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  4. Recombinant Human Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 (GBF1), partial

Recombinant Human Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 (GBF1), partial

  • 中文名称：
    人GBF1重组蛋白
  • 货号：
    CSB-YP846077HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人GBF1重组蛋白
  • 货号：
    CSB-EP846077HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人GBF1重组蛋白
  • 货号：
    CSB-EP846077HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人GBF1重组蛋白
  • 货号：
    CSB-BP846077HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人GBF1重组蛋白
  • 货号：
    CSB-MP846077HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    GBF1
  • Uniprot No.：
    Q92538
  • 别名：
    ARF1GEF; BFA resistant GEF 1; BFA-resistant GEF 1; FLJ21263; FLJ21500; GBF1; GBF1_HUMAN; golgi brefeldin A resistant guanine nucleotide exchange factor 1; Golgi specific brefeldin A resistance factor 1; Golgi specific Brefeldin A-resistance factor; Golgi-specific brefeldin A-resistance guanine nucleotide exchange; Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1; KIAA0248; MGC134877; MGC134878
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

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GBF1 antibodies

靶点详情

  • 功能：
    Guanine-nucleotide exchange factor (GEF) for members of the Arf family of small GTPases involved in trafficking in the early secretory pathway; its GEF activity initiates the coating of nascent vesicles via the localized generation of activated ARFs through replacement of GDP with GTP. Recruitment to cis-Golgi membranes requires membrane association of Arf-GDP and can be regulated by ARF1, ARF3, ARF4 and ARF5. Involved in the recruitment of the COPI coat complex to the endoplasmic reticulum exit sites (ERES), and the endoplasmic reticulum-Golgi intermediate (ERGIC) and cis-Golgi compartments which implicates ARF1 activation. Involved in COPI vesicle-dependent retrograde transport from the ERGIC and cis-Golgi compartments to the endoplasmic reticulum (ER). Involved in the trans-Golgi network recruitment of GGA1, GGA2, GGA3, BIG1, BIG2, and the AP-1 adapter protein complex related to chlathrin-dependent transport; the function requires its GEF activity (probably at least in part on ARF4 and ARF5). Has GEF activity towards ARF1. Has in vitro GEF activity towards ARF5. Involved in the processing of PSAP. Required for the assembly of the Golgi apparatus. The AMPK-phosphorylated form is involved in Golgi disassembly during mitotis and under stress conditions. May be involved in the COPI vesicle-dependent recruitment of PNPLA2 to lipid droplets; however, this function is under debate. In neutrophils, involved in G protein-coupled receptor (GPCR)-mediated chemotaxis und superoxide production. Proposed to be recruited by phosphatidylinositol-phosphates generated upon GPCR stimulation to the leading edge where it recruits and activates ARF1, and is involved in recruitment of GIT2 and the NADPH oxidase complex. Plays a role in maintaining mitochondrial morphology.
  • 基因功能参考文献：
    1. Experiments confirmed that GBF1 is required for hepatitis E virus replication. PMID: 29112323
    2. Study provides evidence that GBF-1 is required in endoplasmic reticulum structure and endosomal traffic. PMID: 23840591
    3. GBF1 could regulate different metabolic pathways through the activation of different pairs of Arf proteins. PMID: 26814617
    4. Oligomerization appears to stabilize GBF1 in cells. PMID: 26718629
    5. GBF1 plays a critical role early in dengue infection that is independent of its role in the maintenance of Golgi structure. PMID: 24855065
    6. The role of GBF1 in poliovirus replication is independent of its Arf activating function. PMID: 25653442
    7. Although human rhinovirus 3A protein was previously shown to interact with ACBD3, these data suggest that PI4KIIIbeta recruitment occurred independently of both GBF1 and ACBD3. PMID: 25410869
    8. GBF1 causes increased Arf-GTP production at the Golgi, consistent with a feed-forward self-limiting mechanism of Arf activation. PMID: 24213530
    9. These data suggest that AMPK-GBF1-Arf1 signaling is involved in the regulation of Golgi fragmentation during mitosis. PMID: 23418352
    10. an early acting GEF (GBF1) activates ARFs that mediate recruitment of late acting GEFs (BIG1/2) to coordinate coating events within the pre-Golgi/Golgi/TGN continuum. PMID: 23386609
    11. GBF1-mediated Arf1 activation is necessary to unify cell polarity during chemotaxis PMID: 22573891
    12. the vesicular transport proteins ARF1 and GBF1 colocalized with PI4KIIIbeta and were both required for HCV replication PMID: 22359663
    13. The GBF1 participated in delivery of adipose triglyceride lipase (ATGL) onto the lipid droplets(LD) surface PMID: 22185782
    14. We hypothesize that sphingomyelin acquired by CERT-dependent transport of ceramide and subsequent conversion to SM is necessary for C. trachomatis replication whereas SM acquired by GBF1-dependent pathway is essential for inclusion growth and stability. PMID: 21909260
    15. The COG (conserved oligomeric Golgi) complex plays a role in the localization, but not membrane association of GBF1. PMID: 21722633
    16. GBF1 and ATGL interact directly and in cells, through multiple contact sites on each protein. PMID: 21789191
    17. The brefeldin A block of poliovirus replication is rescued by expression of only the N-terminal region of GBF1 lacking the Sec7 domain. PMID: 20497182
    18. COPII is the only coat required for sorting and export from the endoplasmic reticulum exit sites, whereas GBF1, but not BIGs, is required for COPI recruitment, Golgi subcompartmentalization, and cargo progression to the cell surface. PMID: 18003980
    19. Both human and canine GBF1 can be inhibited by compound LG186, a small molecule derived from Exo2, a small molecule inhibitor of secretion and Shiga toxin cytotoxicity. PMID: 20854417
    20. Data suggest that Rab1 contributes to the specificity and timing of GBF1 recruitment by activating PI4KIIIalpha, and that the PtdIns(4)P produced then allows GBF1 to bind to Golgi membranes and activate Arf1. PMID: 20530568
    21. The authors demonstrate that Rab1b-activated GBF1 and ARF1 are involved in Ebolavirus virion formation, suggesting that both the COPII and COPI transport systems play a role in Ebolavirus VP40-mediated particle formation. PMID: 20164217
    22. Data propose that the phosphorylation and membrane dissociation of GBF1 and the consequent reduction in ARF-GTP levels in mitosis are important for changes in Golgi dynamics PMID: 20175751
    23. GBF1 is a cellular factor required for HCV infection. PMID: 19906930
    24. detailed subcellular localization to the cis-Golgi PMID: 12047556
    25. novel interaction between p115 and Golgi-specific brefeldin-A-resistant factor 1 (GBF1) PMID: 12634853
    26. characterization of alternatively spliced and truncated forms of GBF1 defines regions important for activity PMID: 12646181
    27. an Arf1-GBF1-Brefeldin A complex is formed and has a longer residence time on Golgi membranes than GBF1 or Arf1 alone PMID: 15616190
    28. A continuous cycle of recruitment and dissociation of GBF1 to membranes is required for sustained ARF activation and COP I recruitment that underlies ER-Golgi traffic. PMID: 15813748
    29. GBF1 is recruited to the endogenous IRF-9 promoter, and interacts with C/EBP-beta, IL-1, and IL-6 PMID: 16318580
    30. These observations strongly suggest that GBF1 regulates COPI membrane recruitment in the early secretory pathway. PMID: 16926190
    31. Two different poliovirus proteins independently recruit different Arf GEFs (GBF1 and BIG1/2) to membranes as part of cellular pathways utilized by the virus to form its membranous replication complex. PMID: 17079330
    32. analysis of the binding domain in GBF1 showed that the extreme N terminus, the dimerization/cyclophilin binding domain, and the homology upstream of Sec7 domain are required for the interaction with coxsackievirus protein 3A PMID: 17329336
    33. These data support a model where Rab1b-GTP induces GBF1 recruitment at the ERES interface and at the Golgi complex where it is required for COPII/COPI exchange or COPI vesicle formation, respectively. PMID: 17429068
    34. Required for GGA adaptor protein recruitment to Golgi membranes; plays a role in the proper processing and sorting of lysosomal cargo. PMID: 17666033
    35. These findings suggest that a secretory pathway capable of trafficking soluble proteins can be maintained in cells in which COPI recruitment is compromised by GBF1 depletion. PMID: 17956946
    36. GBF1 is a AMPK substrate and AMPK-mediated phosphorylation of GBF1 at Thr(1337) has a critical role, presumably by attenuating function of GBF1, in disassembly of the Golgi apparatus induced under stress conditions that lower the intracellular ATP PMID: 18063581
    37. Unfolded protein response and cell death after depletion of brefeldin A-inhibited guanine nucleotide-exchange protein GBF1. PMID: 18287014
    38. GDP-bound class II Arfs associate with the ER-Golgi intermediate compartment independently of GBF1 PMID: 18524849
    39. siRNAs demonstrate that GBF1-mediated ARF1 activation is required for efficient MHV RNA replication PMID: 18551169
    40. AG1478 inhibits GBF1, a large nucleotide exchange factor for the ADP-ribosylation factor, in a Sec7 domain-dependent manner and mimics the phenotype of a GBF1 mutant that has an inactive mutation PMID: 18799457
    41. GBF1 is responsible for the sensitivity of poliovirus infection to BFA, and is required for virus replication PMID: 19023417
    42. crucial for coxsackievirus B3 RNA replication PMID: 19740986

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  • 亚细胞定位：
    Golgi apparatus, cis-Golgi network. Endoplasmic reticulum-Golgi intermediate compartment. Golgi apparatus, trans-Golgi network. Golgi apparatus. Cytoplasm. Lipid droplet. Membrane; Peripheral membrane protein.
  • 组织特异性：
    Ubiquitous.
  • 数据库链接：

    HGNC: 4181

    OMIM: 603698

    KEGG: hsa:8729

    STRING: 9606.ENSP00000359000

    UniGene: Hs.290243

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