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  4. Recombinant Human Protein CASC5 (CASC5), partial

Recombinant Human Protein CASC5 (CASC5), partial

  • 中文名称：
    人KNL1重组蛋白
  • 货号：
    CSB-YP854122HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人KNL1重组蛋白
  • 货号：
    CSB-EP854122HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人KNL1重组蛋白
  • 货号：
    CSB-EP854122HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人KNL1重组蛋白
  • 货号：
    CSB-BP854122HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人KNL1重组蛋白
  • 货号：
    CSB-MP854122HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    KNL1
  • Uniprot No.：
    Q8NG31
  • 别名：
    KNL1; CASC5; KIAA1570; Kinetochore scaffold 1; ALL1-fused gene from chromosome 15q14 protein; AF15q14; Bub-linking kinetochore protein; Blinkin; Cancer susceptibility candidate gene 5 protein; Cancer/testis antigen 29; CT29; Kinetochore-null protein 1; Protein CASC5; Protein D40/AF15q14
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Performs two crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Required for attachment of the kinetochores to the spindle microtubules. Directly links BUB1 and BUB1B to kinetochores. Part of the MIS12 complex, which may be fundamental for kinetochore formation and proper chromosome segregation during mitosis. Acts in coordination with CENPK to recruit the NDC80 complex to the outer kinetochore.
  • 基因功能参考文献：
    1. Mutations in the CASC5 gene were found to encode a kinetochore protein essential for mitotic cell division and to cause autosomal recessive primary microcephaly 4. (Review) PMID: 28901661
    2. Therefore, Mps1 promotes checkpoint activation through sequentially phosphorylating Knl1, Bub1, and Mad1. This sequential multi-target phosphorylation cascade makes the checkpoint highly responsive to Mps1 and to kinetochore-microtubule attachment. PMID: 28072388
    3. CASC5 may contribute to the morphological and structural changes of the human brain during recent evolution. The observed between-population divergence of CASC5 variants was driven by natural selection that tends to favor a larger gray matter volume in East Asians. PMID: 27878434
    4. Involvement of CASC5 in autosomal recessive microcephaly and a founder effect of the c.6125G>A mutation. PMID: 26626498
    5. Targeted Knockdown of the Kinetochore Protein D40/Knl-1 Inhibits Human Cancer in a p53 Status-Independent Manner PMID: 26348410
    6. We propose that CASC5 has a key role for the correct functioning of DNA damage response proteins, and a defect in this connection might affect upstream and downstream DNA damage response events as response to increased genotoxic stress. PMID: 26621532
    7. Data show sequential multisite regulation of the microtubule-associated protein KNL1-kinase-adaptor complex BUB1/BUB3 interaction. PMID: 25661489
    8. Mechanisms of mitosis-specific assembly of the checkpoint platform Knl1/MIS12/NDC80 at human kinetochores. PMID: 25601404
    9. PP2A-B56 is a key phosphatase for the removal of the Mps1-mediated Knl1 phosphorylations necessary for Bub1/BubR1 recruitment in mammalian cells. PMID: 25246613
    10. Protein phosphatase 1 (PP1) binding to KNL1 during prometaphase reduces the levels of Bub proteins at kinetochores to approximately the level recruited by four active MELT repeats. PMID: 24363448
    11. In cells depleted of endogenous Knl1, kinetochore-targeted Knl1(1-250) suffices to restore spindle assembly checkpoint and chromosome alignment PMID: 24361068
    12. KNL1 contains an extensive array of short linear sequence modules that encompass TxxOmega and MELT motifs and that can independently localize BUB1. PMID: 24344183
    13. KNL1 is a requirement for Aurora B activity at kinetochores and for wild-type kinetochore-MT attachment dynamics. PMID: 24344188
    14. Data provide strong evidence for CASC5 as a novel MCPH gene, and underscore the role of kinetochore integrity in proper volumetric development of the human brain. PMID: 22983954
    15. we identify the Blinkin motif critical for interaction with BUBR1, define the stoichiometry and affinity of the interaction, and present a 2.2 Angstrom resolution crystal structure of the complex PMID: 22000412
    16. Expression levels of D40 mRNA and proteins decrease according to the degree of spermatogenesis impairment in male infertile patients. PMID: 21272090
    17. h-KNL1 functions to connect Bub1 and BubR1 with the hMis12, Ndc80, and Zwint-1 complexes. PMID: 21199919
    18. phosphorylation of KNL1 by Aurora B disrupts the KNL1-PP1 interaction PMID: 20231380
    19. a new cancer/testis gene frequently expressed in lung cancer of smokers PMID: 12087463
    20. AF15q14 seems identical to an mRNA previously found to be expressed in melanoma rendered nontumorigenic by microcell-mediated introduction of normal chromosome 6, suggesting the gene may function normally to suppress cell growth and/or enhance maturation. PMID: 12618768
    21. Blinkin may be the center of the network for generating kinetochore-based checkpoint signaling PMID: 17981135
    22. the vertebrate KNL1 counterpart is essential for chromosome segregation and is required to localize a subset of outer kinetochore proteins. PMID: 18045986
    23. c-Abl and D40 interact with the tumor suppressor pRb protein and subsequently can lead to regulation of the cell proliferation. PMID: 18771174

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  • 相关疾病：
    Microcephaly 4, primary, autosomal recessive (MCPH4)
  • 亚细胞定位：
    Nucleus. Chromosome, centromere, kinetochore. Note=Weakly expressed in interphase nuclei. Expression increases from prophase to late anaphase, but greatly diminishes from the telophase and cytokinesis to early G1 phase of cell cycle.
  • 组织特异性：
    Highly expressed in testis, where it is localized in germ cells, in particular in spermatocytes and in the pre-acrosome of round spermatids. Detected in the acrosome of ejaculated spermatozoa. Detected in adult thymus, bone marrow, colon, small intestine,
  • 数据库链接：

    HGNC: 24054

    OMIM: 604321

    KEGG: hsa:57082

    STRING: 9606.ENSP00000335463

    UniGene: Hs.181855

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