Recombinant Human Keratin, type II cuticular Hb5 (KRT85)

中文名称：

人KRT85重组蛋白
货号：

CSB-YP012580HU
规格：

￥2616
图片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

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纯度：

Greater than 85% as determined by SDS-PAGE.
基因名：

KRT85
Uniprot No.：

P78386
种属：

Homo sapiens (Human)
蛋白长度：

Full Length
来源：

Yeast
分子量：

57.1 kDa
表达区域：

1-507aa
氨基酸序列

MSCRSYRISSGCGVTRNFSSCSAVAPKTGNRCCISAAPYRGVSCYRGLTGFGSRSLCNLGSCGPRIAVGGFRAGSCGRSFGYRSGGVCGPSPPCITTVSVNESLLTPLNLEIDPNAQCVKQEEKEQIKSLNSRFAAFIDKVRFLEQQNKLLETKWQFYQNQRCCESNLEPLFSGYIETLRREAECVEADSGRLASELNHVQEVLEGYKKKYEEEVALRATAENEFVVLKKDVDCAYLRKSDLEANVEALVEESSFLRRLYEEEIRVLQAHISDTSVIVKMDNSRDLNMDCIIAEIKAQYDDVASRSRAEAESWYRSKCEEMKATVIRHGETLRRTKEEINELNRMIQRLTAEIENAKCQRAKLEAAVAEAEQQGEAALSDARCKLAELEGALQKAKQDMACLLKEYQEVMNSKLGLDIEIATYRRLLEGEEHRLCEGVGSVNVCVSSSRGGVSCGGLSYSTTPGRQITSGPSAIGGSITVVAPDSCAPCQPRSSSFSCGSSRSVRFA
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
蛋白标签：

N-terminal 6xHis-tagged
产品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
缓冲液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
复溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
储存条件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保质期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
货期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
注意事项：

Repeated freezing and thawing is not recommended. Store working aliquots at 5°C for up to one week.
Datasheet & COA：

Please contact us to get it.

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■ 常见问题解答

蛋白纯化方法是什么，用什么缓冲

我们蛋白分离纯化用的是Ni柱亲和层析方法，大致步骤是首先用缓冲液平衡柱子，上样，然后用不同梯度洗脱液进行洗脱，收集相应洗脱峰并检测目的条带。洗脱液用的是NTA-0缓冲：20 mM Tris-HCl, 0.5 M NaCl，含有对应浓度的咪唑。

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基因功能参考文献：
1. Our study confirms linkage of a variant clinical form of hair-nail ectodermal dysplasia to chromosome 12 without any mutation in the coding sequences of the KRTHB5 gene. PMID: 20409997
2. crucial role of K85 in keratinization of hair and nails as shown by homozygous mutations in families PMID: 19865094
3. Direct evidence relating to the molecular pathogenesis of pure hair-nail ectodermal dysplasias with KRTHB5 gene mutation. PMID: 16525032
4. Transfected p65 induces transcriptional activation of Hb5. PMID: 18021261
相关疾病：

Ectodermal dysplasia 4, hair/nail type (ECTD4)
蛋白家族：

Intermediate filament family
组织特异性：

Synthesis occurs immediately above a small population of matrix cells at the base of the hair bulb and the trichocytes lining the dermal papilla and extends upward through the matrix and ends in the lower part of the cortex of the hair shaft.
数据库链接：

HGNC: 6462

OMIM: 602032

KEGG: hsa:3891

STRING: 9606.ENSP00000257901

UniGene: Hs.182507

Recombinant Human Keratin, type II cuticular Hb5 (KRT85)

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