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  4. Recombinant Human Serine/threonine-protein kinase SMG1 (SMG1), partial

Recombinant Human Serine/threonine-protein kinase SMG1 (SMG1), partial

  • 中文名称：
    人SMG1重组蛋白
  • 货号：
    CSB-YP847208HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人SMG1重组蛋白
  • 货号：
    CSB-EP847208HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人SMG1重组蛋白
  • 货号：
    CSB-EP847208HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人SMG1重组蛋白
  • 货号：
    CSB-BP847208HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人SMG1重组蛋白
  • 货号：
    CSB-MP847208HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    SMG1
  • Uniprot No.：
    Q96Q15
  • 别名：
    61E3.4; ATX; hSMG-1; hSMG1; KIAA0421; Lambda interacting protein; Lambda-interacting protein; Lambda/iota protein kinase C interacting protein; Lambda/iota protein kinase C-interacting protein; LIP; Phosphatidylinositol 3 kinase related kinase; Phosphatidylinositol 3 kinase related protein kinase; PI 3 kinase related kinase SMG 1; Serine/threonine protein kinase SMG1; Serine/threonine-protein kinase SMG1; SMG-1; smg1; SMG1_HUMAN
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Serine/threonine protein kinase involved in both mRNA surveillance and genotoxic stress response pathways. Recognizes the substrate consensus sequence [ST]-Q. Plays a central role in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by phosphorylating UPF1/RENT1. Recruited by release factors to stalled ribosomes together with SMG8 and SMG9 (forming the SMG1C protein kinase complex), and UPF1 to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Also acts as a genotoxic stress-activated protein kinase that displays some functional overlap with ATM. Can phosphorylate p53/TP53 and is required for optimal p53/TP53 activation after cellular exposure to genotoxic stress. Its depletion leads to spontaneous DNA damage and increased sensitivity to ionizing radiation (IR). May activate PRKCI but not PRKCZ.
  • 基因功能参考文献：
    1. Because SMG-1-p53 signaling attenuates production of mitochondrial ROS. PMID: 28749708
    2. ionizing radiation (IR) inhibits the activity of SMG1, a phosphoinositide-3-kinase-like kinase family member, reducing the binding of SMG1 to a specific region near exon 9 of p53 precursor mRNA and promoting the binding of ribosomal protein L26 (RPL26) to p53 pre-mRNA PMID: 28288992
    3. Knock down of the expression of SMG-1 inhibited tumor cell proliferation and induced the chemosensitivity of pancreatic cancer cells in vitro. PMID: 25760059
    4. SMG1 was hypermethylated in 66% of AML samples compared with none in controls. mTOR expression level was negatively correlated to SMG1 expression in AML patients which indicated that SMG1 and mTOR maybe act antagonistically to regulate AML cell growth. PMID: 25257528
    5. A genome-wide RNA interference screen Hepatocellular carcinoma cell lines revealed the role of suppressor of SMG-1 as determinant of sorafenib resistance. PMID: 25017961
    6. SMG1 regulates NMD by recruiting UPF1 and UPF2 to distinct nearby positions, and can form a complex with UPF2 in vivo in an UPF1-independent manner. PMID: 25002321
    7. We conclude that SMG-1 is downregulated in HCC and may represent a promising biomarker for predicting the prognosis of HCC, including the prognosis of early-stage patients. PMID: 24700316
    8. Novel role for SMG-1 is identified in regulating Cdc25A and suppressing oncogenic CDK2 driven proliferation, confirming SMG-1 as a tumor suppressor. PMID: 24107632
    9. This study demonstrated the quantitative regulation of Upf1 and Upf2 proteins by ubiquitin-proteasome system and SMG1. PMID: 24173962
    10. SMG1 protein levels were significantly reduced in brain. PMID: 24204929
    11. Downregulation of SMG1 causes the reduction in the level of Upf1 phosphorylation and delays adipogenesis, suggesting the functional involvement of SMG1 in adipogenesis via SMD. PMID: 24185201
    12. Data indicate that down-regulation of SMG1 expression is mediated by miRNA-125 binding to a microRNA response element in the 3' untranslated region of SMG1 mRNA, which leads to degradation of the SMG1 mRNA. PMID: 23583196
    13. the inflammation observed in Smg1 haploinsufficiency contributes to susceptibility to cancer, and Smg1-deficient animals represent a model of inflammation-enhanced cancer development PMID: 23277562
    14. Levels of SMG-1 expression negatively correlated with HPV status in cancer cell lines and tumors. PMID: 22247495
    15. Hyperglycemia (both 10 and 20 mM)-treated monocyte showed up-regulated phosphorylation of p101 and p110gamma subunits of PI-3 kinase in comparison to 5 mM glucose. PMID: 21307779
    16. stress granule formation appears more complex than originally envisaged and hSMG-1 plays a central role in this process. PMID: 21911475
    17. SMG1 plays independent roles in responding to DNA, RNA damage and a broader role in protection against cellular stress tna other member of the PIKK family. PMID: 21701263
    18. The inhibition of hSMG-1 significantly enhances the sensitivity of human lung cancer H1299 cells to gemcitabine and cisplatin. PMID: 21418860
    19. large-scale conformational changes induced by SMG-8 after SMG-9-mediated recruitment tune SMG-1 kinase activity to modulate nonsense-mediated mRNA decay PMID: 21245168
    20. The results of this study indicated a significant impact of brief naturalistic stressors on AS-mediated regulation of gene expression in peripheral leukocytes, and suggest the SMG-1 splice variant as a potential biomarker for acute psychological stress PMID: 20723581
    21. The CK2 phospho-dependent interaction between TEL2 and the R2TP complex affects phosphatidylinositol 3-kinase-related kinase functions and is essential for mTOR and SMG1 stability in vivo. PMID: 20864032
    22. Expression of hSMG-1 is required for optimal p53 activation after cellular exposure to genotoxic stress, and depletion of hSMG-1 leads to spontaneous DNA damage and increased sensitivity to ionizing radiation. PMID: 15175154
    23. hSMG-1 teams with ATM and ATR to insure the overall quality of the transcriptome in human cells [review] PMID: 15279777
    24. Thus, the SMG-1-mediated phosphorylation of Upf1 occurs on the association of SURF with EJC, which provides the link between the EJC and recognition of PTCs and triggers NMD PMID: 16452507
    25. distantly located N- and C-terminal sequences are essential for the intrinsic kinase activity of SMG-1 PMID: 17229728
    26. hSMG-1 plays an important role in cell survival during TNFalpha-induced stress PMID: 18326048
    27. hSMG-1 and ATM sequentially and independently regulate the G1 checkpoint during oxidative stress PMID: 18332866
    28. SMG-1 negatively regulated HIF-1alpha activity in hypoxia, in part through blocking MAPK activation. PMID: 19406746
    29. SMG-8 and SMG-9 suppress SMG-1 kinase activity in the isolated SMG-1 complex and are involved in Nonsense-mediated mRNA decay in both mammals and nematodes PMID: 19417104
    30. The phosphatidylinositol 3-kinase pathway plays a critical role in the transfer of signal from morphine stimuli to mu opioid receptor gene transcription. PMID: 19765550

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  • 亚细胞定位：
    Nucleus. Cytoplasm.
  • 蛋白家族：
    PI3/PI4-kinase family
  • 组织特异性：
    Widely expressed, with highest level in heart and skeletal muscle. Expressed in placenta, brain, lung and spleen, but not in liver.
  • 数据库链接：

    HGNC: 30045

    OMIM: 607032

    KEGG: hsa:23049

    STRING: 9606.ENSP00000402515

    UniGene: Hs.460179

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