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  4. Recombinant Human Exonuclease 1 (EXO1), partial

Recombinant Human Exonuclease 1 (EXO1), partial

  • 中文名称：
    人EXO1重组蛋白
  • 货号：
    CSB-YP007876HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人EXO1重组蛋白
  • 货号：
    CSB-EP007876HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人EXO1重组蛋白
  • 货号：
    CSB-EP007876HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人EXO1重组蛋白
  • 货号：
    CSB-BP007876HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人EXO1重组蛋白
  • 货号：
    CSB-MP007876HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    EXO1
  • Uniprot No.：
    Q9UQ84
  • 别名：
    exo1; EXO1_HUMAN; ExoI; Exonuclease 1; Exonuclease I; Exonuclease1; HEX1; hExo I; hExo1; hExoI; Rad2 nuclease family member homolog of S. cerevisiae exonuclease 1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

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EXO1 antibodies

靶点详情

  • 功能：
    5'->3' double-stranded DNA exonuclease which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. Functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes. Essential for male and female meiosis.
  • 基因功能参考文献：
    1. These findings provide the evidence that the rs1047840, rs9350, rs10802996, rs1635498, rs1776148, rs1776177, rs3754093 and rs851797 polymorphisms may act as risk factors for cancer. PMID: 27387683
    2. The results from gene-environment interaction assay indicated all hEXO1 SNPs interacted with smoking, alcohol consumption, HBV infection in pathogenesis of hepatocellular carcinoma. PMID: 27894089
    3. The finding that a threading mechanism like that used by hFEN1 is also used by hEXO1 unifies the mode of operation for members of the 5'-nuclease superfamily that act on discontinuous substrates. As with hFEN1, intrinsic disorder of the arch region of the protein may explain how flaps can be threaded without a need for a coupled energy source. PMID: 28682061
    4. These findings indicate that the coupling of EXO1 activation with its eventual degradation is a timing mechanism that limits the extent of DNA end resection for accurate DNA repair. PMID: 28515316
    5. Mutations in the human EXO1 gene correlate with increased susceptibility to some cancers. This review summarizes recent studies on the enzymatic functions and biological roles of EXO1, its possible protective role against cancer and aging, and regulation of EXO1 by posttranslational modification. [review] PMID: 27494243
    6. These results suggest that Metnase enhances Exo1-mediated exonuclease activity on the lagging strand DNA by facilitating Exo1 loading onto a single strand gap at the stalled replication fork. PMID: 27974460
    7. we present evidence that the inherited EXO1 polymorphism rs9350 may have a substantial influence on prostate cancer risk in Chinese people. We believe that the rs9350 could be a useful biomarker for assessing predisposition for and early diagnosis of prostate cancer. PMID: 26646562
    8. study concludes that Exo1 gene polymorphism Lys589Glu may be associated with an increased risk of colorectal cancer in the Polish population PMID: 25294706
    9. Mutations within the EXO1 gene are not associated with premature ovarian failure in Han Chinese women. PMID: 26774993
    10. EXO1 is more active on DNA at temperatures below 37 degrees C and this manifests as an increase in nuclease activity.EXO1 preferentially cleaves one nucleotide inwards in a double stranded region of forked and nicked DNA flap substrates. PMID: 26182368
    11. MSH2, MSH6, and EXO1 genes were overexpressed in gastroesophageal cancers. PMID: 26215063
    12. EXO1 and FEN1 cleaved the substrate at the boundary between the single-stranded 5' flap and the duplex, whereas FAN1 incised it three to four nucleotides in the double-stranded region. PMID: 26221031
    13. In summary, phosphorylation of EXO1 by CDKs is a novel mechanism regulating repair pathway choice. PMID: 24705021
    14. Our data present, for the first time, evidence that inherited MLH1 c.-93G>A, MSH2 c.211 + 9C>G, MSH3 c.3133G>A, and EXO1 c.1765G>A abnormalities of DNA MMR pathway are important determinants of head and neck squamous cell carcinoma PMID: 25598504
    15. 14-3-3 proteins restrain the Exo1 nuclease to prevent over-resection in end joining PMID: 25833945
    16. These studies establish that the Mlh1-Pms1 endonuclease is required for MMR in a previously uncharacterized Exo1-independent MMR pathway. PMID: 24204293
    17. EXO1 Glu589Lys polymorphism is not associated with overall cancer susceptibility. PMID: 24761866
    18. FOXM1 is upregulated in chemoresistant ovarian cancer samples, and defends ovarian cancer cells against cytotoxicity of cisplatin. FOXM1 facilitates DNA repair through regulating EXO1 to protect ovarian cancer cells from cisplatin-mediated apoptosis. PMID: 24824601
    19. This study reports that, contrary to earlier reports, and unlike the catalytic site mutant D173A, the EXO1 E109K variant resembled the wild-type enzyme on all tested substrates. PMID: 24829445
    20. In G1 arrested cells that histone H2A phosphorylation, in response to ionizing radiation, is independent of Sgs1 and Exo1. PMID: 23835406
    21. This mouse transgene line carries an Exo1-null knockout mutation leading to the complete loss of EXO1 protein expression (termed Exo1(null); Exo1-mutant lines show defects in DNA damage response. PMID: 23754438
    22. EXO1 deficiency leads to ICL sensitivity but does not increase ICL sensitivity in the absence of FANCD2. PMID: 22987153
    23. No statistically significant differences were found in the allele or genotype distributions of the Exo 1 T439M polymorphism among HCC and cancer-free control subjects (P>0.05). PMID: 22296401
    24. direct and robust interaction between hEXO1 and six of the seven 14-3-3 isoforms, is demonstrated. PMID: 22222486
    25. A promoter region SNP in the editing and processing nucleases gene EXO1 was associated with decreased expression of EXO1 and decreased melanoma risk. PMID: 22230721
    26. Exo1 as a key mediator of DNA end resection and DSB repair and damage signaling decisions in human cells PMID: 22326273
    27. In this study, we have chosen 2 common SNPs of EXO1 and investigated the association between these 2 SNPs and the risk for cervical cancer in Chinese population by PCR-RFLP. PMID: 22146767
    28. The Lys/Lys homozygote genotype of Exo 1 K589E polymorphism may be a genetic susceptibility factor for hepatocellular carcinoma in the Turkish population. PMID: 22205538
    29. Gene expression intensity of exonuclease 1 as related to Aicardi- Goutie;res syndrome mutations. PMID: 21862834
    30. Human Ku70/80 protein blocks exonuclease 1-mediated DNA resection in the presence of human Mre11 or Mre11/Rad50 protein complex. PMID: 22179609
    31. a key role for hEXO1 in the UV-induced DNA damage response linking nucleotide excision repair to checkpoint activation in human cells. PMID: 21808022
    32. Exo1 induces a sharp bend in the DNA at nicks or gaps. Frayed 5' ends of nicked duplexes resemble flap junctions, unifying the mechanisms of endo- and exonucleolytic processing. PMID: 21496642
    33. BLM increases the affinity of EXO1 for ends, and MRN recruits and enhances the processivity of EXO1. PMID: 21325134
    34. Gene-environment interactions with smoking were significant for Exo1 K589E polymorphism (OR=2.509, 95% CI=1.914-3.287) and results provide evidence that the A allele of the Exo1 K589E may be associated with the development of oral cancer. PMID: 19515603
    35. This study identifies new functions of CtIP and EXO1 in DNA end resection and provides new information on the regulation of DNA double-strand breaks repair pathways, which is a key factor in the maintenance of genome integrity. PMID: 21052091
    36. The EXO1 polymorphism, P757L, was analyzed by PCR-RFLP. Patients with the Leu/Leu genotype have a reduced risk of colorectal cancer. PMID: 20854105
    37. study found in gastric and colorectal cancers that EXO1 gene harbored somatic frameshift mutations within the mononucleotide repeats in the coding sequences; these mutations were found in the cancers with microsatellite instability PMID: 20429728
    38. FEN1 and EXO1 can eliminate structures formed by trinucleotide repeats in the course of replication, relying on endonucleolytic and exonucleolytic activities, respectively. PMID: 20643645
    39. results suggest that EXO1 may act at transcription-induced telomeric structures to promote telomere recombination while FEN1 has a dominant role in lagging strand replication at telomeres PMID: 20126648
    40. Exo1 is phosphorylated after DNA damage and this event is required for the subsequent recruitment of other DNA repair proteins and homologous recombination PMID: 20019063
    41. The A allele of the Exo1 K589E may be associated with the development of gastric cancer and may be a novel and useful marker for primary prevention and anticancer intervention. PMID: 20337148
    42. requirement for in 5' and 3' mismatch repair PMID: 11809771
    43. structural analysis of interactions of Exo1 with DNA PMID: 11842105
    44. Functional alterations of human exonuclease 1 mutants identified in atypical hereditary nonpolyposis colorectal cancer syndrome. PMID: 12414623
    45. Several EXO1 variants observed in patients were also observed in controls with similar frequencies, including the truncating variant proposed previously to be a disease-causing mutation PMID: 12517792
    46. hPMS2 accounts for a small proportion of Hereditary non-polyposis colorectal cancer families, and none were deemed to be associated with hEXO1 PMID: 14756672
    47. REVIEW: role in cancer? PMID: 15328369
    48. there is an association of EXO1 gene polymorphisms with colorectal cancer risk. PMID: 15550454
    49. hEXO1, hMLH1 and hMSH2 form complexes and are imported to the nucleus together, and that redundant nuclear localization signals in the proteins may safeguard nuclear import and thereby mismatch repair activity. PMID: 17426132
    50. MLH3 and EXO1 alterations in familial colorectal cancer patients not fulfilling Amsterdam criteria. PMID: 17656264

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  • 亚细胞定位：
    Nucleus. Note=Colocalizes with PCNA to discrete nuclear foci in S-phase.
  • 蛋白家族：
    XPG/RAD2 endonuclease family, EXO1 subfamily
  • 组织特异性：
    Highly expressed in bone marrow, testis and thymus. Expressed at lower levels in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen and stomach.
  • 数据库链接：

    HGNC: 3511

    OMIM: 606063

    KEGG: hsa:9156

    STRING: 9606.ENSP00000311873

    UniGene: Hs.498248

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