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  4. Recombinant Human Ras-associated and pleckstrin homology domains-containing protein 1 (RAPH1), partial

Recombinant Human Ras-associated and pleckstrin homology domains-containing protein 1 (RAPH1), partial

  • 中文名称：
    人RAPH1重组蛋白
  • 货号：
    CSB-YP761687HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人RAPH1重组蛋白
  • 货号：
    CSB-EP761687HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人RAPH1重组蛋白
  • 货号：
    CSB-EP761687HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人RAPH1重组蛋白
  • 货号：
    CSB-BP761687HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人RAPH1重组蛋白
  • 货号：
    CSB-MP761687HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    RAPH1
  • Uniprot No.：
    Q70E73
  • 别名：
    9430025M21Rik; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 18 protein; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 9 protein; C730009O10Rik; Lamellipodin; PREL-2; Proline-rich EVH1 ligand 2; Protein RMO1; RAPH1; RAPH1_HUMAN; Ras-associated and pleckstrin homology domains-containing protein 1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Mediator of localized membrane signals. Implicated in the regulation of lamellipodial dynamics. Negatively regulates cell adhesion.
  • 基因功能参考文献：
    1. The human RAPH1 gene is the CHE2 locus. PMID: 27346732
    2. these studies demonstrate an insight into how the Lpd-PH domain regulates cellular signals in a PI(3,4)P2 dependent manner. PMID: 26726010
    3. The authors propose that Lpd delivers Ena/VASP proteins to growing barbed ends and increases their actin polymerase activity by tethering them to actin filaments. PMID: 26295568
    4. These data indicate a possible role for Lpd in the actin-based movement and the cell-to-cell spread of L. monocytogenes. PMID: 26169271
    5. The results suggest that although the RAPH1 gene was deleted or amplified in all samples, the Lpd does not seem to play a major role in tumorigenesis of mammary carcinomas. PMID: 24057252
    6. The crystal structure of Lpd cc-RA-PH presents a molecular model of Lpd enhances actin polymerization by oligomerization via two intermolecular contact sites. PMID: 23483482
    7. results of this study suggested that that PI(3,4)P2, Lpd, and Ena/VASP are involved in the process movement of multipolar migrating cells. PMID: 22915108
    8. Nephrin ligation resulted in abnormal morphology of actin tails in human podocytes when Ship2, Filamin or Lamellipodin were individually knocked down. PMID: 22194892
    9. Data show that profilin1 negatively regulates lamellipodin targeting to the leading edge; profilin1 depletion increases lamellipodin concentration at the lamellipodial tip (where it binds Ena/VASP), and this mediates the hypermotility. PMID: 21115820
    10. RMO1 is expressed and deleted in osteosarcoma PMID: 15609301
    11. proline-rich peptides organize the 4 subunits of BChE into a 340 kDa tetramer, by interacting with the C-terminal BChE tetramerization domain PMID: 18076380
    12. Consistently, in HeLa cells, lamellipodin was required for EGF-induced proliferation. PMID: 19000833

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  • 亚细胞定位：
    Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, lamellipodium. Cell projection, filopodium. Cytoplasm, cytoskeleton. Note=Recruited to the membrane, via the PH domain, by the phosphoinositide, PI(3,4)P2. Colocalizes with ENAH/VASP at the tips of lamellipodia and filopodia. Also colocalizes with the pathogens, Vaccinia and Enteropathogenic E.coli (EPEC) at the interface between the pathogen and their actin.
  • 蛋白家族：
    MRL family
  • 组织特异性：
    Isoform RMO1-RAPH1 is ubiquitously expressed with highest levels in brain, heart, ovary and developing embryo. Isoform RMO1 is widely expressed with highest levels in liver. Low expression in B-cells.
  • 数据库链接：

    HGNC: 14436

    OMIM: 609035

    KEGG: hsa:65059

    STRING: 9606.ENSP00000316543

    UniGene: Hs.471162

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