：

L3Protease; EC 3.4.22.39; Adenain; Adenovirus protease; AVP; Adenovirus proteinase; Endoprotease
种属：

Human adenovirus C serotype 5 (HAdV-5) (Human adenovirus 5)
蛋白长度：

Full Length
来源：

E.coli
分子量：

28.1kDa
表达区域：

1-204aa
氨基酸序列

MGSSEQELKAIVKDLGCGPYFLGTYDKRFPGFVSPHKLACAIVNTAGRETGGVHWMAFAWNPHSKTCYLFEPFGFSDQRLKQVYQFEYESLLRRSAIASSPDRCITLEKSTQSVQGPNSAACGLFCCMFLHAFANWPQTPMDHNPTMNLITGVPNSMLNSPQVQPTLRRNQEQLYSFLERHSPYFRSHSAQIRSATSFCHLKNM
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
蛋白标签：

N-terminal 10xHis-tagged and C-terminal Myc-tagged
产品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
缓冲液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
储存条件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保质期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
货期：

3-7 business days
注意事项：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
产品描述：

The N-terminal 10xHis tag and C-terminal Myc tag was fused to the gene fragment corresponding to the 1-204aa of the human adenovirus C serotype 5 L3 protein and then was cloned into an expression vector. The expression vector was transformed into the E.coli for expression. The generated product was purified and separated to obtain the recombinant human adenovirus C serotype 5 L3 protein. Its purity is higher than 90%. This recombinant L3 protein showed an apparent molecular weight of about 30 kDa under SDS-PAGE condition.
Cleaves viral precursor proteins including pTP, pIIIa, pVI, pVII, pVIII, and pX could lead to the production of mature virions. Compared to the unmature virions, mature virions show a weakened organization, which further enhances the subsequent uncoating. The particle lacks infectivity and is unable to uncoat as there is no maturation. In the cytoplasm, L3Protease may participate in the cytoskeleton destruction and cleaves host cell cytoskeletal keratins K7 and K18.
Datasheet & COA：

Please contact us to get it.

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功能：

Cleaves viral precursor proteins (pTP, pIIIa, pVI, pVII, pVIII, and pX) inside newly assembled particles giving rise to mature virions. Protease complexed to its cofactor slides along the viral DNA to specifically locate and cleave the viral precursors. Mature virions have a weakened organization compared to the unmature virions, thereby facilitating subsequent uncoating. Without maturation, the particle lacks infectivity and is unable to uncoat. Late in adenovirus infection, in the cytoplasm, may participate in the cytoskeleton destruction. Cleaves host cell cytoskeletal keratins K7 and K18.
亚细胞定位：

Virion. Host nucleus.
蛋白家族：

Peptidase C5 family

Recombinant Human adenovirus C serotype 5 Protease (L3)

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